Biomedical applications of Jones-matrix tomography to polycrystalline films of biological fluids

Ушенко, В. А.; Sdobnov, Anton; Mishalov, W. D.; Dubolazov, A. V.; Олар, О. В.; Bachinskyi, V. T.; Ушенко, А. Г.; Wanchuliak, O. Y.; Meglinski, Igor

doi:10.1142/s1793545819500172

Cited by 35 publications

(32 citation statements)

References 40 publications

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“…An analogous finding has also been observed with reference to myocardium tissue with control and different pathological groups where the decrease of linear birefringence due to the randomized local orientation and shortening of the myosin fibrils has been reported . The polycrystalline films of biological fluids utilizing their Jones‐matrix tomograms has been reported where the reconstructed circular birefringence and dichroism prevails over the linear birefringence due to high concentration of optically active cholesterol monohydrate and calcium bilirubinate over the linearly birefringent needle crystal of fatty acids . In those reports, the various dependencies ( k a >, ~, < k b and n a >, ~, < n b where a and b are associated with linear, linear‐45 and circular polarization of states) of the real (phase anisotropy) and imaginary (amplitude anisotropy) parts of the refractive index have not been taken into account, but an overall average response of each type of the combination of the refractive index.…”

Section: Resultsmentioning

confidence: 98%

Mapping of retardance, diattenuation and polarizance vector on Poincare sphere for diagnosis and classification of cervical precancer

Zaffar

Pradhan

2020

Journal of Biophotonics

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The mapping of diattenuation, polarizance and retardance vector (normalized Stokes vector) on Poincare sphere, evaluated from Mueller matrix of optically anisotropic stromal region of cervical tissues, is presented for cervical precancer detection and its staging. This reveals that the changes in the polarization states shown by these normalized Stokes vectors corresponds to the degradation of linearly arranged collagen fibers, breakage of the collagen cross links in the stromal region and change in the density of scattering sites when cervical cancer evolves. The distinct nature of real and imaginary parts of the refractive index for linear, linear-45 and circular polarization from the optically anisotropic stromal region underscore the various polarization structures of the connective tissue region which get modified during the pathological changes. It has been found that versatility of these vectors for normal and precancerous cervical tissue of various grades may be utilized as a key distinction for qualitative staging of cervical precancer tissue. Quantitative classification of precancerous stages of cervical precancer has been determined with 95%-100% sensitivity and 93%-100% specificity through the evaluation of linear and circular diattenuation, linear polarizance and linear birefringence from the components of the respective vectors. K E Y W O R D S cervical cancer, diattenuation vector, Mueller matrix, Poincare sphere, polarizance vector, retardance vector, stromal region

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Section: Resultsmentioning

confidence: 98%

Mapping of retardance, diattenuation and polarizance vector on Poincare sphere for diagnosis and classification of cervical precancer

Zaffar

Pradhan

2020

Journal of Biophotonics

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“…This pretreatment increases the complexity of the operation and inevitably affects the physiological characteristics of biological cells. It is an urgent need for the research of life science to develop new imaging analysis technology to realize undisturbed, dynamic and quantitative analysis of living, cell and subcellular samples [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Application of Improved GS Algorithm In Cell Computing Holography

Wang

Guan

et al. 2021

Preprint

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Background: As an important research direction in cell image processing, Computer Hologram(CH) can quantitatively detect and analyze the amplitude and phase information of cells and holographic reconstruct the recorded images. Compared with the traditional optical microscope, CH reduces the complexity of operation, avoids the operation of cell staining and does not affect the physiological characteristics of cells in the recording process. It plays an important role in the field of cell morphology measurement and deformation analysis. Results: An improved Gerchberg-Saxton (GS) algorithm is proposed to reconstruct cell hologram based on phase information. The cell image reconstructed by GS Algorithm with phase restricted conditions are analyzed and compared. Through the comparative experiments of different models, it shows that the improved GS algorithm is better than the traditional GS algorithm in cell image reconstruction based on phase information. With the improved algorithm of phase mapping and normalization conditions, the reconstructed image can distinguish the cell edge information and high signal-to-noise ratio information. The urine sediment image is taken as the experimental object. After reconstruction by this algorithm, other impurity cells can be filtered out, and the aberrant red blood cell image with clear edge can be obtained. This improved algorithm provides a new application for cell detection in clinical diagnosis.Conclusions: The phase map reflects the temporal and spatial information of the image, determines the time or spatial position where different frequency signals appear, and describes the overall shape of the object. Due to biological cells generally for phase type or class phase objects, the improved GS algorithm in this paper can well describe the phase information of restored image, which is more suitable for the practical application of cell phase reconstruction.

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“…In the recently published studies, MM polarimetry has been applied to differentiating the polycrystalline structure of healthy and pathologically altered tissues of myocardium [13], characterization of rectum wall tissue, vaginal wall during genital prolapse, brain tissue [14], detection of healthy and pre-cancerous regions of uterine cervix tissue [15][16][17], blood films characterization for cancer diagnosis [18], detection of cancer of colorectal tissue [19,20] and characterization of other tissues [21,22]. Other polarization-based techniques, such as Stokes polarimetry based on circularly polarized illumination [23,24], Jones-matrix tomography [25,26], Stokes correlometry [27] were also widely used for the characterization of various tissues.…”

Section: Introductionmentioning

confidence: 99%

Evaluating β-amyloidosis progression in Alzheimer’s disease with Mueller polarimetry

Borovkova¹,

Bykov²,

Попов³

et al. 2020

Biomed. Opt. Express

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We applied the wide-field Mueller imaging polarimetry for the screening of formalinfixed paraffin-embedded samples of mouse brain tissue at different stages of brain β-amyloidosis in Alzheimer's disease (AD). The accumulation of amyloid-beta (Aβ) deposits throughout the brain tissue is one of the key pathological hallmarks observed with the AD progression. We demonstrate that the presence of Aβ plaques influences the properties of backscattered polarized light, in particular, its degree of depolarization. By means of statistical analysis, we demonstrate that the high-order statistical moments of depolarization distributions, acquired with the multi-spectral Mueller imaging polarimetry, can be used as sensitive markers of the growing presence of Aβ plaques. The introduced label-free polarimetric approach has a potential to facilitate the current practice of the histopathology screening in terms of diagnosis accuracy, time and cost efficiency.

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Biomedical applications of Jones-matrix tomography to polycrystalline films of biological fluids

Cited by 35 publications

References 40 publications

Mapping of retardance, diattenuation and polarizance vector on Poincare sphere for diagnosis and classification of cervical precancer

Mapping of retardance, diattenuation and polarizance vector on Poincare sphere for diagnosis and classification of cervical precancer

Application of Improved GS Algorithm In Cell Computing Holography

Evaluating β-amyloidosis progression in Alzheimer’s disease with Mueller polarimetry

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