Role of apoptotic index, mitotic index and MIB-1 antibody expression as biomarkers in preneoplastic and neoplastic lesions of uterine cervix

Chauhan, Rashmi Singh; Verma, Nidhi; Sharma, Shipra; Bhargava, Ruchika; Singh, Preeti

doi:10.18203/2320-6012.ijrms20161767

Cited by 7 publications

(8 citation statements)

References 19 publications

(33 reference statements)

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“…In the present study Ki-67 index increased with increasing grade of dysplasia. 6 In the present study, there was an increase in mean labelling index with increasing grade of dysplasia, from CIN-1 (6.25±2.04) to CIN-2 (19.75±4.13) to CIN-3 (34.81±3.27) and the p value was found to be statistically significant (p<0.0001) which is similar to Gogoi et al study, Chauhan et al and Gupta et al 5,6,8 In the present study the mean labelling index and standard deviation in keratinizing SCC (56.05±4.05), non-keratinizing SCC is (46.133±2.34). Correlation between keratinizing SCC and non-keratinizing SCC was found to be statistically significant, (p<0.05) which correlated with Gupta et al and Chauhan et al 6,8 In the study by Gogoi et al did not show any statistically significant difference.…”

Section: Discussionsupporting

confidence: 88%

“…6 In the present study, there was an increase in mean labelling index with increasing grade of dysplasia, from CIN-1 (6.25±2.04) to CIN-2 (19.75±4.13) to CIN-3 (34.81±3.27) and the p value was found to be statistically significant (p<0.0001) which is similar to Gogoi et al study, Chauhan et al and Gupta et al 5,6,8 In the present study the mean labelling index and standard deviation in keratinizing SCC (56.05±4.05), non-keratinizing SCC is (46.133±2.34). Correlation between keratinizing SCC and non-keratinizing SCC was found to be statistically significant, (p<0.05) which correlated with Gupta et al and Chauhan et al 6,8 In the study by Gogoi et al did not show any statistically significant difference. 5 In the present study there is a significant difference of mean Ki-67 labelling index between premalignant and malignant lesions of the cervix which is similar to studies done by Gogoi et al, Gupta et al and Chauhan R et al 5,6,8 Apoptosis is genetically regulated and it permits the elimination of damaged cells.…”

Section: Discussionsupporting

confidence: 88%

“…Correlation between keratinizing SCC and non-keratinizing SCC was found to be statistically significant, (p<0.05) which correlated with Gupta et al and Chauhan et al 6,8 In the study by Gogoi et al did not show any statistically significant difference. 5 In the present study there is a significant difference of mean Ki-67 labelling index between premalignant and malignant lesions of the cervix which is similar to studies done by Gogoi et al, Gupta et al and Chauhan R et al 5,6,8 Apoptosis is genetically regulated and it permits the elimination of damaged cells. 4 Relationship of Apoptosis with tumour growth and progression has been studied by several authors and apoptotic index is included among parameters used to measure tumour growth in various malignancies including cervical carcinoma.…”

Section: Discussionsupporting

confidence: 65%

“…The biologic behaviour of the tumour depends not only on its proliferative activity index but also on the number of cells dying by apoptosis. 8 Almost all invasive cervical cancers are preceded by dysplasia and infection with high risk HPV. 9 Early identification of CIN-1 is the most suitable method to detect the risk of cervical cancer.…”

Section: Discussionmentioning

confidence: 99%

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Clinicopathological study of premalignant and malignant lesions of cervix along with apoptotic index and Ki-67 expression

Atla¹,

Uma²,

Samantra³

et al. 2020

Int J Res Med Sci

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Background: Cervical cancer is known to have a good response to radiotherapy. The response and prognosis are dependent on the level of apoptosis. Pap smear and histopathology are cost-effective methods in diagnosing premalignant and malignant lesions of cervix but not accurate in classifying and estimating the progression of the disease, especially in premalignant lesions. Therefore this study was undertaken to know the role of Ki-67 expression and apoptotic index in classifying accurately the premalignant lesions for better management.Methods: The study included 540 cases diagnosed histologically as cervical intraepithelial neoplasia or carcinoma. The apoptotic index is calculated for all the 540 cases using light microscopy on Haematoxylin and Eosin stained sections. Ki-67 immunohistochemical staining was done for 100 cervical biopsies. Ki-67 expression was graded and the Ki-67 labelling index was calculated. Statistical evaluation was done using the unpaired t-test.Results: The Apoptotic index increased with increasing grade of dysplasia. There is a significant difference in the mean apoptotic index between premalignant and malignant lesions of the cervix. The ki-67 index increased with increasing grade of dysplasia. There is a significant difference in the mean Ki-67 index between premalignant and malignant lesions of the cervix.Conclusions: Apoptotic index and proliferative indices have been found useful in distinguishing between premalignant and malignant lesions of the cervix and gives an idea about the proliferative activity of the tumour for better management of the patient and to determine prognosis.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Clinicopathological study of premalignant and malignant lesions of cervix along with apoptotic index and Ki-67 expression

Atla¹,

Uma²,

Samantra³

et al. 2020

Int J Res Med Sci

View full text Add to dashboard Cite

show abstract

“…Tumor grading was done according to WHO/ISUP grading criteria [9]. The mitosis and apoptosis were counted semi quantitatively in ten randomly selected high power fields [10]. Depth of invasion and staging of the tumor were defined according to TNM American Joint Committee on Cancer-Union International Center Cancer staging system (AJCC-UICC) which classifies the tumor histologically as NMIBC (stage pTa and pT1) or MIBC (stage pT2, pT3 and pT4) [9].…”

Section: Histopathological Assessmentmentioning

confidence: 99%

ROC-1, P21 and CAIX as markers of tumor aggressiveness in bladder carcinoma in Egyptian patients

et al. 2020

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Background: Bladder cancer (BC) is one of the most common malignancies in Egypt, representing about 8.7% of cancers in both sexes with more predominance in males, making identification of valuable predictive and prognostic markers, mandatory. Cullin-RING ligases (CRL) play an important role in the ubiquitination of cell cycle-related proteins or other proteins (e.g., DNA replication protein, signal transduction protein). Regulator of Cullins-1 (ROC-1) is a key subunit of CRL. P21 belongs to the family of cyclin dependent kinase inhibitors (CKIs) which regulates cell cycle by inactivating Cyclin-Dependent Kinases key regulators of the cell cycle. CAIX a highly active member of the family of carbonic anhydrases has gained much interest as a hypoxic marker. Hypoxia is a consequence of the rapid growth of many tumors, including bladder cancer, and is an important regulator of gene expression and resistance to chemotherapy and radiotherapy. Therefore the purpose of this study is to evaluate the role of ROC-1, CAIX and P21 and its relationship with the clinico-pathological features of bladder cancer in Egyptian patients. Methods: Using the standard immunohistochemical technique, ROC-1, CAIX and P21 expression in 80 primary bladder carcinomas and 15 normal bladder specimens as control group were assessed. The bladder carcinoma cases included 50 cases with muscle invasive bladder cancer and 30 cases with non-muscle invasive bladder cancer. Results: Over expression of ROC-1, CAIX and P21 in BC were significantly associated with muscularis propria invasion and high grade BC. ROC-1, CAIX and P21, showed significant inverse relationship in primary BC cases. CAIX expression was significantly higher in BC compared with controls. Regarding the survival analysis, expression of ROC-1, CAIX and P21 didn't affect the survival of BC patients. Conclusions: High expression of ROC-1, CAIX and P21 could be promising potential biomarkers for identifying patients with poor prognostic factors in bladder cancer serving as potential targets for cancer therapy.

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Stratification of urothelial bladder carcinoma depending on immunohistochemical expression of GATA3 and CK5/6

El-Dien

Abdou

Elghrabawy

et al. 2021

Journal of Immunoassay and Immunochemistry

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Role of apoptotic index, mitotic index and MIB-1 antibody expression as biomarkers in preneoplastic and neoplastic lesions of uterine cervix

Cited by 7 publications

References 19 publications

Clinicopathological study of premalignant and malignant lesions of cervix along with apoptotic index and Ki-67 expression

Clinicopathological study of premalignant and malignant lesions of cervix along with apoptotic index and Ki-67 expression

ROC-1, P21 and CAIX as markers of tumor aggressiveness in bladder carcinoma in Egyptian patients

Stratification of urothelial bladder carcinoma depending on immunohistochemical expression of GATA3 and CK5/6

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