Black spot of rose is distributed throughout the world and is the most serious disease of roses (Rosa spp.) in the outdoor landscape. Resistance breeding has been frustrated by the occurrence of races of the causal pathogen Diplocarpon rosae. Races from Germany, North America and the UK have been characterized and maintained in a pathogenic state. However, these races were characterized using independent sets of host genotypes and are referenced using different nomenclatures. In the present study, a total of 15 D. rosae isolates from these locations, as well as Belgium and Italy, were inoculated to a common set of 15 rose cultivars in replicated, detached leaf trials. Baby Love TM (cv. Scrivluv) was resistant to all isolates except for one originating from the UK. The rose cultivars Mrs Doreen Pike (Ausdor) and Hansa were resistant to all isolates except for one originating from Minnesota, USA. No rose genotype was universally susceptible. A total of 11 pathogenic races were differentiated based on their unique host ranges and were assigned an international race nomenclature. Nine cultivars are proposed as the first standard set of differential genotypes for characterization of D. rosae races.

SummaryColonies of Diplocarpon rosae derived from single conidia were isolated on malt extract agar, multiplied (at 23°C) and stored (at −20°C) on surface‐sterilised leaf discs of a universally susceptible rose, ‘Frensham’. The resistance of 16 species and cultivars of Rosa to different isolates of D. rosae was assessed using surface‐sterilised leaf discs. Four pathotypes of D. rosae were distinguished on the basis of host range. One species and one hybrid were resistant to all pathotypes. Two species and two cultivars were susceptible to all pathotypes. Four species and six cultivars were interpreted as having vertical resistance because they were strongly resistant to some but not all pathotypes. Only species and hybrids of the section Cinnamomeae were resistant to the pathotype identified as CW1 whereas only roses of other origins were resistant to the pathotype DA2.

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High yields of protoplasts were isolated from embryogenic suspension cultures of Rosa persica × xanthina

The gibberellins A(1), A(3), A(5), A(8), A(19), A(20), and A(29) were identified in vegetative shoot tips of Rosa canina by comparing their mass spectra and Kovats retention indices with those of standards. Most wild roses have a short flowering season of 2-4 weeks in spring, whereas most modern cultivars flower recurrently. 'Félicité et Perpétue' is a short-season hybrid from a cross between a wild rose and a recurrent-flowering rose, whereas its sport, 'Little White Pet,' flowers recurrently. The concentrations of gibberellins (GAs) were measured in shoot apices of both cultivars. In March (before floral initiation in spring) the concentrations of GA(1) and GA(3) were respectively threefold and twofold higher in 'Félicité et Perpétue' than in 'Little White Pet.' In April (after floral initiation) the concentrations of both gibberellins were substantially greater than in March, and concentrations of GA(1) and GA(3) were, respectively, 17-fold and 12-fold greater in 'Félicité et Perpétue' than in 'Little White Pet.' It is postulated that, in 'Félicité et Perpétue,' floral initiation occurs when concentrations of GAs are low and is inhibited when concentrations of GAs are high, whereas in 'Little White Pet' concentrations of GAs remain at permissive levels throughout the growing season. Applications of GA(1) and GA(3) to axillary shoots in March inhibited floral development in 'Félicité et Perpétue' but not in 'Little White Pet.' This suggests that the combined concentration of exogenous and endogenous gibberellins might have been raised to inhibitory levels in the former but not in the latter cultivar.

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Plantlets of a diploid species, Rosa wichuraiana, were cultured in vitro in liquid medium and treated with spindle inhibitors or tritiated thymidine . Cytological studies were then carried out on cells of root tips which were excised after the treatments . Exposure to 0 .05 colchicine gave complete spindle arrest with less toxicity than 0 .1% colchicine . Solutions of colcemid gave incomplete spindle arrest even at highly toxic concentrations . After a 0 .5 h pulse of 3H thymidine, the periodicity in the curve of percentage of labelled mitoses against time indicated a cell-cycle-time of 10 h . The highest frequency of tetraploid cells was obtained after exposure to 0 .05% colchicine for 12 h and it is suggested that colchicine prolonged the cell-cycle-time by 2 h . Seven days after a 12 h exposure to 0 .05% colchicine, 32.7% of cells in the root meristem were tetraploid and it is anticipated that a satisfactory yield of non-chimeral tetraploids could be regenerated from such tissues .