Application and Improvement of In Vitro Culture Systems for Commercial Production of Ornamental, Fruit, and Industrial Plants in Poland

Podwyszyńska, Małgorzata; Orlikowska, T.; Trojak‐Goluch, Anna; Budzyńska, Agnieszka

doi:10.5586/asbp.914

Cited by 10 publications

(8 citation statements)

References 114 publications

(126 reference statements)

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“…Here, a group of researchers consisting of Eleonora Gabryszewska and Małgorzata Podwyszyńska developed protocols (among others, based on SE) that are important for the development of horticulture in Poland. Podwyszyńska et al (2022) described these findings in a separate study. On the other hand, the development of a suspension culture system for gentians at the Department of Plant Conservation Biology, PAS BG-CBDC, brought new knowledge to experimental plant biology.…”

Section: Crop Plantsmentioning

confidence: 81%

Polish Contribution to Global Research on Somatic Embryogenesis

et al. 2022

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Somatic embryogenesis (SE) is a complex process that begins with regaining totipotency in some somatic cells, proceeds through embryo development and maturation, and ends with the formation of a whole plant. Since the first publications on SE in 1958, this regeneration process has been applied to the in vitro propagation of many plant species and has led to the development of some specific model systems. SE has been used to expand our understanding of the cytomorphological, physiological, biochemical, and genetic processes that govern the earliest developmental events in the life of plants. This paper summarizes the achievements of Polish research groups working on SE systems established for several plants (gentians, the tree fern Cyathea delgadii Sternb., and conifers) and three model species ( Arabidopsis thaliana , Medicago sativa , and M. truncatula ). SE systems have used a broad spectrum of experimental approaches involving genomic tools (transcriptomics, proteomics, and chromatin analyses), physiological methods which focus on phytohormones, and cytological techniques. Studies on the experimental models of A. thaliana and Medicago spp. have resulted in the identification of new genetic and epigenetic elements of the complex regulatory network controlling embryogenic induction in plant somatic cells. The protocol developed for ferns has provided a unique and simple system for cytological analysis of early SE events that occur in a single cell of initial explants. Gentian embryogenic suspension cultures have successfully been used in broad biotechnological applications, including plant transformation, protoplast isolation, culture, and fusion. Systems described for coniferous species effectively produced many vigorous somatic seedlings and cost-efficient storage of genotypes during clonal field-testing. The research undertaken by Polish scientists has resulted in developing experimental systems that have enabled significant advances in SE knowledge.

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Section: Crop Plantsmentioning

confidence: 81%

Polish Contribution to Global Research on Somatic Embryogenesis

et al. 2022

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“…Micropropagation is a widely recognized method of vegetative propagation that allows for the production of numerous offspring plants. Podwyszy ńska et al [44] reported that the global annual production of ornamental plants through in vitro cultures has surged from 800 million to 2 billion in the last decade. According to a recent research report, the micropropagation market size is expected to rise by 2030, reaching a value of 2.8 billion USD (about 2.5 billion EUR; report "Micropropagation Market-Global Outlook and Forecast 2022-2027"-https://www.reportlinker.com/p06313600/?utm_source=GNW, accessed on 25 February 2024).…”

Section: Micropropagationmentioning

confidence: 99%

“…According to a recent research report, the micropropagation market size is expected to rise by 2030, reaching a value of 2.8 billion USD (about 2.5 billion EUR; report "Micropropagation Market-Global Outlook and Forecast 2022-2027"-https://www.reportlinker.com/p06313600/?utm_source=GNW, accessed on 25 February 2024). Europe is one of the most prominent geographical segments in the global micropropagation market, with 140 commercial in vitro laboratories for which the production of ornamental plants is pivotal [44].…”

Section: Micropropagationmentioning

confidence: 99%

A Review on Flower Bulb Micropropagation: Challenges and Opportunities

Yasemin,

Beruto

2024

Horticulturae

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This comprehensive review scrutinizes tissue culture and micropropagation methodologies in geophytes, focusing on bulbous plants. The examination encompasses key stages, including somatic embryogenesis, bulb growth, dormancy breaking, and planting. Studies underscore the pivotal role of plant growth regulators (PGRs) in plant regeneration and bulb growth. Bioreactor systems for healthy plant regeneration, rooting methods, acclimatization strategies, and considerations for ex vitro survival are elucidated. The review also delves into somaclonal variation dynamics and acknowledges the burgeoning field of gene editing, particularly Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) studies, as a promising avenue for enhancing valuable compound content in geophytes. In addition to addressing challenges in flower bulb micropropagation, this review briefly highlights emerging opportunities, including the potential integration of artificial intelligence (AI) to optimize culture conditions, predict growth parameters, and enhance efficiency in bulb production. The conclusion emphasizes the necessity of a multifaceted approach integrating biochemistry, physiology, and molecular biology to address existing challenges and improve tissue culture protocols for diverse geophyte species. This review article also intends to highlight how tissue culture techniques could contribute to the development and valorization of flower bulbs in today’s scenario of the ornamental industry.

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“…Furthermore, plants produced in vivo have poor and uneven oil contents and yield [4]. Consequently, the in vitro propagation of roses has played a critical role in the fast multiplication of species and cultivars with desirable characteristics and in the production of healthy plants [6].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Shoot Multiplication and Rooting of ‘Kashan’ and ‘Hervy Azerbaijan’ Damask Rose (Rosa damascena Mill.) Genotypes for Cosmetic and Ornamental Applications

Kaviani,

Deltalab,

Kulus

et al. 2024

Plants

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The damask rose (Rosa damascena Mill.) is an ornamental–medicinal plant from the Rosaceae family, and its aromatic compounds and essential oils are applied globally in the food, cosmetic, and pharmaceutical industries. Due to its economic value, this research aimed to establish a protocol for an efficient, rapid, and cost-effective method for in vitro shoot multiplication and rooting of the R. damascena ‘Kashan’ and ‘Hervy Azerbaijan’ genotypes. Nodal segments (as primary explants) were cultured on the Murashige and Skoog (MS) medium with combinations of various plant growth regulators (PGRs) such as gibberellic acid (GA3), 6-benzylaminopurine (BAP), and indole-3-butyric acid (IBA), as well as a PGR-like substance, phloroglucinol (PG), vitamins such as ascorbic acid (AA), and activated carbon in the form of active charcoal (AC). For the establishment stage, 0.1 mg·L−1 PG, 0.2 mg·L−1 GA3, and 1 mg·L−1 BAP were added to the media. Secondary explants (nodal segments containing axillary buds produced from primary explants) were obtained after 30 days of in vitro culture and transferred to the proliferation media supplemented with different concentrations of BAP (0, 0.5, 1, 1.5, 2, and 2.5 mg·L−1) and GA3 (0, 0.1, 0.2, 0.4, 0.8, and 1 mg·L−1) together with 0.1 mg·L−1 PG and 20 mg·L−1 of AA. The rooting media were augmented with different concentrations of BAP and GA3 with 0.1 mg·L−1 of IBA, PG and 20 mg·L−1 of AA and AC. The results showed that the highest regeneration coefficient (4.29 and 4.28) and the largest number of leaves (23.33–24.33) were obtained in the explants grown on the medium supplemented with 2 mg·L−1 BAP and 0.4 mg·L−1 GA3 for the ‘Kashan’ and ‘Hervy Azerbaijan’ genotypes, respectively. Likewise, this PGR combination provided the shortest time until bud break (approximately 6.5 days) and root emergence (approximately 10 days) in both genotypes. The highest number of shoots (4.78 per explant) and roots (3.96) was achieved in this medium in the ‘Kashan’ rose. Stem and root lengths, as well as stem and root fresh and dry weights, were also analyzed. In most measured traits, the lowest values were found in the PGRs-free control medium. Rooted plantlets were transferred to pots filled with perlite and peat moss in a 2:1 proportion and were acclimatized to ambient greenhouse conditions with a mean 90.12% survival rate. This research contributes significantly to our understanding of Damask rose propagation and has practical implications for the cosmetic and ornamental plant industries. By offering insights into the manipulation of regeneration processes, our study opens up new possibilities for the effective production of high-quality plant material.

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Application and Improvement of In Vitro Culture Systems for Commercial Production of Ornamental, Fruit, and Industrial Plants in Poland

Cited by 10 publications

References 114 publications

Polish Contribution to Global Research on Somatic Embryogenesis

Polish Contribution to Global Research on Somatic Embryogenesis

A Review on Flower Bulb Micropropagation: Challenges and Opportunities

In Vitro Shoot Multiplication and Rooting of ‘Kashan’ and ‘Hervy Azerbaijan’ Damask Rose (Rosa damascena Mill.) Genotypes for Cosmetic and Ornamental Applications

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