In Vitro Enhancement of Bacoside in Brahmi (Bacopa monnieri) Using Colchicine

Av, Kharde; Ns, Chavan; Ma, re; Rh, Autade; Mb, Khetmalas

doi:10.4172/2329-9029.1000172

Cited by 5 publications

(1 citation statement)

References 24 publications

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“…Some features of polyploidy plants include nuclei and larger cell contents; leaves and flowers which grow larger; and it may undergo changes in chemical compounds including an increase or change in the size or proportion of carbohydrates, proteins, vitamins, or alkaloids (As'adah et al, 2016 ;Sivakumar et al, 2017). Based on several studies, it appears that colchicine can cause ploidation of the Ponkan Mandarindan citrus (Dutt et al, 2009), tomato plants (Adelanwa et al, 2011), garlic root (Pharmawati and Waitiani, 2015), maize (Winaryo et al , 2016), and red ginger ) marked by chromosomal changes as well as increasing chromosome number, species change in rose plants (Sarwar and Butt, 2015), regeneration and variation of leaf number on Bacopa monnieri (Kharde et al , 2017) and induce embryonic development (Azmi et al, 2015). Beside colchicines concentrations, the timing of colchicines treatment may also affect polyploidy of plants.…”

Section: Introductionmentioning

confidence: 99%

Response of Pineapple Callus (Ananas comosus Merr.) through In-Vitro Colchicines Treatment

Istiqomah

Shofi

2018

sceducatia

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Pineapple Plant (Ananas comosus (L.) Merr) is a fruit plant which has a high economic value. Increased variety of pineapple preparations lead to increased demand for pineapple fruit. Polyploidation is one way to increase the varieties using colchicine. This study aims to know the pineapple explants of callus response after being treated by colchicine using the in vitro method. This research used Group Randomized Design (GRD) which variations concentration of Colchicine 0%, 0.01%, 0.05%, and 0.1%. Parameters were observed to response and thickness of callus of pineapple explants. Data were analyzed using the F test and DMRT test in which the significance level of 5% with SPSS 17 program. The results showed that pineapple plant explant able to respond colchicine concentration marked with thicker callus. The best colchicine treatment to induce pineapple explants callus was concentration 0.1% of colchicine.

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Section: Introductionmentioning

confidence: 99%

Response of Pineapple Callus (Ananas comosus Merr.) through In-Vitro Colchicines Treatment

Istiqomah

Shofi

2018

sceducatia

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An Insight into Biotechnological Approaches Used for the Improvement of Secondary Metabolites from the Medicinal Aquatic Plant, Water Hyssop (Bacopa monnieri L.)

Aasım

Sameeullah

Karataş

et al. 2019

Natural Bio-Active Compounds

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Synergism study of Bacopa monnieri and Piriformospora indica and its impact on Biomass and metabolite

Kharde,

Kore,

Singh

et al. 2023

Bull Natl Res Cent

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Background A symbiotic connection between Piriformospora indica and Bacopa monnieri (L.) Wettest, obtained through co-cultivation synergism, was found to improve growth, biomass production, and bacoside content in the plants. Brahmi (B. monnieri L.), a well-known Indian plant prized for its memory-boosting properties, has a lengthy history and a premium price tag. Because of its remarkable ability to colonize a wide variety of plant species, the axenically cultivable mycorrhiza-like endophytic fungus P. indica has gained a lot of interest recently. Methods In the current study, fungal spores from recently revived cultures were added to jam bottles next to rooted Brahmi plants for in vitro co-cultivation. The control plants were left without fungal discs. Pre-rooted micro-propagated Brahmi plants were treated with agar discs containing actively growing hyphae. For a period of 3 months, both trials were conducted with a fully randomized setup. Microscopy of the treated and control plant roots verified co-cultivation. Results Microscopic examination of the roots of co-cultivated plants reveals a high degree of colonization with host plants. These endophytic fungal structures include intracellular chlamydospores, and arbuscules, an intercellular and intracellular hyphae network, and a mycelial network on the root surface. In both in vitro and in vivo co-cultivation studies, the plant extended the host plant’s lifespan in 3 months by displaying continuous regeneration; in contrast, the control plant displayed signs of senescence. With biomass exceeding the control by 1.18 times in vivo and 1.28 times in vitro. In vitro, co-cultivation circumstances also led to an increase in the rate of utilization of nutritional medium. In comparison to the control, the amount of bacoside increases to 100% in vivo after a month of co-cultivation and 33% in vitro after 3 months. Conclusions In the present investigation, in vivo co-cultivation showed a favorable interaction effect on biomass production as well as bacoside content, which can satisfy the raw material demands of Brahmi plants in pharmaceutical industries.

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In Vitro Enhancement of Bacoside in Brahmi (Bacopa monnieri) Using Colchicine

Cited by 5 publications

References 24 publications

Response of Pineapple Callus (Ananas comosus Merr.) through In-Vitro Colchicines Treatment

Response of Pineapple Callus (Ananas comosus Merr.) through In-Vitro Colchicines Treatment

An Insight into Biotechnological Approaches Used for the Improvement of Secondary Metabolites from the Medicinal Aquatic Plant, Water Hyssop (Bacopa monnieri L.)

Synergism study of Bacopa monnieri and Piriformospora indica and its impact on Biomass and metabolite

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