“…On the other hand, stem anatomical characteristics showed significant differences, which were mainly related to cell wall lignification and the size of vessel elements. Water accumulation in the plant results in weak organs and tissues with reduced mechanical support and thin cell walls (Donnelly and Tisdall 1993;Jausoro et al 2010). This accumulation may be caused by the nutritional composition of the medium or the cultivation environment, inhibiting cell wall deposition and the formation of collenchyma and sclerenchyma (Donnelly et al 1985) and restricting the development of the vascular system (Donnelly and Tisdall 1993).…”
Section: Absence Of Sucrose Increased Photosynthesismentioning
Pfaffia glomerata (Spreng.) Pedersen is a medicinal species of great interest because it produces the phytoecdysteroid 20-hydroxyecdysone (20E). Generally, because of atypical growing conditions, in vitro propagated plants function less efficiently as autotrophs and have poorly developed morphological structures. This study analyzed the autotrophic potential of P. glomerata propagated in vitro and evaluated the influence that this has on 20E biosynthesis. Physiological and structural parameters of plants subjected to heterotrophic, photomixotrophic and photoautotrophic growth conditions were evaluated. Levels of 20E were measured by HPLC. Plants were acclimatized in a mixture of soil, sand and substrate, in a greenhouse. Conditions that provided higher carbon input led to an increase in plant growth, and the presence of sucrose was critical, in closure systems without a gas permeable membrane, for normal anatomical development of the micropropagated plants. The absence of sucrose increased photosynthesis and conditions that enhanced photoautotrophy induced greater levels of 20E. The increase of 20E levels by the photoautotrophic system offers new prospects for increasing the commercial production of this species, and for studies that could elucidate the biosynthetic pathway of phytoecdysteroids in plants.
“…On the other hand, stem anatomical characteristics showed significant differences, which were mainly related to cell wall lignification and the size of vessel elements. Water accumulation in the plant results in weak organs and tissues with reduced mechanical support and thin cell walls (Donnelly and Tisdall 1993;Jausoro et al 2010). This accumulation may be caused by the nutritional composition of the medium or the cultivation environment, inhibiting cell wall deposition and the formation of collenchyma and sclerenchyma (Donnelly et al 1985) and restricting the development of the vascular system (Donnelly and Tisdall 1993).…”
Section: Absence Of Sucrose Increased Photosynthesismentioning
Pfaffia glomerata (Spreng.) Pedersen is a medicinal species of great interest because it produces the phytoecdysteroid 20-hydroxyecdysone (20E). Generally, because of atypical growing conditions, in vitro propagated plants function less efficiently as autotrophs and have poorly developed morphological structures. This study analyzed the autotrophic potential of P. glomerata propagated in vitro and evaluated the influence that this has on 20E biosynthesis. Physiological and structural parameters of plants subjected to heterotrophic, photomixotrophic and photoautotrophic growth conditions were evaluated. Levels of 20E were measured by HPLC. Plants were acclimatized in a mixture of soil, sand and substrate, in a greenhouse. Conditions that provided higher carbon input led to an increase in plant growth, and the presence of sucrose was critical, in closure systems without a gas permeable membrane, for normal anatomical development of the micropropagated plants. The absence of sucrose increased photosynthesis and conditions that enhanced photoautotrophy induced greater levels of 20E. The increase of 20E levels by the photoautotrophic system offers new prospects for increasing the commercial production of this species, and for studies that could elucidate the biosynthetic pathway of phytoecdysteroids in plants.
“…In addition to plant-related factors, acclimatization success is also related to the container, shading, substrate, leaves and root length [44,45]. In this work we observed factors such as the selection of plants with roots above 2 cm in length and shoots with two or more leaves and a pre-acclimatization stage in germination chambers may have directly contributed to the survival rates during this complex transition phase from the in vitro medium to the ex vitro condition.…”
Jelly palm (Butia capitata) is a species of palm tree endemic to the Brazilian Savannah (Cerrado) Biome, which stands out for its use as food, which has resulted in a predatory exploitation of its natural populations. This study aimed to characterize fruits and diaspores of natural populations of jelly palm (‘Arinos’, ‘Mirabela’ and ‘Serranópolis’), in addition to developing ex situ conservation strategies of the species, through the storage of zygotic embryos at ultra-low temperatures of liquid nitrogen (LN). Initially, fruits, seeds and embryos were evaluated for their morphological characteristics. For conservation, hydrated zygotic embryos were desiccated for up to 12 h in a laminar-flow chamber and immersed directly in LN with different moisture contents. As a result, we observed morphological differences among the studied populations. The population ‘Arinos’ showed more expressive results for most of the evaluated characteristics, such as length, width of fruits and seeds. The cryopreservation of zygotic embryos proved to be efficient for the conservation of the species reaching germination rates between 70 and 86%, when the moisture content of the embryos immersed in LN were between 10 and 14%. The plants that reached the stage of the greenhouse had a rate of more than 80% survival. The variability in the characteristics of fruits and diaspores of the analyzed populations allow for establishing divergent groups for the definition of conservation strategies or genetic improvement. The developed cryopreservation protocol can be considered simple and efficient for the conservation of the genetic variability of the species.
“…standard green house features a balanced atmosphere for adapting plants grown in vitro (Kshitij, 2012). Controlled humidity and temperature are often the most important variables to meet during acclimatization for in vitro plants, which lack structured cell components, particularly hard cuticles to prevent fast evapotranspiration (Donnelly, 2015). Green house installation with poor materials (poly house) has a greater effect on raising temperature than the outside environment, leading the plants to experience rapid evapotranspiration.…”
Section: In Vitro Production Trends From the Year 2016 To 2019mentioning
Plant tissue culture techniques have encountered a lot of obstacles and breakthroughs as a life-giving technology in numerous field of biotechnology research. Tissue culture technology has evolved throughout times in the world, from shoot tip culture to variety creation. Plant tissue culture may be thought of as a collection of techniques/methodologies that make uses distinct rooms and essential facilities.
The Areka tissue culture research laboratory was established with the aim of conducting comprehensive plant biotechnology researches. However, numerous challenges and opportunities have arisen in the course of attempting to conduct tissue culture experiments in the lab. In fact, the lab highlighted several accomplishments in a few key areas while also tracking improvement over time. Therefore, this walk-through review offers an overall picture of the lab in relation to the research plans, status, and trends. As a result, contribute to the provision of baseline information for future study advancement.
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