In vitro separation of a rose chimera

Canli, Fatih Ali; Skirvin, R. M.

doi:10.1007/s11240-008-9449-y

Cited by 7 publications

(4 citation statements)

References 22 publications

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“…of plantlets examined in Tables 1-4) differences. This was different from previous studies on other chimeras that BA concentration was the main factor influencing the separation of a rose chimera (Canli and Skirvin, 2008). It has been reported that higher ploidy level plants need higher BA concentrations than diploid plants in in vitro proliferation (Chen et al, 2012;Nilanthi et al, 2009b).…”

Section: Discussioncontrasting

confidence: 88%

“…Segregation of chimeras into pure plants containing only one type of component cells is a long-established topic (Canli, 2003;Canli and Skirvin, 2008;Chua et al, 1981;Hall et al, 1986;Marcotrigiano et al, 1987;Skirvin, 1983, 1989;Ning et al, 2009;Peary et al, 1988;Rosu et al, 1995;Roy et al, 2001). The mechanism was primarily explained using the theory of the tunica-corpus pattern of the meristematic region described by Schmidt (1924) and later refined by Tilney-Bassett (1986, 1991.…”

Section: Discussionmentioning

confidence: 99%

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In Vitro Segregation of Tetraploid and Octoploid Plantlets from Colchicine-induced Ploidy Chimeras in Echinacea purpurea L.

Li¹,

Yang²,

Wu³

2016

horts

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Echinacea purpurea L. is one of the important ornamental and medicinal plant species. Ploidy manipulation is a valuable tool for improving plant quality or production in E. purpurea as well as in many other plants. To study the segregation of pure ploidy plantlets from colchicine-induced ploidy chimeras in E. purpurea, we used a chimera plantlet that consisted of 1.93% diploid, 35.04% tetraploid, and 63.03% octoploid cells as the source material for experiments. The results showed that three factors significantly influenced the segregation, i.e., the component ratios of different ploidy cells in the chimera, the number of sequential passages, and the methods of segregation culture of the chimera plantlets. Other factors, such as explant types (i.e., leaf, petiole, or root) and 6-benzyladenine (BA) concentrations (i.e., 0.2, 0.4, 0.8, and 1.2 mg·L−1) occasionally influenced the segregation. Pure chromosome-doubled polyploids are not easily obtained in various plant species, so segregation culture of ploidy chimeras may potentially be more effective. The morphological characteristic and content of cichoric acid were compared among diploid, tetraploid, and octoploid plants. Results indicated that tetraploid and octoploid plants had more stunted growth, larger stomata, lower stomata frequency, more chloroplast number in guard cells, and higher cichoric acid content than original diploid lines.

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Section: Discussioncontrasting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

In Vitro Segregation of Tetraploid and Octoploid Plantlets from Colchicine-induced Ploidy Chimeras in Echinacea purpurea L.

Li¹,

Yang²,

Wu³

2016

horts

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“…The hypothesis of separation of chimera components is supported by the results of Zalewska et al (2007), who by using the same cultivars obtained a separation among plants produced from leaf explants; plants of 'Lady Apricot' produced purple and purple-gold inflorescences, and of 'Lady Salmon' pink and white. Chimera compounds separation was also reported by Canli and Skirvin (2008) for Rosa multiflora Thunb ex. J. Murr.…”

Section: Ex Vitro Growth and Inflorescence Colour Of Plantsmentioning

confidence: 58%

Nuclear DNA content as an indicator of inflorescence colour stability of in vitro propagated solid and chimera mutants of chrysanthemum

Miler

Kulus

Śliwińska

2020

Plant Cell Tiss Organ Cult

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In chrysanthemum, breeders seek for desirable characteristics of the inflorescence, which can first be established once the plant is mature. The present study aims to determine whether measurement of DNA content can be useful in the detection of somaclonal variants and/or separation of chimera components in chrysanthemum at the early in vitro multiplication stage. Eleven Chrysanthemum × morifolium (Ramat.) Hemsl. cultivars of the Lady group (a mother cultivar and ten of its radiomutants obtained by X-ray- or γ-irradiation; solid and periclinal chimeras) were propagated in vitro. Single-node explants were cultured in Murashige and Skoog (MS) medium, either without plant growth regulators (PGRs) or supplemented with 6-benzyladenine (BA) and indole-3-acetic acid (IAA). The nuclear DNA content was measured by flow cytometry (FCM) in the shoots produced in vitro. After acclimatization and growth of the plants in a glasshouse, inflorescence colour was recorded. The addition of PGRs to the medium almost doubled the mean number of shoots produced in vitro per explant, but caused a change in inflorescence colour of all (‘Lady Apricot’; periclinal chimera) or part of the plants (‘Lady Amber’; solid mutant and ‘Lady Salmon’; periclinal chimera). All radiomutants contained less DNA than the mother cultivar ‘Richmond’. There were significant differences in DNA content between plants of the same cultivar grown in media with or without PGRs for ‘Lady Apricot’ and ‘Lady Salmon’, but no phenotype alternation occurred in chrysanthemums produced in PGR-free medium compared to the original cultivars. Conversely, in medium with PGRs, chimeras produced flowers different from the original colour. In all except one cultivar (‘Lady Amber’; solid mutant) a lack of differences in genome size between plants grown in either medium coincided with a stable inflorescence colour. The occurrence of some plants of ‘Lady Amber’ with different inflorescence colour may be due to small DNA changes, undetectable by FCM. It can be concluded that FCM analysis of DNA content in young plantlets can be indicative of the stability of inflorescence colour in chrysanthemum, especially chimeric cultivars, and for mutant detection.

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“…Rose (Rosa hybrida L.) is a familiar ornamental plant that bears beautiful fl owers and has sharp prickles. Rose prickles are derived from epidermal tissues (Canli 2003, Canli & Skirvin 2008, and present in various forms and densities among varieties (Debener & Linde 2009). Kellogg et al (2011) indicated that rose and raspberry prickles are formed by modifi cation of glandular trichomes, and the fact that both glandular trichomes and immature prickles are present on young twigs of Osaka Rose supports this developmental mechanism as well ( Figure 1F).…”

mentioning

confidence: 99%

Slug caterpillars ofParasa lepida(Cramer, 1799) (Lepidoptera: Limacodidae) become stuck on rose prickles

Yamazaki

Nakatani²,

Masumoto

2014

Pan-Pacific Entomologist

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In vitro separation of a rose chimera

Cited by 7 publications

References 22 publications

In Vitro Segregation of Tetraploid and Octoploid Plantlets from Colchicine-induced Ploidy Chimeras in Echinacea purpurea L.

In Vitro Segregation of Tetraploid and Octoploid Plantlets from Colchicine-induced Ploidy Chimeras in Echinacea purpurea L.

Nuclear DNA content as an indicator of inflorescence colour stability of in vitro propagated solid and chimera mutants of chrysanthemum

Slug caterpillars ofParasa lepida(Cramer, 1799) (Lepidoptera: Limacodidae) become stuck on rose prickles

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