Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (Ribes grossularia L.)

Abstract

Gooseberry (Ribes grossularia L.) is a small fruit crop producing valuable fruits, which is constantly gaining importance. In vitro propagation of this species can significantly support the production of virus-free planting material and accelerate the introduction of new cultivars to the market. The aim of presented study was to assess field performance and genetic stability of micropropagated plants (MPs) of four gooseberry cultivars, “Captivator”, “Hinnonmaki Rot”, “Invicta”, and “Resika”. The growth vigor and yield of MPs and plants propagated by standard methods from softwood cuttings (ST) were evaluated in a field experiment. Microscopic observations of the number and length of the stomata of MP and ST plants were carried out. Two DNA-based techniques, amplified fragment length polymorphism (AFLP) and inter simple sequence repeat (ISSR), were used to assess genetic stability of MP plants. For analysis of genetic stability of ST plants, the ISSR technique was applied. For three cultivars, Captivator, Hinnonmaki Rot, and Invicta, the plants’ growth vigor and fruit yield were greater in MP plants than in ST plants. In the case of Resika, most of these parameters were higher in ST plants. Microscopic observations of the stomata indicated a lack of differences in the length between MP and ST plants, while the stomata frequency on leaves of MP plants was higher than that of ST plants. The genetic variability of MP plants, assessed by AFLP, ranged from 0.35% for Hinnonmaki Rot to 2.12% for Resika. The results of ISSR analysis of MP plants showed variability from 0% in the case of Hinnonmaki Rot and Resika to 4% and 8.69% for Captivator and Invicta, respectively. No polymorphism was detected among ST plants of all analyzed gooseberry cultivars.

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

“…It is an efficient, quick, and reproducible technique in which the targets are the DNA fragments located between adjacent microsatellite regions, while the RAPD-PCR targets are random [18]. Wójcik et al [19] used ISSR primers to observe the genetic stability of regenerated plants of Ribes grossularia L. Both techniques are used together to obtain more realistic and accurate results [20][21][22].…”

In vitro propagation for conservation and genetic fidelity of the near threatened Dimocarpus longan plant

“…This observation agrees with that of Mirzaei et al [75] concluded that true-to-typeness of micropropagated olive was cultivar-dependent using partec flow cytometry (FCM) and AFLP analysis. Also, Wójcik et al [76] employed two DNA-based approaches to assess genetic stability of micropropagated plants, amplified fragment length polymorphism (AFLP) and inter simple sequence repeat (ISSR), and found no polymorphism among the analyzed gooseberry cultivars. Other plant species with consistent genetic fidelity in in vitro regenerates include Prunus ulcis [77], Brassica oleracea [78], and Rhodiola imbricate [79].…”

Application and Improvement of In Vitro Culture Systems for Commercial Production of Ornamental, Fruit, and Industrial Plants in Poland

“…Meta-topolin enabled shoot elongation as well as efficient rooting and acclimatization. e ISSR and AFLP analyses showed that the method of in vitro propagation of gooseberry used in their research was suitable for obtaining plants with high genetic fidelity (Wójcik et al, 2021). Application of mT enabled the initiation and propagation of shoots in in vitro cultures of black currant for polyploidization and then for the propagation of the obtained tetraploids (Podwyszyńska & Pluta, 2019).…”