Multi-positively charged dendrimeric nanoparticles induced fluorescence quenching of graphene quantum dots for heparin and chondroitin sulfate detection

Li, Yan; Sun, Hongcheng; Shi, Fanping; Cai, Nan; Lu, Lehui

doi:10.1016/j.bios.2015.06.032

Cited by 43 publications

(10 citation statements)

References 42 publications

(37 reference statements)

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“…It was significant to compare the present approach with other reported methods for heparin determination. ,,,− First, this method could directly detect heparin. As shown in Table S1, it was not needed to introduce a control medium, such as protamine, polybrene, peptide, and GO, which simplified the assay procedure.…”

Section: Resultsmentioning

confidence: 99%

One-Step Synthesis of Water-Dispersible and Biocompatible Silicon Nanoparticles for Selective Heparin Sensing and Cell Imaging

et al. 2016

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A sensitive and selective fluorescence "turn-off" sensor to detect heparin using water-soluble silicon nanoparticles (Si NPs) was developed for the first time. The Si NPs were synthesized by a simple one-step procedure, which did not need high-temperature and complex modification. The as-prepared Si NPs featured strong fluorescence, favorable biocompatibility, and robust photo- and pH stability. Significantly, the Si NPs were induced to assemble or aggregate via hydrogen bonding, which resulted in the fluorescence of Si NPs quenched. Under the optimized conditions, the linear range was obtained from 0.02 to 2.0 μg/mL, with a limit of detection of 18 ng/mL (equal to 0.004 U/mL). It was lower than the proper therapeutic level of heparin during cardiovascular surgery and long-term therapy. This proposed method was relatively free of interference from heparin analogues, which commonly existed in heparin samples and could possibly affect heparin detection. Moreover, it did not need to introduce any control medium. As expected, the method was successfully applied to detect heparin in human serum samples with satisfactory recovery ranging from 98.8 to 102.5%. The Si NPs were superbly suitable for cell imaging owing to the negligible cytotoxicity and excellent biocompatibility.

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Section: Resultsmentioning

confidence: 99%

One-Step Synthesis of Water-Dispersible and Biocompatible Silicon Nanoparticles for Selective Heparin Sensing and Cell Imaging

et al. 2016

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“…Remarkably, in the presence of negatively charged Hep or CS, a significant recovery in the fluorescence was observed. Under optimal reaction conditions, the observed fluorescence recovery was found to have a linear relation with the concentration of target analytes in the range of 0.04−1.6 µg/ml for Hep and 0.1−2.5 µg/ml for CS with an LOD as low as 0.02 and 0.05 µg/ml for Hep and CS, respectively (Li, Sun, et al, 2015).…”

Section: 76mentioning

confidence: 94%

Recent progress on graphene quantum dots‐based fluorescence sensors for food safety and quality assessment applications

Sharma

Ali

Devaraj

et al. 2021

Comp Rev Food Sci Food Safe

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The versatile photophysicalproperties, high surface-to-volume ratio, superior photostability, higher biocompatibility, and availability of active sites make graphene quantum dots (GQDs) an ideal candidate for applications in sensing, bioimaging, photocatalysis, energy storage, and flexible electronics. GQDsbased sensors involve luminescence sensors, electrochemical sensors, optical biosensors, electrochemical biosensors, and photoelectrochemical biosensors.Although plenty of sensing strategies have been developed using GQDs for biosensing and environmental applications, the use of GQDs-based fluorescence techniques remains unexplored or underutilized in the field of food science and technology. To the best of our knowledge, comprehensive review of the GQDsbased fluorescence sensing applications concerning food quality analysis has not yet been done. This review article focuses on the recent progress on the synthesis strategies, electronic properties, and fluorescence mechanisms of GQDs.The various GQDs-based fluorescence detection strategies involving Förster resonance energy transfer-or inner filter effect-driven fluorescence turn-on and turn-off response mechanisms toward trace-level detection of toxic metal ions, toxic adulterants, and banned chemical substances in foodstuffs are summarized. The challenges associated with the pretreatment steps of complex food matrices and prospects and challenges associated with the GQDs-based fluorescent probes are discussed. This review could serve as a precedent for further advancement in interdisciplinary research involving the development of versatile GQDs-based fluorescent probes toward food science and technology applications.

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“…This interior and exterior “dual-FRET” generated full-color emissionincluding whitein an aqueous medium. The displacement of the green-emitting dye upon the addition of an increasing amount of UFH led to the gradual suppression of one single FRET channel, shifting the visible response from white to blue via yellowish-white. − …”

Section: Heparin Monitoringmentioning

confidence: 99%

Lost in (Clinical) Translation: Recent Advances in Heparin Neutralization and Monitoring

Ourri

Vial

2019

ACS Chem. Biol.

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The heparin family, which includes unfractionated heparin, low-molecular heparin and fondaparinux, is a class of drugs clinically used as intravenous blood thinner. To date, issues related to both to the reversal of anticoagulation and the blood level determination of the anticoagulant at the point-of-care remain: while the only U.S. Food and Drug Administration (FDA) approved antidote for heparin displays serious efficacy and safety drawbacks, the current assays for heparin monitoring are indirect measurements subject to their own limitations and variations. Herein, we provide an update on the numerous recent chemical approaches to tackle these issues, from which it is clear that some new antidotes and sensors for heparin certainly have the potential to exceed current clinical standards. This review aims to review a field that requires close collaborations between physicians, biologists and chemists in order to foster advances toward clinical translation. Context and challengesHeparin, which belongs to the family of glycosaminoglycans (GAGs), is a linear sulfated polysaccharide and consists of repeating disaccharide subunits of α-1,4 linked uronic acid and D-glucosamine (Figure 1). As a consequence, heparin displays the highest density of negative charges among biomacromolecules. This polyanion is widely used as an intravenous anticoagulant due to its ability to accelerate the rate at which antithrombin (AT) inhibits serine proteases within the blood coagulation cascade, most notably factor Xa and thrombin. [1][2][3][4] Polymers of various lengths such as unfractionated heparin (UFH, Mw ~ 15 kDa), low-molecular-weight heparins (LMWHs, Mw = 3.6-6.5 kDa), and the synthetic pentasaccharide fondaparinux (Mw = 1.7 kDa) are routinely delivered to patients. While UFH is used during acute thrombotic events or to maintain blood fluidity during procedures requiring extracorporeal circulation (i.e., cardio-pulmonary bypass or hemodialysis), 5,6 LMWHs and fondaparinux are prescribed to treat and/or prevent deep vein thrombosis and pulmonary embolism. 7,8 With an estimated one billion doses of heparin produced every year, heparin's market is rapidly growing, driven by the ageing of populations and the increasing incidence of cardiovascular diseases. 9 KeywordsAnticoagulants: commonly known as blood thinners, they are chemical substances that retard or inhibit the coagulation of the blood.

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Multi-positively charged dendrimeric nanoparticles induced fluorescence quenching of graphene quantum dots for heparin and chondroitin sulfate detection

Cited by 43 publications

References 42 publications

One-Step Synthesis of Water-Dispersible and Biocompatible Silicon Nanoparticles for Selective Heparin Sensing and Cell Imaging

One-Step Synthesis of Water-Dispersible and Biocompatible Silicon Nanoparticles for Selective Heparin Sensing and Cell Imaging

Recent progress on graphene quantum dots‐based fluorescence sensors for food safety and quality assessment applications

Lost in (Clinical) Translation: Recent Advances in Heparin Neutralization and Monitoring

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