Somaclonal Variation in Tissue Culture: A Case Study with Olive

Leva, A. R.; Petruccelli, R.; Rinaldi, Laura

doi:10.5772/50367

Cited by 79 publications

(71 citation statements)

References 121 publications

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“…Similar results were reported using the RAPD technique in Norway spruce (Fourré et al 1997), Japanese black pine (Goto et al 1998), Japanese white oak (Thakur et al 1999), loblolly pine (Tang 2001), almond (Martins et al 2004), hazelnut (Nas et al 2004, pedunculate oak (Valladares et al 2006), cork oak (Fernandes et al 2011), apricot (Soliman 2012), annatto (Siril and Joseph 2013), and seedless lemon (Goswami et al 2013). In contrast, RAPD analysis reported genetic variation in poplar (Ostry et al 1994;Rani et al 1995), white spruce (Isabel et al 1995(Isabel et al , 1996, peach (Hashmi et al 1997), teak (Gangopadhyay et al 2003), Idaho locust (Ngezahayo et al 2006), stone pine (Cuesta et al 2010), and olive (Leva et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Genetic stability of in vitro-propagated plants should be assessed as early as possible, especially in long-living trees such as conifers (Marum et al 2009). At present, different molecular analytical techniques are used to highlight somaclonal variation in tissue culture and regenerants of several plants (Leva et al 2012). Several researchers have used RAPD techniques to investigate genetic variability and have found it very efficient and reliable (Goto et al 1998).…”

Section: Discussionmentioning

confidence: 99%

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Screening RAPD primers to assess clonal fidelity in somatic embryos of Sawara cypress (<i>Chamaecyparis pisifera</i> Sieb. et Zucc.) and field performance of somatic embryo-derived trees

Hosoi

Kuramoto

Maruyama

2015

Plant Biotechnology

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In this study, 100 random amplified polymorphic DNA (RAPD) primers were screened to assess clonal fidelity in somatic embryos of Sawara cypress (Chamaecyparis pisifera Sieb. et Zucc.). Of the 100 primers tested, eight were selected based on their amplification products, which showed clear DNA fragmentation, generating 8.75 bands per primer in average. The amplification products showed no genetic variation among the tested somatic embryos. In addition, the performance of somatic embryo-derived trees was monitored in the field, and their mean height growth was measured until the age of 10 years. Although no phenotypic differences were observed, the somatic embryo-derived trees showed lower growth compared with seedlings.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Screening RAPD primers to assess clonal fidelity in somatic embryos of Sawara cypress (<i>Chamaecyparis pisifera</i> Sieb. et Zucc.) and field performance of somatic embryo-derived trees

Hosoi

Kuramoto

Maruyama

2015

Plant Biotechnology

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“…Similar results were observed by the Escandon et al [15], they reported the change of leaf and flower size of the Brahmi after treatment with Colchicine. Babil et al [28], Leva et al [6], Mujib [29] and Omidbaigi et al [30] also reported the effects of the colchicines on the leaf size of various plants. Many other researchers have reported that the increase of ploidy often causes anatomical and structural changes in the plants [10].…”

Section: Effect Colchicine Treatments On Morphology Of Regenerated Plmentioning

confidence: 99%

“…Also, there is abnormal pigmentation observed in the leaves showing yellowish, pale yellow and colourless leaves (Figure 5c and 5d) and the variations in the leaf shape and size (Figure 5b-5e) as compare to the normal plant regenerated without colchicine treatment. Leva et al [6] suggested that the morphological characters are used for the detection of somaclonal variation. However, these variations were not continued, when recultured on the same regeneration media, hence these variations are the epigenetic somaclonal variations [18].…”

Section: Effect Colchicine Treatments On Morphology Of Regenerated Plmentioning

confidence: 99%

In Vitro Enhancement of Bacoside in Brahmi (Bacopa monnieri) Using Colchicine

Av¹,

Ns²,

Ma³

et al. 2017

J Plant Biochem Physiol

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“…According to Leva et al (2012), the system by which the regeneration is induced, type of tissue, explant source, media components and the duration of the culture cycle are some of the factors that are involved in inducing variation during culture. in vitro CONCLUSIONS RAPD assay could detect genetic changes in micropropagated pineapple so that it can be used as early detection of off-type plant.…”

Section: Shorter Period Of Culture Could Suppress the Somaclonal Varimentioning

confidence: 99%

RAPD Analysis to Detect Somaclonal Variation of Pineapple In Vitro Cultures during Micropropagation

Roostika

Khumaida²,

Ardie³

2016

BIOTROPIA

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Plant off-type becomes a concern in pineapple micropropagation. Reliable methods are needed to detect and to reduce plant off-type. This research was conducted to confirm the occurrence of somaclonal variation during micropropagation of pineapple clone Simadu. The culture period (long-and short-period) and the regeneration methods (direct organogenesis, indirect organogenesis and somatic embryogenesis) were studied to know their contribution in bringing out somaclonal variation. RAPD analysis using 10 primers was performed to confirm genetic variation. The result showed that RAPD assay could be applied as early detection of somaclonal variation of pineapple, where OPA primers were better to be used than OPJ primers. The phenotypic variation occurred in the four year-old pineapple plants and plantlets were due to genetic variation. It has proved that the long-period of cultures is the main contributor of somaclonal variation, while the regeneration method and plant growth regulator could also induce genetic variation. The new cultures showed higher level of similarity. Therefore, it needs a correct strategy to apply micropropagation method of pineapple to minimize plant off-type. It was recommended to avoid the use of long-period culture as mother stock and to apply direct organogenesis method for micropropagation rather than indirect organogenesis and somatic embryogenesis.

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Somaclonal Variation in Tissue Culture: A Case Study with Olive

Cited by 79 publications

References 121 publications

Screening RAPD primers to assess clonal fidelity in somatic embryos of Sawara cypress (<i>Chamaecyparis pisifera</i> Sieb. et Zucc.) and field performance of somatic embryo-derived trees

Screening RAPD primers to assess clonal fidelity in somatic embryos of Sawara cypress (<i>Chamaecyparis pisifera</i> Sieb. et Zucc.) and field performance of somatic embryo-derived trees

In Vitro Enhancement of Bacoside in Brahmi (Bacopa monnieri) Using Colchicine

RAPD Analysis to Detect Somaclonal Variation of Pineapple In Vitro Cultures during Micropropagation

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