An Insight into Biotechnological Approaches Used for the Improvement of Secondary Metabolites from the Medicinal Aquatic Plant, Water Hyssop (Bacopa monnieri L.)

Aasım, Muhammad; Sameeullah, Muhammad; Karataş, Mehmet; Bakirci, Seyma; Bakhsh, Allah; Akhtar, Mohd Sayeed

doi:10.1007/978-981-13-7438-8_5

Cited by 6 publications

(4 citation statements)

References 105 publications

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“…Young plants, seedlings, calli, and somatic embryos are preferred to be used in tissue culture systems ( Isah et al., 2017 ; Secgin and Okumus, 2022 ). Propagating plants in vitro using the tissue culture techniques involve two consecutive steps: the first one is the formation of in vitro cell/callus/cell suspension and protoplast culture and the latter one is direct or indirect organogenesis or somatic embryogenesis via in vitro regeneration ( Aasim et al., 2019 ).…”

Section: Tissue Culture-based Biotechnological Approaches For Obtaini...mentioning

confidence: 99%

Production of secondary metabolites using tissue culture-based biotechnological applications

et al. 2023

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Plants are the sources of many bioactive secondary metabolites which are present in plant organs including leaves, stems, roots, and flowers. Although they provide advantages to the plants in many cases, they are not necessary for metabolisms related to growth, development, and reproduction. They are specific to plant species and are precursor substances, which can be modified for generations of various compounds in different plant species. Secondary metabolites are used in many industries, including dye, food processing and cosmetic industries, and in agricultural control as well as being used as pharmaceutical raw materials by humans. For this reason, the demand is high; therefore, they are needed to be obtained in large volumes and the large productions can be achieved using biotechnological methods in addition to production, being done with classical methods. For this, plant biotechnology can be put in action through using different methods. The most important of these methods include tissue culture and gene transfer. The genetically modified plants are agriculturally more productive and are commercially more effective and are valuable tools for industrial and medical purposes as well as being the sources of many secondary metabolites of therapeutic importance. With plant tissue culture applications, which are also the first step in obtaining transgenic plants with having desirable characteristics, it is possible to produce specific secondary metabolites in large-scale through using whole plants or using specific tissues of these plants in laboratory conditions. Currently, many studies are going on this subject, and some of them receiving attention are found to be taken place in plant biotechnology and having promising applications. In this work, particularly benefits of secondary metabolites, and their productions through tissue culture-based biotechnological applications are discussed using literature with presence of current studies.

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Section: Tissue Culture-based Biotechnological Approaches For Obtaini...mentioning

confidence: 99%

Production of secondary metabolites using tissue culture-based biotechnological applications

et al. 2023

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“…Aasim et al [21] documented that depending on the dose of the mutagen it is negatively correlated with the damage caused in the DNA, it was identified that the damage is greater in the root cellular nuclei, compared to those present in the leaves.…”

Section: Mechanisms Of Action Of Emsmentioning

confidence: 99%

“…They found that this pharmacologically active compound increased by 21%. Aasim et al [21] identified a decrease in the content of bacoside A, by increasing the exposure time of EMS in cells of Bacopa monnieri L. root explants. In leaf explants, regardless of the exposure time, the concentration of bacoside "A" was stable when treated with EMS.…”

Section: Biochemical Characteristics Of Interestmentioning

confidence: 99%

Ethyl Methanesulfonate as Inductor of Somaclonal Variants in Different Crops

Joya-Dávila¹,

Gutiérrez-Miceli²

2020

Phyton

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Ethyl methanesulfonate is a chemical mutagen, which is currently being used in plant breeding, to increase genetic variability in genes of agronomic interest, of species useful in agriculture. It primarily causes single base point mutations by inducing guanine alkylation, resulting in GC to AT transitions. Its effect is different between clones of a genotype and between genotypes of the same species. This review presents the results obtained in recent research, where its effect on plant tissues, callus, and cells in suspension has been evaluated. Changes in the phenotypic expression of somaclonal variants were reported, involving morphology, production of secondary metabolites, changes in metabolic routes of resistance, tolerance to stress, increased seed yield, among others. In addition, this review compiles the doses and guidelines to consider before using this mutagen, which can serve as a guide for future trials in deciding the response variables, the type of plant explants and the selection of the study model. Mutant lines have allowed plant breeders to have a collection of plants with different characteristics, in places where the cultivar does not have its center of origin. It is important to note that it is still necessary to continue evaluating the heritability of mutations and their behaviour in the environment where they will be established, in order to obtain new varieties of plants that can be cultivated with uniformity in their genetic response.

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“…The most commonly used sterilizing agents are NaOCl (sodium hypochlorite), H2O2 (hydrogen peroxide), Ca(ClO)2 (calcium hypochlorite), HgCl2 (mercury II chloride), silver based chemicals like AgNO3 (silver nitrate) or NS (Nano-silver) and PPM (Mihaljević et al, 2013;Nongalleima et al, 2014). Besides that, several other commercial chemicals, antibiotics and fungicides are also employed for in vitro sterilization process (Aasim et al, 2019;Barpete et al, 2021). Another important factor in sterilization is the exposure time of explants to the specific sterilizing agents.…”

Section: Introductionmentioning

confidence: 99%

Optimization of in vitro sterilization for pistachio (Pistacia vera L.) rootstocks

Korkmaz¹,

YAŞAR²,

SOYDAN³

et al. 2022

Anatolian Journal of Botany

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Pistachio (Pistacia vera L.) is one of the leading edible nut consumed all over the World due to its nutritional values. The plant is cultivated in most of the countries alongwith Turkey which is one of the the leading grower of pistachio. In Turkey, the rootstock material is currently propagated through traditional methods and there is a need of propagating plant material using modern biotechnological techniques like plant tissue culture. The provision of contaminated free explants with minimum or no phenolic compounds in the culture medium is the prerequisite of in vitro regeneration protocol. The plant material used in this study was collected at different physiological stages during different months like April- June and Sep-October. The plant material was cut into 2-3 cm long nodal segments followed by cleaning with different agents like water, soap and fungicide prior to subjected to sterilizing agents. Different sterilizing agents used in this study were HgCl2, Huwa-san (H2O2) and commercial bleach (NaOCl) for both rootstocks (UCB-1 and Buttum) with different exposure time. Sterilized explants were cultured on MS basal medium containing plant growth regulators and subcultured once a week for three weeks. Results revealed that HgCl2 as sterilizing agent was more superior than other sterilizing agents for both rootstocks. Among rootstocks, UCB-1 was more responsive than Buttum and relatively more sterilized plants were attained. On the other hand, plant material collected during June responded better and 90.0% and 50.0% sterilzied plants were attained for UCB-1 and Buttum respectively. The results revealed the significant impact of collection time, sterilizing agent type, concentration and exposure time on sterilization of P. vera rootstocks.

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An Insight into Biotechnological Approaches Used for the Improvement of Secondary Metabolites from the Medicinal Aquatic Plant, Water Hyssop (Bacopa monnieri L.)

Cited by 6 publications

References 105 publications

Production of secondary metabolites using tissue culture-based biotechnological applications

Production of secondary metabolites using tissue culture-based biotechnological applications

Ethyl Methanesulfonate as Inductor of Somaclonal Variants in Different Crops

Optimization of in vitro sterilization for pistachio (Pistacia vera L.) rootstocks

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