Somaclonal Variation in Plants Regenerated by Organogenesis From Callus Culture of Strawberry (Fragaria X Ananassa)

Popescu, A.; Isac, V.; Coman, M.; Radulescu, M.

doi:10.17660/actahortic.1997.439.8

Cited by 27 publications

(25 citation statements)

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“…The occurrence of polyploidy and aneuploid changes in long-term callus cultures of strawberry has been reported (Nehra et al, 1991(Nehra et al, , 1992. Additionally, some of the cytological abnormalities observed in long-term callus cultures are passed onto regenerants from such cultures (Nehra et al, 1992;Popescu et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Ex vitro Performance and Genetic Stability of Strawberry Plants Derived from Different in Vitro Propagation Methods

Mohamed

El-Hamed

Omar

et al. 2017

HJSC

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Ex vitro experiment was conducted at the greenhouse and nursery facilities of the Plant Tissue Culture Laboratory of Department of Horticulture, Faculty of Agriculture, Suez Canal University during the period 2009-2012. Strawberry ex vitro growth performance of tissue culture-derived plants from different propagation methods (meristem, direct regeneration and callus-derived plants) were examined with standard runner-propagated mother plants based on morphological traits and genetic analysis using RAPD. Ex vitro growth performance examination showed that leaf morphology and serration patterns were almost similar among plants obtained from meristem, direct or callus regeneration. Tissue culture plants have higher number of runners while standard runner plants were higher in flower production compared with in vitro propagated plants. RAPD analysis indicated that plants of cv. 'Tudla' from the different in vitro propagation methods were genetically stable, while in cv. 'Festival', callus-derived plants deviated from normal genotype. The obtained results confirmed the usefulness of RAPD in determining genetic stability of plants clonally propagated in vitro as well as detecting the somaclonal variants, which might be used further in breeding programs.

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Section: Discussionmentioning

confidence: 99%

Ex vitro Performance and Genetic Stability of Strawberry Plants Derived from Different in Vitro Propagation Methods

Mohamed

El-Hamed

Omar

et al. 2017

HJSC

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“…Very large impact on genetic stability has cultivated in vitro plant genotype [Nehra et al 1994]. Somaclonal variation of strawberry (Fragaria × ananassa) may be induced from callus leaf explants through increased interval between subsequent passages [Popescu et al 1997], gamma irradiation in doses of 5-50 Gy [Kaushal et al 2004] or using high concentration of BAP [Biswas et al 2009]. …”

Section: Genetic Stabilitymentioning

confidence: 99%

“…Results of some studies suggested that, the important factors in somaclonal variation induction in in vitro systems are concentration of auxins in medium and number of passages [Popescu et al 1997, Biswas et al 2009]. …”

Section: Genetic Stabilitymentioning

confidence: 99%

In vitro REGENERATION OF Fragraria PLANTS

Rokosa

Mikiciuk

2017

Acta Sci. Pol. Hortorum Cultus

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Fragaria is one of genus in Rosaceae family. The most popular representative is strawberry (Fragaria × ananassa Duch.) and wild (woodland) strawberry (Fragaria vesca L.), which taste attributes are very attractive for a huge number of consumers around the world. The plants have many beneficial traits, such as low-caloric, high amount of antoxidants and vitamin C, laxative, diuretic, astringent, antidiarrheal and antiseptic properties. Cultivation of Fragaria plants is widespread worldwide with particular emphasis on moderate climate zone, also with use of a plant tissue culture method. This thesis showed and contrasted other studies about Fragaria plants propagation under in vitro conditions. In this method the most often used explants are leaf explants. Very rarely are used seeds. Mainly, the plants are propagated on basal medium of mineral composition by Murashige and Skoog in 8 hour dark and 16 hour light contidions. The most efficient cytokinin used to root induction is indole-3-butyric acid (IBA). The plant acclimatization had varying effectiveness -from a few to several dozen survival percent. During micropropagation of Fragaria plants, somaclonal variation occurs, which is dependent on age culture, frequency of passage and medium composition.

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“…However, different genomes respond differently to the stress-induced variation, which indicates that somaclonal variation also has genotypic components. In callus cultures of strawberry, the genotype and type of explant strongly influenced the occurrence of somaclonal variation [64]. The differences in genetic stability are related to differences in genetic make-up, because some components of the plant genome may become unstable during the culture process, for example the repetitive DNA sequences, which can differ in quality and quantity between plant species [65].…”

Section: Effect Of Genotypementioning

confidence: 99%