Somatic Embryogenesis and Plant Regeneration from in-vitro-grown Leaf Explants of Rose

Kim, C.K.; Oh, Joo Yeon; Chung, Jen-Ping; Burrell, A. Millie; Byrne, David

doi:10.21273/hortsci.39.6.1378

Cited by 21 publications

(21 citation statements)

References 17 publications

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“…Despite the fact that somatic embryogenesis from explants grown and maintained in vitro was successfully reported in many plant species (e.g., rhizomatous Iris (Laublin et al 1991), Camellia sinensis O. Kunze (Kato 1996), Rosa hybrida and Rosa multiflora (Kim et al 2004), Pyrus communis L. (Javadi et al 2013) and Olea europaea L. ), this approach has been scarcely studied in date palm (Al-Mayahi 2015). Therefore, the aim of this investigation was to develop a reproducible somatic embryogenesis pathway from buds and shoots maintained in vitro of date palm cv.…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis from bud and leaf explants of date palm (Phoenix dactylifera L.) cv. Najda

et al. 2017

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An efficient regeneration system through somatic embryogenesis was developed for date palm cv. Najda. Adventitious bud and proximal leaf segments cultured on Murashige and Skoog (MS) medium supplemented with various combinations of auxins and cytokinins induced embryogenesis after at least 6 months of culture. Somatic embryogenesis induction seemed correlated with the type of the explant, the induction period and the auxin used. The highest rate of somatic embryogenesis (86.0%) was obtained on bud explants cultured on MS medium supplemented with 45.0 µM 2,4-dichlorophenoxyacetic acid (2,4-D), and 4.5 µM kinetin or 4.5 µM 6-(dimethylallylamino) purine (2iP). Whereas, low levels of embryogenesis were obtained on media supplemented with 1-naphthalene acetic acid (NAA) or 2-naphthoxyacetic acid (NOA). Proximal leaf segments showed somatic embryogenesis only when cultured on media supplemented with 2,4-D or picloram. Statistical analysis revealed significant effects of explant type and plant growth regulators (PGRs) combination on somatic embryogenesis. Somatic embryos were germinated successfully on PGR-free MS medium with or without activated charcoal (50.0-60.0 and 26.6-36.6%, respectively), and 80.0% of plantlets survived after transferring to a glasshouse for 6 months. Our results will be useful for large-scale propagation of date palm cv. Najda, characterized by high fruit quality and bayoud disease resistance.

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Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis from bud and leaf explants of date palm (Phoenix dactylifera L.) cv. Najda

et al. 2017

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“…There are several reports on adventitious shoot regeneration and somatic embryo formation from different explants of roses (Tweddle et al 1984;Lloyd et al 1988;Burger et al 1990;Noriega and Sondahl 1991;Kunitake et al 1993;Arene et al 1993;Firoozabady et al 1994;Rosu et al 1995;Hsia and Korban 1996;Kim et al 2003;Pati et al 2004), but most protocols are useful only for specific genotypes, or occur at such low frequencies thus limiting value of these protocols for most roses. Fortunately, researchers also succeeded in obtaining pure forms from chimeras by rapid proliferation in vitro (Hall et al 1986;McPheeters and Skirvin 1989;Rosu et al 1995).…”

Section: Introductionmentioning

confidence: 99%

In vitro separation of a rose chimera

Canli

Skirvin

2008

Plant Cell Tiss Organ Cult

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“…chinensis cv. Old Blush had previously been reported as producing non-embryogenic callus after induction of foliar explants with 4.5 lM 2,4-D hormone (Kim et al 2003). Here, the response of Old Blush callus to somatic embryogenic induction was triggered by replacing 2,4-D with 8.06 lM NAA.…”

Section: Genetic Transformation Assays Of Rcobtype2 Embryosmentioning

confidence: 93%

Somatic embryogenesis and transformation of the diploid Rosa chinensis cv Old Blush

Vergne

Maene

Gabant

et al. 2009

Plant Cell Tiss Organ Cult

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International audienceSomatic embryogenesis was induced from in vitro-derived leaf explants of Rosa chinensis cultivar (cv) Old Blush. Calli producing embryos with expanded cotyledons (RcOBType1 embryos) were obtained. Further refinements of the callus maintenance medium generated a more typical rose embryogenic callus (RcOBType2) displaying high levels of secondary embryogenesis and embryos with limited cotyledon expansion Agrobacterium tumefaciens-mediated transformation assays using β-glucuronidase (GUS) reporter gene showed that both types of embryos were competent for transformation. Under selection conditions, transformed RcOBType1 explants produced non chimaeric transformed embryos, from which shoots could be adventitiously regenerated. In contrast to RcOBType1, transformed RcOBType2 embryos directly yielded transformed shoots when repeatedly cultured in selective regeneration conditions. Transformation efficiency ranged between three to nine percent and shoots suitable for rooting were obtained within 6-8 months. Transgenic plants were transferred into the greenhouse and molecularly confirmed. The availability of transformation methods in a diploid rose, R. chinensis cv. Old Blush, will be useful for gene functional studies

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Somatic Embryogenesis and Plant Regeneration from in-vitro-grown Leaf Explants of Rose

Cited by 21 publications

References 17 publications

Somatic embryogenesis from bud and leaf explants of date palm (Phoenix dactylifera L.) cv. Najda

Somatic embryogenesis from bud and leaf explants of date palm (Phoenix dactylifera L.) cv. Najda

In vitro separation of a rose chimera

Somatic embryogenesis and transformation of the diploid Rosa chinensis cv Old Blush

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