Production of fertile somatic hybrid plants between Oriental hybrid lily and Lilium�formolongi

Horita, Mitsugu; Morohashi, Hitomi; Komai, Fuminori

doi:10.1007/s00425-003-1020-9

Cited by 24 publications

(6 citation statements)

References 26 publications

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“…Results of SRAP similarity add weight to this fi nding by demonstrating strong genetic affi nity of primary hybrids to the maternal genotypes. A greater maternal contribution has been demonstrated in many hybrids derived from parental types of mixed ploidy ( Lashermes et al, 2000 ;Haw, 2001 ;Horita et al, 2003 ;Lim et al, 2003 ;Inomata, 2006 ).…”

Section: Results and Applicationsmentioning

confidence: 99%

Sequence‐related amplified polymorphism (SRAP) markers: A potential resource for studies in plant molecular biology¹

2014

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In the past few decades, many investigations in the field of plant biology have employed selectively neutral, multilocus, dominant markers such as inter-simple sequence repeat (ISSR), random-amplified polymorphic DNA (RAPD), and amplified fragment length polymorphism (AFLP) to address hypotheses at lower taxonomic levels. More recently, sequence-related amplified polymorphism (SRAP) markers have been developed, which are used to amplify coding regions of DNA with primers targeting open reading frames. These markers have proven to be robust and highly variable, on par with AFLP, and are attained through a significantly less technically demanding process. SRAP markers have been used primarily for agronomic and horticultural purposes, developing quantitative trait loci in advanced hybrids and assessing genetic diversity of large germplasm collections. Here, we suggest that SRAP markers should be employed for research addressing hypotheses in plant systematics, biogeography, conservation, ecology, and beyond. We provide an overview of the SRAP literature to date, review descriptive statistics of SRAP markers in a subset of 171 publications, and present relevant case studies to demonstrate the applicability of SRAP markers to the diverse field of plant biology. Results of these selected works indicate that SRAP markers have the potential to enhance the current suite of molecular tools in a diversity of fields by providing an easy-to-use, highly variable marker with inherent biological significance.

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Section: Results and Applicationsmentioning

confidence: 99%

Sequence‐related amplified polymorphism (SRAP) markers: A potential resource for studies in plant molecular biology¹

2014

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“…Jeschke et al (2003) and Wetzel et al (1999) indicated that a major problem with assessing natural hybridization in pigweeds is the ability to identify such hybrids. Jeschke et al (2003) suggested that genome size could be used to identify hybrids between A. hybridus and A. tuberculatus Using flow cytometry to detect hybrid plants has become common practice (Barre et al, 1998; Williams et al, 2002; Sisko et al, 2003; Nimura et al, 2003; Horita et al, 2003). Critical to the usefulness of genome size in identifying hybrids is the DNA content of the parental types.…”

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confidence: 99%

Genome Size Analysis of Weedy Amaranthus Species

et al. 2005

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is the agricultural land of the Midwest. Given the intense farming practices used and the nearly constant changes Weedy Amaranthus species pose a serious threat to agriculture.in Best Management Practices, agrichemicals used, etc., One of the areas that causes the greatest concern is development of herbicide resistance and subsequent transfer of herbicide resistance the landscape is in a continual state of flux. If interspegenes among the species. To determine the potential impact of inter-cific hybridization does occur in Amaranthus, introgresspecific hybridization, one must first be able to detect such hybrids.sive hybridization could be very important in the evolu-To determine if genome size could be useful in the detection of intertion and adaptation of these weedy species. specific hybrids among the weedy Amaranthus species, the genome Murray (1940) reported that hybrids could be made sizes of the weedy Amaranthus species sympatric in Illinois were anaamong various monoecious and dioecious pigweed spelyzed. In a series of experiments, the genome sizes of these species cies. Wetzel et al. (1999) and Franssen et al. (2001) rewere determined by flow cytometric analysis. A significant variation ported the production of interspecific hybrids between was observed with respect to nuclear DNA content in the eight species two dioecious pigweed species. Tranel et al. (2002) reexamined. The genome sizes ranged from ≈0.95 pg in A. palmeri to ported interspecific hybridization between a monoe-≈1.4 pg in A. tuberculatus. Overlap of genome sizes among the species does exist; however, due to the reproductive biology of the species, cious and a dioecious pigweed species. In all the above this overlap does not preclude the detection of interspecific hybrids.reports, the hybrids were made under controlled, iso-Any dioecious weedy Amaranthus plant in Illinois that has a genome lated conditions. Trucco et al. (2005) demonstrated insize between 1.3 pg and 1.1 pg is probably a hybrid. Thus, the potential terspecific hybrids between A. hybridus and A. tubercufor genome size determination to reveal interspecific hybrids among latus under field conditions. Thus, it is possible that weedy Amaranthus species has been demonstrated. hybrids between pigweeds could occur naturally in agronomic fields. size to be 5.35 pg per 2C nucleus. Given the almost iden-MH 262 MH 262 Maricopa, AZ tical estimates of DNA, all the weed populations were con-MH 277 MH 277 Beckham, OK MH 254 Brazos, TX verted using 5.35 pg per 2C nucleus for W22 to maintain A. albus PT 66 PT 66 Champaign Co., IL consistency with other data published using W22 as an PT 106 PT 106 Morgan Co., IL internal standard. MH 40 MH 40 Dona Ana, NM MH 49 MH 49 Stoneville, MS The genome size of the pigweed species ranged from MH 55 MH 55 Centre Co., PA 0.96 to 1.21 pg per 2C nucleus in experiment 1 and 0.95 to A. blitoides MH 215 MH 215 Whitman Co., WA 1.23 pg per 2C nucleus in experiment 2 (Table 2). In MH 219 Franklin Co., MD MH 206 Harrow, Ontario both experiments, species were signif...

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“…Within ornamentals, good results were obtained in Liliaceae (Saito and Nakano 2002;Horita et al 2003). Asymmetric protoplast fusion efficiently transmits diverse features such as disease resistance (Jarl et al 1999;Kinoshita and Mori 2001;Collonnier et al 2003), fatty acid composition and salt tolerance (Waara and Glimelius 1995).…”

Section: Introductionmentioning

confidence: 96%

Effect of enzyme concentrations on protoplast isolation and protoplast culture of Spathiphyllum and Anthurium

Duquenne

Eeckhaut

Werbrouck

et al. 2007

Plant Cell Tiss Organ Cult

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Vital protoplasts from Spathiphyllum wallisii ‘Alain’ and Anthurium scherzerianum ‘238’ were isolated from both somatic embryos and leaves. The highest yields were obtained when 1.5% cellulase, 0.5% macerase and 0.5% driselase were used for Spathiphyllum wallisii leaves and 0.5% cellulase, 0.3% macerase and 0.5% driselase for Anthurium scherzerianum embryos. About 1 × 106 protoplasts g−1 and 1 × 105 protoplasts g−1 could be isolated from leaves and embryos, respectively. For protoplast fusion Spathiphyllum wallisii ‘Alain’ and Anthurium scherzerianum ‘238’ were mixed in a 1:1 ratio in a fusion solution containing 1 mM CaCl2·2H2O, 1 mM MES and 0.5 M mannitol. Fusion was performed by protoplast alignment under 500 V cm−1 alternating current for 60 s and subsequent generation of two pulses of 4500 V cm−1 direct current during 50 μs. Development until colony stage was achieved using agarose beads for protoplast culture

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Production of fertile somatic hybrid plants between Oriental hybrid lily and Lilium�formolongi

Cited by 24 publications

References 26 publications

Sequence‐related amplified polymorphism (SRAP) markers: A potential resource for studies in plant molecular biology¹

Sequence‐related amplified polymorphism (SRAP) markers: A potential resource for studies in plant molecular biology¹

Genome Size Analysis of Weedy Amaranthus Species

Effect of enzyme concentrations on protoplast isolation and protoplast culture of Spathiphyllum and Anthurium

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Production of fertile somatic hybrid plants between Oriental hybrid lily and Lilium�formolongi

Cited by 24 publications

References 26 publications

Sequence‐related amplified polymorphism (SRAP) markers: A potential resource for studies in plant molecular biology1

Sequence‐related amplified polymorphism (SRAP) markers: A potential resource for studies in plant molecular biology1

Genome Size Analysis of Weedy Amaranthus Species

Effect of enzyme concentrations on protoplast isolation and protoplast culture of Spathiphyllum and Anthurium

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Product

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Sequence‐related amplified polymorphism (SRAP) markers: A potential resource for studies in plant molecular biology¹

Sequence‐related amplified polymorphism (SRAP) markers: A potential resource for studies in plant molecular biology¹