In vitro REGENERATION OF Fragraria PLANTS

Abstract

Fragaria is one of genus in Rosaceae family. The most popular representative is strawberry (Fragaria × ananassa Duch.) and wild (woodland) strawberry (Fragaria vesca L.), which taste attributes are very attractive for a huge number of consumers around the world. The plants have many beneficial traits, such as low-caloric, high amount of antoxidants and vitamin C, laxative, diuretic, astringent, antidiarrheal and antiseptic properties. Cultivation of Fragaria plants is widespread worldwide with particular emphasis on moderate climate zone, also with use of a plant tissue culture method. This thesis showed and contrasted other studies about Fragaria plants propagation under in vitro conditions. In this method the most often used explants are leaf explants. Very rarely are used seeds. Mainly, the plants are propagated on basal medium of mineral composition by Murashige and Skoog in 8 hour dark and 16 hour light contidions. The most efficient cytokinin used to root induction is indole-3-butyric acid (IBA). The plant acclimatization had varying effectiveness -from a few to several dozen survival percent. During micropropagation of Fragaria plants, somaclonal variation occurs, which is dependent on age culture, frequency of passage and medium composition.

Optimization of Shoot Regeneration and Application of CRISPR/Cas9 Gene Editing to Cultivated Strawberry (Fragaria×ananassa)

“…The runner segment is not a commonly used explant for strawberry micropropagation (Rokosa and Mikiciuk 2017). This is because most regeneration protocols were developed using in vitro cultured plantlets.…”

“…The application of CRISPR-mediated gene editing to an octoploid strawberry requires dedicate procedures of tissue culture and the shoot regeneration. There have been successful reports of micropropagation from octoploid strawberry (Rokosa and Mikiciuk 2017;Nehra et al 1989;Passey et al 2003). However, regeneration and morphological development in octoploid strawberry are greatly influenced by tissue culture conditions, explant types, and genotypes (Landi and Mezzetti 2006;Rokosa and Mikiciuk 2017;Debnath and Teixeira da Silva 2007;Husaini et al 2011).…”

“…There have been successful reports of micropropagation from octoploid strawberry (Rokosa and Mikiciuk 2017;Nehra et al 1989;Passey et al 2003). However, regeneration and morphological development in octoploid strawberry are greatly influenced by tissue culture conditions, explant types, and genotypes (Landi and Mezzetti 2006;Rokosa and Mikiciuk 2017;Debnath and Teixeira da Silva 2007;Husaini et al 2011). Therefore, it is often necessary to optimize tissue culture conditions for different strawberry varieties.…”

Micropropagation of Stevia rebaudiana plants

“…Such differences can be attributed to the physiological condition of the explant, which is determined by genetic factors. Multiple passages foster mutation (ROKOSA & MIKICIUK, 2017) In this study the plants originated from several-year-old culture -in the CHOTIKADACHANARONG & DHEERANUPATTAN'S ( 2013) study plants could be younger or older (there is no information).…”

Minimal Media Strength for In vitro Conservation of Strawberry (Fragaria ananassa) Cultures

“…This method is not desirable because it produces plants that are genetically different from the parent plant and therefore percentage of seed germination is low, so that they are propagated by vegetative methods. The strawberry plant's infection with some pathogens, especially viral diseases, result in a decrease in production in quantity and quality, so it is preferable to use the tissue culture method to generate plants free from pathogens for commercial production (Rokosa & Mikiciuk, 2017).…”