Spatial-Frequency Azimuthally Stable Cartography of Biological Polycrystalline Networks

Abstract

A new azimuthally stable polarimetric technique processing microscopic images of optically anisotropic structures of biological tissues histological sections is proposed. It has been used as a generalized model of phase anisotropy definition of biological tissues by using superposition of Mueller matrices of linear birefringence and optical activity. The matrix elementM44has been chosen as the main information parameter, whose value is independent of the rotation angle of both sample and probing beam polarization plane. For the first time, the technique of concerted spatial-frequency filtration has been used in order to separate the manifestation of linear birefringence and optical activity. Thereupon, the method of azimuthally stable spatial-frequency cartography of biological tissues histological sections has been elaborated. As the analyzing tool, complex statistic, correlation, and fractal analysis of coordinate distributions ofM44element has been performed. The possibility of using the biopsy of the uterine wall tissue in order to differentiate benign (fibromyoma) and malignant (adenocarcinoma) conditions has been estimated.

Biomedical applications of Jones-matrix tomography to polycrystalline films of biological fluids

“…Based on the synthesis of theoretical 30,31 and experimental [23][24][25][26][27][28][29]38,39 results, we obtained algorithms for complex Jones-matrix reconstruction of the distributions of both phase (LB 0;90 ; LB 45;135 ; CB ;È ) and amplitude (LD 0;90 ; LD 45;135 ; CD ;È ) anisotropy of polycrystalline¯lms of biological°u ids…”

“…Previously, we found that method of direct polarization mapping of microscopic images of biological°uid¯lms (blood plasma, synovial°uid, liquor) is sensitive to pathological changes in human organs. [23][24][25][26][27][28] Further development of these studies allowed the Jones-matrix reconstruction of the distributions of linear and circular birefringence of polycrystalline bile¯lms. 29 Thus, this method can be used for diagnostics of Type II diabetes with excellent accuracy.…”

Stokes-Correlometry Analysis of Biological Tissues With Polycrystalline Structure

“…HE optically-based polarimetry is popular experimental approach widely used for non-invasive diagnosis of various turbid tissue-like scattering media [1][2][3][4][5][6], including those containing optically anisotropic layers [7][8][9] and fluids [10][11][12]. With the recent instrumentation developments in the field, a high sensitivity of laser polarimetry in screening of turbid cancerous biological tissues has been achieved [13][14][15][16].…”

3D Mueller-Matrix Diffusive Tomography of Polycrystalline Blood Films for Cancer Diagnosis

“…In the frame of these studies, a development of algorithms for reconstructing the distributions of phase and amplitude anisotropy parameters of the protein polycrystalline networks of sampling layers have been performed. Later, the potential of this diagnostic approach has been demonstrated [24][25][26][27][28].…”