Simultaneous determination of active component and vehicle penetration from F-DPPC liposomes into porcine skin layers

Mahrhauser, Denise-Silvia; Reznicek, Gottfried; Gehrig, Sebastian; Geyer, Antonia; Ogris, Manfred; Kieweler, Ruth; Valenta, Claudia

doi:10.1016/j.ejpb.2015.10.008

Cited by 11 publications

(5 citation statements)

References 21 publications

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“…Pretreatment with DLPC and DPPG dispersions enhanced the skin permeation of caffeine (Fig. 5), indicating that these phospholipids had skin penetration-enhancing effects, since they might rearrange and fuse with an ordered structure of intercellular lipids (like ceramides) to reduce the SC barrier function made by disruption of well-packed intercellular lipids and creation of a permeation pathway for drugs (Kato et al, 1987;Mahrhauser et al, 2015;Zellmer et al, 1995). Interestingly, the pretreatment of caffeine-free liposomes provided lower ER than their corresponding phospholipid dispersions (Fig.…”

Section: Discussionmentioning

confidence: 99%

Selection of phospholipids to design liposome preparations with high skin penetration-enhancing effects

Sakdiset

Okada

Todo

et al. 2018

Journal of Drug Delivery Science and Technology

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The objective of the present study is to search for a good selection method of phospholipids to design liposome preparations with high skin penetration-enhancing effects.Five kinds of phosphatidylcholines and phosphatidylglycerols each were selected. First, phospholipid aqueous dispersions and liposomes containing caffeine as a model drug were tested for their skin penetration-enhancing effects using excised hairless rat skin. As results, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 1,2-dipalmitoyl-sn-glycero-3phosphoglycerol, sodium salt (DPPG) dispersions showed high penetration-enhancing ratio (ER), whereas DPPG, 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) and 1,2dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes showed high ER, suggesting that liposomes had different skin penetration-enhancing mechanisms from phospholipid dispersions. Next, two kinds of experiments were done to clarify the possible mechanism of liposomes as follows: the excised skin was pretreated for 1 h with caffeine-free phospholipid dispersions and liposomes, and caffeine solution was added to determine its skin permeation.Separately, caffeine permeation experiments were done using physical mixture of blank liposomes and caffeine solution (caffeine-spiked liposomes) and caffeine-entrapped liposomes (caffeine was entrapped only in liposomes). As results, DPPG was a promising phospholipid candidate to fabricate liposome formulations with high skin penetration-enhancing effects, since DPPG phospholipid and its liposome vesicles had a combination effect to disrupt the SC lipid barrier as well as could carry both free and entrapped caffeine in the formulation through the skin.

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Section: Discussionmentioning

confidence: 99%

Selection of phospholipids to design liposome preparations with high skin penetration-enhancing effects

Sakdiset

Okada

Todo

et al. 2018

Journal of Drug Delivery Science and Technology

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“…They consist of phospholipids, which are amphiphilic molecules that have a hydrophilic head and a non-polar hydrophobic tail. In an aqueous medium, phospholipids self-organize into bilayer membranes and form spherical hollow shapes with a liquid core [93]. This amphiphilic structure facilitates inclusion of hydrophilic molecules into the nucleus and hydrophobic molecules into the lipid membrane.…”

Section: Liposomesmentioning

confidence: 99%

Textile Materials Modified with Stimuli-Responsive Drug Carrier for Skin Topical and Transdermal Delivery

2021

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Textile materials, as a suitable matrix for different active substances facilitating their gradual release, can have an important role in skin topical or transdermal therapy. Characterized by compositional and structural variety, those materials readily meet the requirements for applications in specific therapies. Aromatherapy, antimicrobial substances and painkillers, hormone therapy, psoriasis treatment, atopic dermatitis, melanoma, etc., are some of the areas where textiles can be used as carriers. There are versatile optional methods for loading the biologically active substances onto textile materials. The oldest ones are by exhaustion, spraying, and a pad-dry-cure method. Another widespread method is the microencapsulation. The modification of textile materials with stimuli-responsive polymers is a perspective route to obtaining new textiles of improved multifunctional properties and intelligent response. In recent years, research has focused on new structures such as dendrimers, polymer micelles, liposomes, polymer nanoparticles, and hydrogels. Numerous functional groups and the ability to encapsulate different substances define dendrimer molecules as promising carriers for drug delivery. Hydrogels are also high molecular hydrophilic structures that can be used to modify textile material. They absorb a large amount of water or biological fluids and can support the delivery of medicines. These characteristics correspond to one of the current trends in the development of materials used in transdermal therapy, namely production of intelligent materials, i.e., such that allow controlled concentration and time delivery of the active substance and simultaneous visualization of the process, which can only be achieved with appropriate and purposeful modification of the textile material.

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“…However, in most of formulations reported in the literature, the phospholipids used in topical liposomes are similar or identical to the skin lipids. This poses a technical limitation to the analysis of their permeation, for which either the use of labelled lipids or advanced techniques such as secondary ions mass spectrometry (SIMS) bioimaging is required [ 74 , 75 ]. The simultaneous tracking of the active ingredient and the other components would allow gaining information on the mechanisms of dermal permeation.…”

Section: Assessment Of Percutaneous Permeation and Biological Effectmentioning

confidence: 99%

Design and development of topical liposomal formulations in a regulatory perspective

Schlich

Musazzi

Campani

et al. 2021

Drug Deliv. and Transl. Res.

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Simultaneous determination of active component and vehicle penetration from F-DPPC liposomes into porcine skin layers

Cited by 11 publications

References 21 publications

Selection of phospholipids to design liposome preparations with high skin penetration-enhancing effects

Selection of phospholipids to design liposome preparations with high skin penetration-enhancing effects

Textile Materials Modified with Stimuli-Responsive Drug Carrier for Skin Topical and Transdermal Delivery

Design and development of topical liposomal formulations in a regulatory perspective

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