The effect of sucrose on the development of hybrid walnut microcuttings (Juglans nigra x Juglans regia). Consequences on their survival during acclimatization

Chenevard, D.; Jay‐Allemand, Christian; Gendraud, M.; Frossard, J.S.

doi:10.1051/forest:19950205

Cited by 11 publications

(2 citation statements)

References 10 publications

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“…We found shoot height to be critical. Since leaf number is positively correlated with shoot length, the number of leaves may be a determining factor for the successful greenhouse acclimatization of plum plantlets, as has been reported in hybrid walnut (Junglans nigra Juglans regia) (Chenevard et al 1995).…”

Section: Discussionmentioning

confidence: 96%

Early antibiotic selection and efficient rooting and acclimatization improve the production of transgenic plum plants ( Prunus domestica L.)

2003

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We describe here an improved system for routinely developing transgenic plum plants (Prunus domestica L.) through the use of Agrobacterium tumefaciens. The production of non-transformed "escapes" has been virtually eliminated, and rates of plant establishment in the greenhouse have been dramatically improved. The system is based on the regeneration of shoots from hypocotyls extracted from mature seed. The shoot regeneration medium is Murashige and Skoog (MS) salts and vitamins supplemented with 7.5 microM thidiazuron and 0.25 microM indole-butyric acid. Transferring the explants after co-cultivation to shoot regeneration medium containing 80 mg l(-1) of kanamycin and 300 mg l(-1) of Timentin reduced the total number of regenerated shoots without affecting the transformation rate. Transformation rates using the described system averaged 1.2% of the hypocotyl slices producing transgenic plants, with a range of 0-4.2%. The transgenic shoots rooted at a rate of 90% on half-strength MS salts and vitamins supplemented with 5 microM alpha-naphthaleneacetic acid and 0.01 microM kinetin. Plantlets were transferred to a greenhouse directly from culture tubes with a 90% average survival.

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Section: Discussionmentioning

confidence: 96%

Early antibiotic selection and efficient rooting and acclimatization improve the production of transgenic plum plants ( Prunus domestica L.)

2003

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“…Additionally, it was also determined that the mortality of microshoots with 1 and 2 roots was considerably higher (84.1%) than that registered for those with 3-6 roots (7.6%). Previously, Chenevard et al [61] associated the improvement of survival of microshoots from hybrid J. nigra n • 23 × J. regia to certain morphological characters, as the number of adventitious roots formed. For Mj209xRa hybrid clones a likely relationship between rooting and survival has also been suggested [17].…”

Section: Acclimatizationmentioning

confidence: 99%

Unraveling Factors Affecting Micropropagation of Four Persian Walnut Varieties

Yegizbayeva¹,

García-García

Yausheva³

et al. 2021

Agronomy

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Walnuts are considered recalcitrant to tissue culture, with a great genetic determinism on all stages of micropropagation; while other factors, also with great impact, become more complicated with the reproduction of newly realized varieties. In this research, a holistic approach aimed to determine the influence of genotype and the nutritive formulation throughout the whole cycle of micropropagation of four Persian walnut varieties (Juglans regia L.) was presented. During the in vitro establishment it was determined that besides genotype and culture medium, the effect of collection season and the likely interaction amongst factors had a great influence on the successful introduction of all four genotypes. However, all cultures were affected by a deep decay, being necessary the introduction of ethylenediamine di-2-hydroxyphenyl acetate ferric, as iron source, and Phloroglucinol in both Murashige and Skoog (1962) and the corrected Driver and Kuniyuki (1987) formulations. These modifications allowed the stabilization of cultures, maintaining thereafter a steady quality. Either proliferation, rooting and ex vitro survival of four clones were affected by the culture medium, obtaining the best results with the corrected Driver and Kuniyuki (1987) formulation. Finally, in vitro plants produced from all clones were acclimated with high survival rates (75.9–91.1% for the best culture medium), depending of clone and the culture medium used. Microsatellite analysis showed that micropropagated plants maintained the same genetic profiles of their corresponding donor trees. These results might contribute to deepening of the understanding of factors that determine the success of micropropagation of walnuts, and the extents of its influence; whereas, it sets the basis for the commercial micropropagation of all four clones.

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