Plant regeneration through somatic embryogenesis and rapd analysis of regenerated plants in Tylophora indica (Burm. F. Merrill.)

Abstract

A procedure for the regeneration of complete plantlets of Tylophora indica from cultured leaf callus via somatic embryogenesis is described. Callus induction from leaf explants was on Murashige and Skoog (MS) medium with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D; 0.0±3 mg l 21 ; 0.0±13.56 mM) and kinetin (Kn; 0.01 mg l 21 ; 0.05 mM). The best response for callus induction was obtained on MS medium containing 2 mg l 21 (9.04 mM) 2,4-D and 0.01 mg l 21 (0.05 mM) Kn. After two subcultures on the same medium the embryogenic callus was transferred to MS medium with different concentrations of the cytokinin, 6-benzyladenine (0.5±3 mg l 21 ; 2.22±13.32 mM) and 2-isopentenyladenine (2ip; 0.53 mg l 21 ; 2.46±14.76 mM) along with 0.01 mg l 21 (0.05 mM) indole-3-butyric acid (IBA) for somatic embryo development and maturation. MS medium with 2 mg l 21 (9.84 mM) 2ip produced the maximum number of mature somatic embryos. The mature embryos were bipolar and on transfer to MS basal medium produced complete plantlets. After hardening the regenerants were planted in the Gudalur forests of Western Ghats. Total DNA was extracted from 14 regenerants and the mother plant. Random amplified polymorphic DNA (RAPD) analysis was carried out using 20 arbitrary oligonucleotides. The amplification products were monomorphic among all the plants revealing the genetic homogeneity and true-to-type nature of the regenerants.

RETRACTED ARTICLE: Plant regeneration in Chlorophytum borivilianum Sant. et Fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis

“…A number of molecular markers can be used to assess the genetic fidelity of in vitro derived plants. However random amplified polymorphic DNA (RAPD) is efficient and cheapest among these markers (Jayanthi and Mandal 2001). In the present investigation, culture conditions were established for improved maturation and germination of somatic embryos and plantlet development in C. borivilianum from embryogenic suspension culture.…”

The root: a potential new source of competent cells for high-frequency regeneration in Tylophora indica

“…Over-exploitation and a lack of organized cultivation has led to a rapid decline in the wild population of T. indica (Jayanthi and Mandal 2001). Micropropagation of T. indica by axillary shoot induction and adventitious shoot production (Sharma and Chandel 1992) and callusmediated somatic embryogenesis from leaf (Jayanthi and Mandal 2001) and stem (Rao et al 1970;Rao and Narayanaswamy 1972) explants have been reported.…”

In Vitro Strategies for the Conservation of Some Medicinal and Horticultural Climbers

“…In another study, Jayanthi and Mandal ( 2001 ) obtained somatic embryos from leaf-derived callus. For callus induction MS medium supplemented with different concentrations of 2, 4-D (2.26-13.56 μM) and 0.05 μM Kn was used.…”