Ex vitro Performance and Genetic Stability of Strawberry Plants Derived from Different in Vitro Propagation Methods

Abstract

Ex vitro experiment was conducted at the greenhouse and nursery facilities of the Plant Tissue Culture Laboratory of Department of Horticulture, Faculty of Agriculture, Suez Canal University during the period 2009-2012. Strawberry ex vitro growth performance of tissue culture-derived plants from different propagation methods (meristem, direct regeneration and callus-derived plants) were examined with standard runner-propagated mother plants based on morphological traits and genetic analysis using RAPD. Ex vitro growth performance examination showed that leaf morphology and serration patterns were almost similar among plants obtained from meristem, direct or callus regeneration. Tissue culture plants have higher number of runners while standard runner plants were higher in flower production compared with in vitro propagated plants. RAPD analysis indicated that plants of cv. 'Tudla' from the different in vitro propagation methods were genetically stable, while in cv. 'Festival', callus-derived plants deviated from normal genotype. The obtained results confirmed the usefulness of RAPD in determining genetic stability of plants clonally propagated in vitro as well as detecting the somaclonal variants, which might be used further in breeding programs.

Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (Ribes grossularia L.)

“…In order to assess the genetic variability of berry plants, the RAPD and ISSR markers are most frequently used. In several research papers, the use of AFLP, SSR, and SRAP markers was also reported [17,19,21,25,[61][62][63]65,[70][71][72]. AFLP analysis proved to be more reliable and sensitive than other methods not only in our research, but also in the studies of Lanham and Brennan [21] and Costa and co-workers [31].…”

“…Using both marker systems, the lowest genetic variability was detected in Hinnonmaki Rot and the highest in Invicta. Mohamed and co-workers [61] assessed the genetic stability of in vitro-derived plants of several strawberry cultivars using RAPD markers. The study also revealed that the frequency of genetic variation depends on the cultivar studied; plants of Tudla, depending on the culture conditions, showed polymorphism within 2.86-11.77%, while the variability in the cultivar Festival ranged from 10.35 to 30.77%.…”

Questions of Phenotypic Stability and Physiological Status of Strawberry Plants (Fragaria × Ananassa Duch.) During Long Cultivation in Vitro

“…Однако внедрение в производство биотехнологических методов повлекло за собой и новый ряд принципиальных проблем. Достаточно скоро в научной литературе стала появляться информация о том, что у растений, полученных in vitro, периодически наблюдаются аномалии роста и развития [2,3]. В результате возникло предположение о дрейфе генотипа и, как результат, возможной генетической неоднородности получаемого материала [4].…”