Safe and Effective Removal of Urea by Urease-Immobilized, Carboxyl-Functionalized PES Beads with Good Reusability and Storage Stability

Zhang, Jue; Wang, Zhou‐jun; He, Chao; Liu, Xiaoling; Zhao, Weifeng; Sun, Shudong; Zhao, Changsheng

doi:10.1021/acsomega.8b03287

Cited by 28 publications

(10 citation statements)

References 64 publications

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“…The active site of urease comprises of two nickel(II) atoms (Ni +2 ) [ 16 ]. Urease converts urea into NH 3 and carbon dioxide by hydrolysis [ 17a,b ]. It has been reported that ureases are responsible for pathogenicity such as stomach cancer, peptic ulceration, hepatic coma and urinary stones [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis, density functional theory (DFT) studies and urease inhibition activity of chiral benzimidazoles

Aman

Rashid

Ashraf

et al. 2020

Heliyon

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A variety of benzimidazole by the heterocyclization of orthophenylenediamine were synthesized in 69–86% yields. The synthesized compounds 3a-f and 6a-f were characterized and further investigated as jack bean urease inhibitors. Density functional theory (DFT) studies were performed utilizing the basis set B3LYP/6-31G (d, p) to acquire perception into their structural properties. Frontier molecular orbital (FMO) analysis of all compounds 3a–f and 6a-f was computed at the same level of theory to get a notion about their chemical reactivity and stability. The mapping of the molecular electrostatic potential (MEP) over the entire stabilized molecular geometry indicated the reactive centers. They exhibited urease inhibition activity with IC50 between 22 and 99 μM. Compounds containing withdrawing groups on the benzene ring ( 3d, 6d ) were not showing significant urease inhibition. The value obtained for 3a, 3b, 3f had shown their significant urease inhibition for both theoretical and experimental. Notably, the compound having S-configuration ( 3a ) (22.26 ± 6.2 μM) was good as compared to its R enantiomer 3f (31.42 ± 23.3 μM). Despite this, we elaborated the computational studies of the corresponding compounds, to highlight electronic effect which include HOMO, LUMO, Molecular electrostatic potential (MEP) and molecular docking.

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Section: Introductionmentioning

confidence: 99%

Synthesis, density functional theory (DFT) studies and urease inhibition activity of chiral benzimidazoles

Aman

Rashid

Ashraf

et al. 2020

Heliyon

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“…[ 4,20 ] Besides the antifouling performance, high Ca 2+ adsorption on the LZ/SA adsorbent further contributed to the anticoagulant performance, since calcium can be directly used as a coagulation complex factor to promote the platelet‐mediated coagulation process (Figure S14, Supporting Information). [ 31,32 ] As tested by the calcium ion‐selective electrode, the adsorption capacity of Ca 2+ is improved as the initial concentration is increased (Figure 2l), which is owing to the “egg‐box” structure of the cross‐link between Ca 2+ and alginate chains. [ 33 ] The high adsorption of Ca 2+ led to a decreased risk of thrombus formation upon the LZ/SA adsorbent use.…”

Section: Resultsmentioning

confidence: 99%

Protein/Polysaccharide Composite toward Multi‐in‐One Toxin Removal in Blood with Self‐Anticoagulation and Biocompatibility

Chen

Yang

et al. 2023

Adv Healthcare Materials

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Biocompatible adsorbents play an essential role in hemoperfusion. Nevertheless, there are no hemoperfusion adsorbents that can simultaneously remove small and medium toxins, including bilirubin, urea, phosphor, heavy metals, and antibiotics. This bottleneck significantly impedes the miniaturization and portability of hemoperfusion materials and devices. Herein, a biocompatible protein‐polysaccharide complex is reported that exhibits “multi‐in‐one” removal efficacy for liver and kidney metabolism wastes, toxic metal ions, and antibiotics. Through electrostatic interactions and polysaccharide‐mediated coacervation, adsorbents can be prepared by simply mixing lysozyme (LZ) and sodium alginate (SA) together in seconds. The LZ/SA absorbent presented high adsorption capacities for bilirubin, urea, and Hg2+ of up to 468, 331, and 497 mg g−1, respectively, and the excellent anti‐protein adsorption endowed LZ/SA with a record‐high adsorption capacity for bilirubin in the interference of serum albumin to simulate the physiological environment. The LZ/SA adsorbent also has effective adsorption capacity for heavy metals (Pb2+, Cu2+, Cr3+, and Cd2+) and multiple antibiotics (terramycin, tetracycline, enrofloxacin, norfloxacin, roxithromycin, erythromycin, sulfapyrimidine, and sulfamethoxazole). Various adsorption functional groups exposed on the adsorbent surface significantly contribute to the excellent adsorption capacity. This fully bio‐derived protein/alginate‐based hemoperfusion adsorbent has great application prospects in the treatment of blood‐related diseases.

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“…AA and SSNA were introduced to prepare heparin-mimicking polymer-functionalized MGO (PAS-MGO) via in situ crosslinking polymerization. , In brief, 3 g of AA and 2 g of SSNA (monomers), 0.05 g of MBA (crosslinker), and 0.005 g of APS (initiator) were added into 100 mL of MGO suspension (about 0.3 mg/mL) with mechanical stirring for 30 min. Then, the reaction was proceeded at 75 °C for 24 h under nitrogen atmosphere, and ceased by exposing the mixture in air at room temperature, then the prepared PAS-MGO was separated by a magnet and washed several times.…”

Section: Materials and Methodsmentioning

confidence: 99%

Urease-Immobilized Magnetic Graphene Oxide as a Safe and Effective Urea Removal Recyclable Nanocatalyst for Blood Purification

Zhang

Yan

et al. 2020

Ind. Eng. Chem. Res.

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Chronic kidney disease has attracted great attention as a public health disease and is mainly caused by the retention of uremic toxins in the body. Urea is a kind of representative toxin with high solubility and cannot be removed easily; in fact, no suitable absorbers have been found to remove urea by physical adsorption. Herein, we prepare urease-immobilized magnetic graphene oxide as a recyclable nanocatalyst for effective urea removal in blood purification, which shows great removal ability toward urea (2194 ± 110 mg/g). The clearance ratio is over 80% even for a high concentration urea solution of 80 mg/mL, and it is 77% for collected dialysate from a clinic. The nanocatalyst exhibits good reusability and storage stability. Furthermore, a series of blood-related tests indicate that the prepared nanocatalyst has good blood compatibility. Thus, the urease-immobilized recyclable nanocatalyst has great potential for effective and safe urea removal in blood purification.

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Safe and Effective Removal of Urea by Urease-Immobilized, Carboxyl-Functionalized PES Beads with Good Reusability and Storage Stability

Cited by 28 publications

References 64 publications

Synthesis, density functional theory (DFT) studies and urease inhibition activity of chiral benzimidazoles

Synthesis, density functional theory (DFT) studies and urease inhibition activity of chiral benzimidazoles

Protein/Polysaccharide Composite toward Multi‐in‐One Toxin Removal in Blood with Self‐Anticoagulation and Biocompatibility

Urease-Immobilized Magnetic Graphene Oxide as a Safe and Effective Urea Removal Recyclable Nanocatalyst for Blood Purification

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