Restorative and pain-relieving effects of fibroin in preclinical models of tendinopathy

Micheli, Laura; Parisio, Carmen; Lucarini, Elena; Carrino, Donatello; Ciampi, Clara; Toti, Alessandra; Ferrara, Valentina; Pacini, Alessandra; Ghelardini, Carla; Mannelli, Lorenzo

doi:10.1016/j.biopha.2022.112693

Cited by 6 publications

(6 citation statements)

References 96 publications

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“…Carrageenan was solubilized in a physiological solution. The control animals were treated with saline solution [ 27 , 28 ]. Twenty μL of HA-LP-TQ1 (1 mg/mL) or HA-LP-TQ2 (2 mg/mL), HA-LP, LP-TQ1 (2 mg/mL) formulations and a saturated aqueous solution of TQ (0.55 mg/mL) were peri-tendon injected on days 1, 3, 5, 7 and 10.…”

Section: Methodsmentioning

confidence: 99%

“…The Rota rod apparatus (Ugo Basile, Varese, Italy) consisted of a base platform and a rotating rod with a diameter of 6 cm and a non-slippery surface and was used as a measure of motor coordination. Full details were reported in a previous paper by Micheli and colleagues [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, tendon samples were fixed in buffered 4% formalin, dehydrated and embedded in paraffin (Bio-Optica, Milan, Italy). Ten µm thickness longitudinal sections were processed for Hematoxylin-Eosin (Bio-Optica, Milan, Italy), as previously described [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

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Antinociceptive Action of Thymoquinone-Loaded Liposomes in an In Vivo Model of Tendinopathy

Micheli,

Di Cesare Mannelli,

Mosti

et al. 2023

Pharmaceutics

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Tendinopathies represent about 45% of musculoskeletal lesions and they are a big burden in clinics characterized by activity-related pain, focal tendon tenderness and intra-tendinous imaging changes. Many approaches have been proposed for tendinopathies’ management (e.g., nonsteroidal anti-inflammatory drugs, corticosteroids, eccentric exercises, laser therapy), unfortunately with very little support of efficacy or serious side effects, thus making the identification of new treatments fundamental. The aim of the study was to test the protective and pain reliever effect of thymoquinone (TQ)-loaded formulations in a rat model of tendinopathy induced by carrageenan intra-tendon injection (20 µL of carrageenan 0.8% on day 1). Conventional (LP-TQ) and hyaluronic acid (HA)-coated TQ liposomes (HA-LP-TQ) were characterized and subjected to in vitro release and stability studies at 4 °C. Then, TQ and liposomes were peri-tendon injected (20 µL) on days 1, 3, 5, 7 and 10 to evaluate their antinociceptive profile using mechanical noxious and non-noxious stimuli (paw pressure and von Frey tests), spontaneous pain (incapacitance test) and motor alterations (Rota rod test). Liposomes containing 2 mg/mL of TQ and covered with HA (HA-LP-TQ2) reduced the development of spontaneous nociception and hypersensitivity for a long-lasting effect more than the other formulations. The anti-hypersensitivity effect matched with the histopathological evaluation. In conclusion, the use of TQ encapsulated in HA-LP liposomes is suggested as a new treatment for tendinopathies.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Antinociceptive Action of Thymoquinone-Loaded Liposomes in an In Vivo Model of Tendinopathy

Micheli,

Di Cesare Mannelli,

Mosti

et al. 2023

Pharmaceutics

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“…The scaffold made of natural biodegradable polymer plays a temporary role in supporting the damaged tendon in the human body. During the synthesis and recovery of natural tissue, it can slowly degrade and produce non-toxic metabolites, which are absorbed by surrounding tissues [ 33 ]. Here, we briefly discuss the role of some natural biodegradable polymers in tendon repair.…”

Section: Application Of Biodegradable Polymers In Tendon Repairmentioning

confidence: 99%

Biodegradable Polymer Electrospinning for Tendon Repairment

et al. 2023

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With the degradation after aging and the destruction of high-intensity exercise, the frequency of tendon injury is also increasing, which will lead to serious pain and disability. Due to the structural specificity of the tendon tissue, the traditional treatment of tendon injury repair has certain limitations. Biodegradable polymer electrospinning technology with good biocompatibility and degradability can effectively repair tendons, and its mechanical properties can be achieved by adjusting the fiber diameter and fiber spacing. Here, this review first briefly introduces the structure and function of the tendon and the repair process after injury. Then, different kinds of biodegradable natural polymers for tendon repair are summarized. Then, the advantages and disadvantages of three-dimensional (3D) electrospun products in tendon repair and regeneration are summarized, as well as the optimization of electrospun fiber scaffolds with different bioactive materials and the latest application in tendon regeneration engineering. Bioactive molecules can optimize the structure of these products and improve their repair performance. Importantly, we discuss the application of the 3D electrospinning scaffold’s superior structure in different stages of tendon repair. Meanwhile, the combination of other advanced technologies has greater potential in tendon repair. Finally, the relevant patents of biodegradable electrospun scaffolds for repairing damaged tendons, as well as their clinical applications, problems in current development, and future directions are summarized. In general, the use of biodegradable electrospun fibers for tendon repair is a promising and exciting research field, but further research is needed to fully understand its potential and optimize its application in tissue engineering.

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“…Silk has good strength, toughness and an excellent degradation rate in vivo [ 45 ]. In vivo, the tensile strength of silk fiber can often maintain a good level for one year and degrade entirely within 2 years, allowing the mechanical load to be gradually transferred from the scaffold to the new ligament [ 46 ]. Structural scaffolds are an essential part of tissue regeneration.…”

Section: Products Of Tendon and Ligament Reconstruction And Repairmentioning

confidence: 99%

Functional biomaterials for tendon/ligament repair and regeneration

Tang

Wang

Xiang

et al. 2022

Regenerative Biomaterials

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With an increase in life expectancy and the popularity of high-intensity exercise, the frequency of tendon and ligament injuries has also increased. Owing to the specificity of its tissue, the rapid restoration of injured tendons and ligaments is challenging for treatment. This review summarises the latest progress in cells, biomaterials, active molecules, and construction technology in treating tendon/ligament injuries. The characteristics of supports made of different materials and the development and application of different manufacturing methods are discussed. The development of natural polymers, synthetic polymers, and composite materials has boosted the use of scaffolds. In addition, the development of electrospinning and hydrogel technology has diversified the production and treatment of materials. First, this paper briefly introduces the structure, function, and biological characteristics of tendons/ligaments. Then, it summarises the advantages and disadvantages of different materials, such as natural polymer scaffolds, synthetic polymer scaffolds, composite scaffolds, and ECM-derived biological scaffolds, in the application of tendon/ligament regeneration. We then discuss the latest applications of electrospun fiber scaffolds and hydrogels in regeneration engineering. Finally, we discuss the current problems and future directions in the development of biomaterials for restoring damaged tendons and ligaments.

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Restorative and pain-relieving effects of fibroin in preclinical models of tendinopathy

Cited by 6 publications

References 96 publications

Antinociceptive Action of Thymoquinone-Loaded Liposomes in an In Vivo Model of Tendinopathy

Antinociceptive Action of Thymoquinone-Loaded Liposomes in an In Vivo Model of Tendinopathy

Biodegradable Polymer Electrospinning for Tendon Repairment

Functional biomaterials for tendon/ligament repair and regeneration

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