In Vivo Analyses of Osteogenic Activity and Bone Regeneration Capacity of Demineralized Freeze-Dried Bovine Bone Xenograft: A Potential Candidate for Alveolar Bone Fillers

Abstract

Background. Deproteinized bovine bone mineral (DBBM) particle is the commonly used bone graft substitute in implant surgery which is mainly osteoconductive and has very slow degradation. Demineralized freeze-dried bovine bone xenograft (DFDBBX) particle is being developed as a novel xenogeneic bone filler. Objectives. The study aimed to analyze osteogenic activity and bone-forming capacity of DFDBBX particles compared to DBBM particles in alveolar bone defects in rabbit mandibles models. Material and Methods. This study investigated bone defects whether filled with DBBM particles or DFDBBX particles or left unfilled in 30 rabbit mandibles. Specimens were processed for histology, immunohistochemistry, and micro-CT scanning. Statistical difference was set at a p value < 0.05. Results. The quantitative assessment showed a significantly lower number of osteoclasts and a higher number of osteoblasts in the DFDBBX group compared to the DBBM group in 2 and 4 weeks ( p < 0.05 ). Immunostaining analyses showed significantly higher expression of RUNX2, collagen type I, alkaline phosphatase, and osteocalcin in the DFDBBX group compared to the DBBM group in 2 and 4 weeks. Bone healing score in the DFDBBX group was comparable to the DBBM group. Micro-CT presented no significant difference in the volume percentage of the mineralized tissue in the DBBM and DFDBBX groups in spite of the different healing patterns in both groups. Conclusion. DFDBBX particles induced higher osteoblastic activities than DBBM particles at the early stage of healing. Meanwhile, the capacity of bone formation in DFDBBX particles was comparable with DBBM particles at the later stage of healing. Considering the limitation of this study, the results presented DFDBBX particles as potential bone filler candidates.

Clinical application of concentrate growth factors combined with bone substitute in Alveolar ridge preservation of anterior teeth

“…The morphology of soft and hard tissue can be preserved to the greatest extent by reducing the bone loss of extraction socket and accelerating bone regeneration. There are many different ARP techniques and various types of materials, such as autogenous bone [ 5 ], allografts [ 6 ], xenografts and platelet concentrates [ 7 , 8 ]. The main goal of the bone graft material is to serve as a scaffold and maintain a space for bone ingrowth, blood vessels formation, to support soft tissues and to improve the quality and quantity of regenerated bone [ 9 ].…”

Evaluation of regeneration after the application of 2 types of deproteinized bovine bone mineral to alveolar bone defects in adult dogs

“…However, these non-mineralized bone materials have inferior mechanical properties. In contrast, DBBM, which is mineralized, has higher mechanical stability [ 27 ]. Bio-Oss, a bovine bone substitute, had significantly slower resorption than other bone graft materials [ 28 ].…”

The Osteogenesis Mechanisms of Dental Alveolar Bone Socket Post Induction with Hydroxyapatite Bovine Tooth Graft: An Animal Experimental in Rattus norvegicus Strain Wistar

“…HE staining was used to count the number of OBs and OCs. 16 IHC staining was used for examining BMP-2, OCN, OSX, and RUNX2 assay. 1,16,20,21 For the examination of BMP-2, OSX, OCN, and RUNX2 expressions, OB, and OC, we used a 1,000Â magnification light microscope in 20 microscopic fields.…”

“…16 IHC staining was used for examining BMP-2, OCN, OSX, and RUNX2 assay. 1,16,20,21 For the examination of BMP-2, OSX, OCN, and RUNX2 expressions, OB, and OC, we used a 1,000Â magnification light microscope in 20 microscopic fields. The mean results per microscopic field were tabulated and analyzed with SPSS version 25.…”