Rumen fermentation patterns and nutrient digestion in lambs fed cottonseed meal supplemental diets

Nagalakshmi, D.; Sastry, V. R. B.; Pawde, A.

doi:10.1016/s0377-8401(02)00140-2

Cited by 26 publications

(14 citation statements)

References 21 publications

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“…Furthermore, toxic effects of gossypol are reported to be much greater in non-ruminants including fish (Willard et al, 1995). To remove gossypol from cottonseed, solvent extraction (Canella and Sodini, 1977;Cherry and Gray, 1981), chemical treatment with ferrous sulfate (Lim and Lee, 2009;Barraza et al, 1991) or calcium hydroxide (Nagalakshmi et al, 2002;Nagalakshmi et al, 2003), and microbial fermentation (Zhang et al, 2006;Zhang et al, 2007) have been tested for their efficacy. Microbial fermentation can offer a practical solution to gossypol detoxification because fermented cottonseed meal usually contains certain types of exoenzymes such as cellulolytic enzyme, amylase, protease, and lipolytic enzymes as well as some vitamins (Brock et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Effects of Fermented Cottonseed and Soybean Meal with Phytase Supplementation on Gossypol Degradation, Phosphorus Availability, and Growth Performance of Olive Flounder (Paralichthys olivaceus)

임세진¹,

김성삼²,

Pham³

et al. 2010

Fisheries and aquatic sciences

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To reduce anti-nutritional factors in plant protein sources for fish meal replacement in fish feeds, cottonseed and soybean meal (CS) were fermented with Aspergillus oryzae. A feeding trial was conducted to verify the effects of fermented CS (FCS) with phytase supplementation on gossypol detoxification, phosphorus digestibility, antioxidant activity, and growth performance of juvenile olive flounder over 10 weeks. Four diets were formulated to replace 0, 30, or 40% fish meal protein with CS or FCS (designated as CS0, CS30, FCS30P, and FCS40P). Phytase (1,000 FTU/kg) was added to FCS30P and FCS40P. The microbial fermentation significantly increased dietary total polyphenols and consequently led to higher DPPH radical-scavenging activities in fish feed and fish tissue. Dietary and liver gossypol concentrations were dramatically decreased by the fermentation process. Phosphorus digestibility was significantly increased in fish fed the FCS40P diet. However, growth performance decreased in fish fed FCS diets. This study demonstrates that the fermentation process and phytase supplementation can improve the phosphorus availability of plant protein sources in fish. The fermentation of CS by A. oryzae could increase antioxidant activities in feed and fish and effectively degrade toxic gossypol in cottonseed meal.

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Section: Introductionmentioning

confidence: 99%

Effects of Fermented Cottonseed and Soybean Meal with Phytase Supplementation on Gossypol Degradation, Phosphorus Availability, and Growth Performance of Olive Flounder (Paralichthys olivaceus)

임세진¹,

김성삼²,

Pham³

et al. 2010

Fisheries and aquatic sciences

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“…Consequently glandless cottonseed accounts for less than 0.5% of the total crop in the world. A number of methods have been developed for removing gossypol from cottonseed including solvent extraction of free gossypol (Damaty and Hudson, 1975;Canella and Sodini, 1977;Cherry and Gray, 1981;Rahma and Narasingo Rao, 1984), ferrous sulfate treatment (Tabatabai et al, 2002;Barraza et al, 1991), calcium hydroxide treatment (Nagalakshmi et al, 2002;2003), microbial fermentation (Wu and Chen, 1989;Shi et al, 1998) and so on. These methods play an important role in detoxification of CSM, but the reduction of gossypol using solvent system suffers from the difficulty of totally removing residual solvents which have potentially harmful effects, as adversely affect the flavor.…”

Section: Introductionmentioning

confidence: 99%

Effect of selected fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal

Zhang

Sun

et al. 2006

J. Zhejiang Univ. - Sci. B

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Abstract:The objective of this work was to investigate the effect of six individual strains of fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal (CSM). Six groups of disinfected CSM substrate were incubated for 48 h after inoculation with either of the fungi C. capsuligena ZD-1, C. tropicalis ZD-3, S. cerevisae ZD-5, A. terricola ZD-6, A. oryzae ZD-7, or A. niger ZD-8. One not inoculated group (substrate) was used as a control. Levels of initial and final free gossypol (FG), crude protein (CP), amino acids (AA) and in vitro digestibility were assayed. The experiment was done in triplicate.The experimental results indicated that microbial fermentation could greatly decrease (P<0.05) FG levels in CSM. The detoxification efficiency differed between the species of microorganisms applied. From the perspective of reducing CSM potential toxicity, C. tropicalis ZD-3 was most successful followed by S. cerevisae ZD-5 and A. niger ZD-8. They could reduce FG levels of CSM to 29.8, 63.07 and 81.50 mg/kg based on DM (dry matter), respectively, and their detoxification rates were 94.57%, 88.51% and 85.16%, respectively. If crude protein, amino acids content and their in vitro digestibility were also taken into account, A. niger ZD-8 may be the best choice. The CP content of CSM substrate fermented by C. tropicalis ZD-3 and A. niger ZD-8 were improved by 10.76% and 22.24%; the TAA (total amino acids) contents were increased by 7.06% and 11.46%, and the EAA (essential amino acids) were raised by 7.77% and 12.64%, respectively. Especially, the levels of methionine, lysine and threonine were improved greatly (P<0.05). The in vitro CP digestibility of CSM fermented by C. tropicalis ZD-3 and A. niger ZD-8 was improved by 13.42% and 18.22%, the TAA were increased by 17.75% and 22.88%, and the EAA by 16.61% and 21.01%, respectively. In addition, the in vitro digestibility of methionine, lysine and threonine was also improved greatly (P<0.05).

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“…As for cottonseed meal (CSM), an important protein feedstuff, three methods have been commonly used in animal feed processing for many years to reduce gossypol's toxicity, including i) mechanical processing [9], ii) chemical treatment [10,11], and iii) microbial fermentation [12]. Among these methods, microbial fermentation was considered the most promising method for gossypol detoxification as well as a nutritional improvement compared with the other methods with subsiding effects, especially for CSM [13,14].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of a Rumen Lactobacillus Bacteria and Its Degradation Potential of Gossypol in Cottonseed Meal during Solid-State Fermentation

Wang

et al. 2021

Microorganisms

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Cottonseed meal (CSM) is an important protein feed source for dairy cows. Its inclusion in ruminant diets is limited due to the presence of the highly toxic gossypol though rumen microorganisms are believed to be capable of gossypol degrading and transforming. The objective of the present study was to isolate the gossypol-degrading bacteria from the rumen contents and to assess its potential for gossypol degradation in vitro. A strain named Lactobacillus agilis WWK129 was anaerobically isolated from dairy cows after mixed rumen microorganisms were grown on a substrate with gossypol as the sole carbon source. Furthermore, the strain was applied at 5% inoculum concentration in vitro to continuously ferment CSM at 39 °C for five days, and it presented gossypol degradability as high as 83%. Meanwhile, the CSM contents of crude protein, essential amino acids increased significantly along with the increase of lactic acid yield (p < 0.01). Compared with the original CSM, the fermented CSM contents of neutral detergent fiber and acid detergent fiber was remarkably decreased after the anaerobic fermentation (p < 0.01). In brief, the Lactobacillus strain isolated from the rumen is not only of great importance for gossypol biodegradation of CSM, but it could also be used to further explore the role of rumen microorganisms in gossypol degradation by the ruminants.

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Rumen fermentation patterns and nutrient digestion in lambs fed cottonseed meal supplemental diets

Cited by 26 publications

References 21 publications

Effects of Fermented Cottonseed and Soybean Meal with Phytase Supplementation on Gossypol Degradation, Phosphorus Availability, and Growth Performance of Olive Flounder (Paralichthys olivaceus)

Effects of Fermented Cottonseed and Soybean Meal with Phytase Supplementation on Gossypol Degradation, Phosphorus Availability, and Growth Performance of Olive Flounder (Paralichthys olivaceus)

Effect of selected fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal

Isolation and Identification of a Rumen Lactobacillus Bacteria and Its Degradation Potential of Gossypol in Cottonseed Meal during Solid-State Fermentation

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