Self-assembled graphene quantum dots induced by cytochrome c: a novel biosensor for trypsin with remarkable fluorescence enhancement

Abstract

On the basis of cytochrome c-induced self-assembled graphene quantum dots, we demonstrate a novel fluorescent biosensor for trypsin with remarkable fluorescence enhancement, as well as high selectivity and sensitivity.

Carbon Based Dots and Their Luminescent Properties and Analytical Applications

“…b Sensing Principle of the N-Doped C-Dots based probe for Fe 3+ (upper figure) and PL microscopy images of Hela cells first incubated with N-CDs for 6 h and then incubated with FeCl 3 (lower figure) [91]. c Schematic illustration of the fluorescent biosensor for trypsin based-on self-assembled GQDs [243] Cytochrome c (Cyt c) rich in Fe 3+ could also quench the PL of CDs selectively due to the special coordination interaction between Fe 3+ and the phenolic hydroxyl group of CDs [243]. Cyt c could bind to CDs and completely quench the PL of CDs.…”

Graphene quantum dots in analytical science

“…Biosensing of trypsin used a method of Li et al [111], in which GQDs were selfassembled by induction of cytochrome c (Cyt c), an electron-transfer cationic protein forming complexes with anionic GQDs. Coordination of Cyt c to GQDs strongly quenched their FL emission through adsorption of GQDs on Cyt c, which also promoted aggregation of GQDs by electrostatic attraction.…”

Multi-positively charged dendrimeric nanoparticles induced fluorescence quenching of graphene quantum dots for heparin and chondroitin sulfate detection

“…Therefore, many researchers have devoted their efforts to explore sensing systems for metal ions (Dong et al,. 2014;Ju and Chen, 2014;Sun et al, 2013;Zhang and Chen, 2014), glucose Qu et al, 2013), melamine , trypsin (Li et al, 2013) and others (Fan et al, 2012;Liu et al, 2013;Qian et al, 2014).…”