A protoplast to plant system in roses

Matthews, D.; Mottley, J.; Horan, I.; Roberts, A. V.

doi:10.1007/bf00033473

Cited by 53 publications

(18 citation statements)

References 18 publications

(25 reference statements)

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“…Although plant regeneration (through a callus phase or not) had already been reported in Rosa from stem segments (Yalles & Boxus, 1978 ;Lloyd et al ., 1988 ;Rout et al ., 1991 ;Matthews et al ., 1991), leaf (Lloyd et al ., 1988;Leffering & Kok, 1990 ;de Wit et al ., 1990;Rout et al ., 1991), root fragments (Lloyd et al ., 1988 ;Matthews et al ., 1991), zygotic embryos (Burger et al ., 1990) and stamen filaments (Noriega & Sondahl, 1991), this is to our knowledge the first time that such direct or indirect regeneration results have been reported in this genus with isolated embryos, petals, sepals, anthers, receptacles and ovules . The observed differences in frequency of callus development according to the floral parts used or to the development stages of zygotic embryos and floral parts confirm the importance, in a regeneration program, of the donor tissue (Evans, 1989 ;Compton & Veilleux, 1991), and its age (Burger et al ., 1990) .…”

Section: Discussionmentioning

confidence: 99%

A comparison of the somaclonal variation level of Rosa hybrida L. cv Meirutral plants regenerated from callus or direct induction from different vegetative and embryonic tissues

Aréne¹,

Pellegrino²,

Gudin³

1993

Euphytica

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Flowering plants of Rosa hybrida L . cv Meirutral have been obtained either from direct regeneration of adventitious shoots on leaf and root fragments, or through organogenesis and somatic embryogenesis on calli derived from anther, ovule, petal, sepal, receptacle, leaf, stem internode, root and zygotic embryo tissues . The calli derived from floral parts exhibited rhizogenesis . In this case direct induction of adventitious shoots from selected roots had to be performed in order to generate plants . A histological study of the morphogenetic calli was carried out . The plants regenerated directly and those regenerated from calli of leaf, stem internode, root and zygotic embryo tissues, together with reference plants propagated by cuttings, were compared on a phenotypic basis by taking into account petal number, form and colour, and plant growth habit . From these observations, it can be concluded that directly regenerated plants are as stable as reference plants while plants regenerated from callus are unstable, especially those derived from zygotic embryo tissues .

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Section: Discussionmentioning

confidence: 99%

A comparison of the somaclonal variation level of Rosa hybrida L. cv Meirutral plants regenerated from callus or direct induction from different vegetative and embryonic tissues

Aréne¹,

Pellegrino²,

Gudin³

1993

Euphytica

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“…The mixture was left undisturbed for 5 min and then gradually diluted with 2 ml of filter-sterilised protoplast culture medium. This consisted of KM8p medium (Kao & Michayluk, 1975) modified after Matthews et al (1991) to include glucose at the reduced concentration of 34.2 g l −1 , mannitol at the increased concentration of 45 g l −1 , 2,4-D (4.5 μM), 1-naphthaleneacetic acid (NAA, 5.4 μM) and BAP (2.2 μM). For each fusion combination, controls were prepared of homofused protoplasts of both parental types.…”

Section: Fusion and Culture Of Protoplastsmentioning

confidence: 99%

“…Apart from one Rosa × Fragaria hybrid, reputedly raised in the Netherlands by Interplant BV, attempts to cross Rosa with other genera of the Rosaceae have been unsuccessful (Roberts & Lewis, 1996). There are no reports of somatic hybridisation in Rosa but plants have been regenerated from protoplasts of callus-derived cell suspensions (Matthews et al, 1991;Schum et al, 2001;Kim et al, 2003) and roots have been regenerated from leaf mesophyll protoplasts (Marchant et al, 1997).…”

Section: Introductionmentioning

confidence: 96%

Cell lines and plants obtained after protoplast fusions of Rosa+Rosa, Rosa+Prunus and Rosa+Rubus

et al. 2005

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Protoplasts of three Rosa cultivars were fused with each other, with protoplasts of Prunus 'Colt' and with protoplasts of Rubus laciniatus, using polyethylene glycol 4000 as a fusogen. Protoplasts of Prunus were incapable of cell division and those of Rosa and Rubus were disabled by treatments with metabolic inhibitors, either iodoacetate (IOA) or rhodamine 6-G (R6G). Parental protoplasts were then fused in combinations that required complementation for their survival. RAPD analysis of 41 fusion-derived cell lines showed that two lines resulting from fusions of Rosa+Rosa and one from a fusion of Rosa+Prunus, contained some DNA markers from both fusion partners. The others contained markers of only one fusion parent. This showed that after protoplast fusion, the heterokaryons did not develop into cell lines with stable hybrid nuclei. Plants regenerated from cell lines derived from Rosa+Prunus and Rosa+Rubus fusions contained DNA markers of only Rosa and their DNA amounts were no greater than that of the Rosa parent. However, they differed morphologically from the Rosa parent to a remarkable degree, possibly because they inherited undetected genes of Prunus or Rubus, or because they were somaclonal variants of the Rosa parent. Alternative strategies for the production of somatic hybrids are discussed.

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“…In the case of floricultural crops, reports of protoplast-to-plant systems have accumulated for Petunia (reviewed in Sink 1991), which belongs to the Solanaceae family, and further investigations based on the protoplast-to-plant system have been actively carried out, especially on the symmetric/asymmetric somatic hybridization for the improvement of floricultural crops (Power et al 1978(Power et al , 1979(Power et al , 1980Schnabelrauch et al 1985;Taguchi et al 1993) and for the analysis of genomic rearrangements (Clark et al 1986;Bonnett and Glimelius 1990;Hinnisdaels et al 1991). Though several studies have succeeded in regenerating commercially important floricultural crops from protoplasts, such as Dianthus (reviewed in Nakano and Mii 1995), Rosa (Matthews et al 1991), Chrysanthemum (Lindsay and Ledger 1993), and Iris (Shimizu et al 1996), reports of successful somatic hybridization have, to the best of our knowledge, been limited (Shimizu et al 1999;reviewed in Nakano et al 2001).…”

Section: Introductionmentioning

confidence: 98%

Production of fertile somatic hybrid plants between Oriental hybrid lily and Lilium�formolongi

Horita

Morohashi

Komai

2003

Planta

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Somatic hybridizations via electrofusion were performed in combinations of Oriental hybrid lilies (cvs. Acapulco and Shirotae) and Liliumxformolongi hort. (cv. Hakucho). Electrofusion-treated protoplasts divided only under nurse culture. The divided protoplasts grew into calli on the culture medium containing picloram, and the calli were then transferred to the hormone-free culture medium for induction of plant regeneration. The regenerants were transferred to a greenhouse, and were grown until the flower stage. In the fusion combinations of Acapulco + Hakucho and Shirotae + Hakucho, four regenerants apparently showed different morphological features compared with their parents. The results of molecular analyses by means of cleaved amplified polymorphic sequences markers and flow cytometry confirmed that these regenerants were somatic hybrid plants. Furthermore, we examined the stability of the morphological features of the hybrids in the next generations. This is the first report to describe the successful realization of Lilium somatic hybridization via protoplast fusion.

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A protoplast to plant system in roses

Abstract: High yields of protoplasts were isolated from embryogenic suspension cultures of Rosa persica × xanthina

Cited by 53 publications

References 18 publications

A comparison of the somaclonal variation level of Rosa hybrida L. cv Meirutral plants regenerated from callus or direct induction from different vegetative and embryonic tissues

A comparison of the somaclonal variation level of Rosa hybrida L. cv Meirutral plants regenerated from callus or direct induction from different vegetative and embryonic tissues

Cell lines and plants obtained after protoplast fusions of Rosa+Rosa, Rosa+Prunus and Rosa+Rubus

Production of fertile somatic hybrid plants between Oriental hybrid lily and Lilium�formolongi

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