In Vitro Hardening of Cultured Cauliflower and Chrysanthemum Plantlets to Humidity

Enhancement of callus induction and regeneration efficiency from embryo cultures of Datura stramonium by adjusting carbon sources and concentrations

“…Our study indicated that the most callus fresh weight was observed at 2% lactose. This observation confirms data of Short and Warburton, (1987) The results of the present study showed that the performance of shoot culture of D. stramonium is affected by both the type and concentration of sugar in the culture medium. In general, glucose was better for inducing shoot proliferation than other carbon sources.…”

The effect of sucrose on the development of hybrid walnut microcuttings (Juglans nigra x Juglans regia). Consequences on their survival during acclimatization

“…The assimilation rate observed on plantlets grown on a medium without sucrose was similar to that of seedlings (Short et al, 1987). In hybrid walnut, the absence of sucrose in the rootdevelopment medium induced a low rooting percentage, which is a real problem.…”

Acclimatization strategies for micropropagated plants

“…They often have reduced or absent epicuticular or cuticular wax, which can lack the characteristic crystalline structure, or differ in chemical composition from that of control plants [ 4,5,23,25,28,30,57,59,60,63,64,73]. In vitro leaves had a thinner or somewhat collapsed epidermal layer [4,21,73,74] with a clearly defined [13,58] or an absent or limited [4,23,30,31,73] palisade layer, sometimes with obconically-shaped palisade cells [ 13,18] and a loosely organized spongy mesophyll with an increased percentage air space [4,13,18,23,73].…”

“…The objective is to modify the CIP away from the characteristic hydrophytic-type anatomy and promote epicuticular and cuticular wax development, stomatal function and possibly overcome other deficiencies. Lowering the relative humidity in vitro has been done experimentally with varying results using desiccant in or around the culture container, by coating the medium with oily materials, or both [57,64,76], by opening culture containers into low relative humidity atmospheres [2], adjusting culture closures to reduce the relative humidity [57], using special closures that facilitate water loss [24] or by cooling container bottoms [67 ,68]. Generally the relative humidity could not be lowed to less than 80-85% without culture injury [57,76].…”