Somatic embryogenesis in Rosa spp.

Somatic Embryogenesis and Plant Regeneration from in-vitro-grown Leaf Explants of Rose

“…Omission or lowering the concentration of 2,4-D in the culture medium encouraged embryo development and germination in many cultivars of Rosa species (Matthews et al, 1991;Roberts et al, 1990). Roberts et al (1995) also reported that chilling at 4 °C for 2 weeks improved the germination rates from 12% to 24 %. The combination of 6-benzyladenine (BA) with methyl laurate was observed to stimulate somatic embryo development in the cultivar Vague (Sarasan et al, 2001).…”

“…In roses, somatic embryogenesis has been obtained from a variety of explants such as calli derived from leaf tissue (De Wit et al, 1990), immature leaf and stem segments (Rout et al, 1991), immature seeds (Kunitake et al, 1993), petioles, roots (Marchant et al, 1996;Roberts et al, 1995), and anther fi laments (Noriega and Sondahl, 1991). According to published reports, callus initiation from rose tissues required the presence of an auxin (mainly 2,4-dichorophenoxy acetic acid (2,4-D) and α-naphthalene acetic acid (NAA)) in the culture…”

Plant regeneration through somatic embryogenesis and rapd analysis of regenerated plants in Tylophora indica (Burm. F. Merrill.)

“…Realizing this, Sharma and Chandel (1992) established a protocol for axillary bud induction and shoot multiplication from T. indica. However, plant regeneration via somatic embryogenesis is considered to be more efficient than shoot multiplication (Roberts et al, 1995) and opens up the prospects of applying recombinant DNA technology (Hassig et al, 1987). In addition, in medicinal plants, it has been reported that productivity in terms of quantity and quality of secondary metabolites was enhanced in plants derived from somatic embryogenesis (Gastaldo et al, 1994).…”

<i>In Vitro</i> Regeneration of <i>Curcuma caesia</i> Plantlets from Basal Part and via Somatic Embryogenesis

“…Of the two approaches, somatic embryogenesis is thought to be more efficient [4]. According to [5], some of the advantages somatic embryogenesis has over direct in vitro propagation systems include the former's high multiplication rates, possibility of cryopreservation of embryogenic callus, the potential for scale-up in liquid suspension cultures, amenability to the use of bioreactors and the application of somatic synthetic seed technologies.…”