Effects of Fermented Cottonseed and Soybean Meal with Phytase Supplementation on Gossypol Degradation, Phosphorus Availability, and Growth Performance of Olive Flounder (Paralichthys olivaceus)

Abstract

To reduce anti-nutritional factors in plant protein sources for fish meal replacement in fish feeds, cottonseed and soybean meal (CS) were fermented with Aspergillus oryzae. A feeding trial was conducted to verify the effects of fermented CS (FCS) with phytase supplementation on gossypol detoxification, phosphorus digestibility, antioxidant activity, and growth performance of juvenile olive flounder over 10 weeks. Four diets were formulated to replace 0, 30, or 40% fish meal protein with CS or FCS (designated as CS0, CS30, FCS30P, and FCS40P). Phytase (1,000 FTU/kg) was added to FCS30P and FCS40P. The microbial fermentation significantly increased dietary total polyphenols and consequently led to higher DPPH radical-scavenging activities in fish feed and fish tissue. Dietary and liver gossypol concentrations were dramatically decreased by the fermentation process. Phosphorus digestibility was significantly increased in fish fed the FCS40P diet. However, growth performance decreased in fish fed FCS diets. This study demonstrates that the fermentation process and phytase supplementation can improve the phosphorus availability of plant protein sources in fish. The fermentation of CS by A. oryzae could increase antioxidant activities in feed and fish and effectively degrade toxic gossypol in cottonseed meal.

The effects of different fishmeal level diets with or without phytase supplementation on growth performance, body composition, digestibility, immunological and biochemical parameters of juvenile hybrid sturgeon ( Acipenser baeri Brandt ♀ × A. schrenckii Brandt ♂)

“…The reason for this result may be FM100p group released more phytic acid when phytase was added, whereas phytic acid interfered with the formation of ADPiron-oxygen complexes that initiate lipid peroxidation, which can inhibit lipid peroxidation and decrease the MDA content (Muraoka & Miura, 2004). This was supported by the study that the fermented cottonseed and soybean meal with phytase supplementation could increase antioxidant activities of olive flounder (Paralichthys olivaceus; Lim et al, 2010). Similar results were shown in other fish species, such as yellow catfish (Pelteobagrus fulvidraco; Wang et al, 2017) and grass carp (Ctenopharyngodon idellus; Ma et al, 2011).…”

Effects of partial substitution of dietary fish meal by fermented soybean meal on growth performance, amino acid and biochemical parameters of juvenile black sea breamAcanthopagrus schlegeli

“…The FSM used in this study was supplied by CJ Che-ilJedang (Seoul, Korea). The FSM was fermented using Bacillus subtilis using a process modified to method as mentioned in the Lim et al (2010). Briefly, soybean meal was steam cooked in an autoclave at 100°C for 20 min (pH 5-6).…”

“…Fermentation is a process which allows microorganisms, such as Bacillus subtilis (Cohn), to degrade protein macromolecules to a large extent water-soluble low molecular weight compounds (Kiers, Laeken, Rombouts & Nout 2000). It was found that fermentation of soybean meal induced removal or inactivation of ANFs (Reddy & Pierson 1994;Lim, Kim, Pham, Song, Cha, Kim, Kim & Lee 2010), improvement of the nutritional quality (Canella, Bernardi & Marghinott 1984), improvement of digestibility (Kiers et al 2000) and shelf life of the processed food (Skrede & Nes 1988). Fermented fish silage and fermented soybean meal (FSM) were reported as suitable protein sources in the diets of catfish, Clarias gariepinus (Burchell) and Nile tilapia Oreochromis niloticus L. (Fagbenro, Jauncey & Haylor 1994).…”

Fitat dan fitase : dampak pada hewan ternak

“…Selain bijian, fermentasi juga dilakukan pada limbah bijian seperti bungkil kedelai. Lim et al (2010) menyatakan bahwa fermentasi bungkil kedelai akan menghilangkan atau menginaktivasi faktor antinutrisi. Fermentasi tersebut juga akan meningkatkan nilai nutrisi dan peningkatan kecernaan (Kiers et al, 2000).…”