&amp;lt;i&amp;gt;In Vitro&amp;lt;/i&amp;gt; Regeneration of &amp;lt;i&amp;gt;Curcuma caesia&amp;lt;/i&amp;gt; Plantlets from Basal Part and via Somatic Embryogenesis

Zuraida, A. R.; Izzati, Kamaruddin Fatin Liyana; Nazreena, O Ayu; Radziah, C. M. Z. Che; Asyikin, Sheikh Ghadzi Siti Nur; Subramaniam, Sreeramanan

doi:10.4236/abb.2014.54044

Cited by 8 publications

(4 citation statements)

References 18 publications

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“…All the successful induction of callus in this study were obtained in 2, 4-D + BAP supplemented MS media. 2, 4-D alone failed to induce calluses at all the concentration tested (0.5, 1….3 mg/L) which was in line with the report of Zuraida et al, (2014) in C. caesia and also with the finding of Miachir et al, (2004) and Tuan et al, (2011) in C. zedoaria, however, in contradiction to the finding of Saensouk, (2011) were similar and differed from all others (p <0.05). Equal and higher concentration 2,4-D + BAP of 5 mg/L each, responded well (40.00%) to callus induction in contrast to the finding of Taha et al, (2013) who reported higher concentration of 2,4-D up to 4 mg/L in combination with 0.2 mg/L BAP to have performed less in the in vitro culture of Zingiberofficinale using leaves explants.…”

Section: Resultssupporting

confidence: 85%

“…The earliest result was obtained in the 5th week of culture in 2,4-D 0.5 mg/l +BAP 0.1 mg/L, and 2,4-D (0.5 mg/L) + KIN (0.1 mg/L) combinations followed by 2,4-D and BAP (5.0 mg/L each) in the 6th week and 2,4-D (1.0 mg/L) + BAP 0.5 mg/L) and IAA (2.0 mg/L) + BAP (1.0 mg/L) in the 8th week of inoculation. All the responses in term of a number of days from a culture obtained in this study were earlier than what was obtained from C. caesia inoculated on woody plant medium supplemented with 2.0 mg/L 2,4-D + 5.0 mg/L BAP (20%) as reported by Zuraida et al,(2014) where the best callus (20%) was achieved after 70 days of culture.…”

Section: Resultssupporting

confidence: 46%

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Sterilization procedure and callus regeneration in black turmeric (Curcuma caesia)

Abubakar

Pudake

2019

ASD

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Sterilization procedure, media composition, explants selection and control of physical environment are critical for successful cultures and callus induction with surface sterilization being very challenging in most plants. Five different sterilization methods were evaluated to come up with the best for subsequent use to establish an in vitro regeneration method for the induction of callus in Curcuma caesia using excised leaf and rhizome explants. Murashige and Skoog (MS) media supplemented with various concentration of 2,4-Dichlorophenoxy acetic acid (2,4-D)/Indole-3-acetic acid (IAA) (0.5- 5.0mg/L), singly or in combination with Benzyl aminopurine (BAP)/Kinetin (KIN) (0.1-5.0mg/L), 0.3% sucrose and 0.08% agar were used. The result of the sterilization procedures showed 15% NaHClO3 (5min) + 70% Ethanol (30s) + 0.1% HgCl2 (5min) to be the most effective in controlling contamination in C. caesia among all the treatments tested. The response to callus induction was found to depend on the type of explants used and growth regulators combination. Leaf explants gave the highest percentage of callus induction. Highest percentage of callus induction (66.70%) was obtained in the growth regulator combination of 2, 4-D (0.5mg/L) + BAP (0.1mg/L) and least (14.29%) in IAA (2.0mg/L) + BAP (0.5mg/L). Equal and higher concentration of 2, 4-D + BAP of 5.0mg/L each also provided better result (40.00%). No callus was obtained in all the single concentration of 2, 4-D used.

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Section: Resultssupporting

confidence: 85%

Section: Resultssupporting

confidence: 46%

Sterilization procedure and callus regeneration in black turmeric (Curcuma caesia)

Abubakar

Pudake

2019

ASD

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“…Therefore, somatic embryos have potential applications for large-scale production of turmeric plants. Zuraida et al (2014) showed that a culture medium supplemented with 5 mg/l BAP and 2 mg/l 2,4-D enhanced callus induction after 70 days of culture, and that somatic embryogenesis was most successful with MS medium supplemented with 6.0 g/l agar, 5 mg/l BAP and 0.2 mg/l 2,4-D, obtaining 53% of calli differentiation into green somatic embryos and consequently a large number of plantlets. However, since callus formation causes somatic variation and the regenerated plants are not genetically uniform, in vitro screening is necessary.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Propagación in vitro de cúrcuma (Curcuma longa L.) mediante embriogénesis somática directa con referencia a tipos de explantes y reguladores de crecimiento vegetal: una revisión

Upendri

Seran

2021

Ciencias Agronómicas

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La cúrcuma (Curcuma longa L.) es una importante planta medicinal perteneciente a la familia Zingiberaceae. Es una planta triploide estéril, pero es una planta herbácea vital principalmente debido a sus rizomas subterráneos que se utilizan para diversos fines debido a sus propiedades físicas y químicas. La cúrcuma se propaga vegetativamente a través de los rizomas, sin embargo, la tasa de multiplicación es baja. Por otro lado, las técnicas de propagación in vitro se están utilizando con éxito para la propagación masiva de especies de Zingiber directa o indirectamente a través de organogénesis o embriogénesis somática. La regeneración directa de plantas es el método deseado para regenerar plantas genéticamente estables. En la embriogénesis somática directa, las células somáticas se diferencian directamente en embriones somáticos que tienen estructuras bipolares. Los estudios sobre la embriogénesis somática directa de la cúrcuma son muy limitados, por lo tanto, esta revisión resume la comprensión actual de los diferentes aspectos de la embriogénesis somática de la cúrcuma y los principales factores reguladores que la afectan.

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“…Genotype and source of explants would influence the production of embryogenic calli and also the regenerated plantlets [20]. Many reports on successful embryogenic callus induction in many plant species using 2,4-D and BAP hormone have been reported [21]- [24].…”

Section: Introductionmentioning

confidence: 99%

Embryogenic Callus Induction of Aquilaira Malaccensis Lam . a nd Aquilaria Subintegra Ding Hou

Salam*¹,

Awal²,

Abdullah³

2019

IJEAT

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Aquilaria malaccensis Lam. and Aquilaria subintegra Ding Hou belong to the family of Thymelaeaceae which is commonly known as gaharu or agarwood. It is a commercially important tree and identified as a potential aromatic plant. The overwhelming responses in the lodging sector reduce gaharu species in the forest. Mass propagation through plant tissue culture technology will substitute this problem. The present study was conducted to investigate the embryogenic callus induction between these two species. The most optimum sterilization method for both species was sodium hypochlorite 5.0% which gave the highest percentage of aseptic culture (95%) with the absence of tissue browning. The leaves of both species were cultured on Murashige and Skoog, (1962) (MS) media supplemented with combination of various concentrations of 6-benzylaminopurine (BAP) (0.5, 1.0, 2.0 and 2.5 mg/L) and 2,4-dichlorophenoxyacetic acid (2, 4-D) (0.5, 1.0, 1.5 and 2.0 mg/L) and kept under dark condition. The explants produced embryogenic, white and compact callus at the end cut of the explants after two weeks of culture in all treatments. The highest frequency of embryogenic callus formation was observed in explants cultured on 2.0 mg/L BAP and 0.5 mg/L 2,4-D for both species. From the present study, the optimum sterilization technique and embryogenic callus induction for A. malaccensis Lam. and A. subintegra were established.

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<i>In Vitro</i> Regeneration of <i>Curcuma caesia</i> Plantlets from Basal Part and via Somatic Embryogenesis

Abstract: Abstract

Cited by 8 publications

References 18 publications

Sterilization procedure and callus regeneration in black turmeric (Curcuma caesia)

Sterilization procedure and callus regeneration in black turmeric (Curcuma caesia)

Propagación in vitro de cúrcuma (Curcuma longa L.) mediante embriogénesis somática directa con referencia a tipos de explantes y reguladores de crecimiento vegetal: una revisión

Embryogenic Callus Induction of Aquilaira Malaccensis Lam . a nd Aquilaria Subintegra Ding Hou

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