Background:The clinical characteristics of novel coronavirus disease (COVID-2019) patients outside the epicenter of Hubei Province are less understood. Methods:We analyzed the epidemiological and clinical features of all COVID-2019 cases in the only referral hospital in Shenzhen Results: Among the 298 confirmed cases, 233 (81.5%) had been to Hubei, while 42 (14%) did not have a clear travel history. Only 218 (73.15%) cases had a fever as the initial symptom. Compared with the nonsevere cases, severe cases were associated with older age, those with underlying diseases, and higher levels of C-reactive protein, interleukin-6, and erythrocyte sedimentation rate. Slower clearance of the virus was associated with a higher risk of progression to critical condition. As of March 6, 2020, 268 (89.9%) patients were discharged and the overall case fatality ratio was 1.0%. Conclusions:In a designated hospital outside Hubei Province, COVID-2019 patients could be effectively managed by properly using the existing hospital system.Mortality may be lowered when cases are relatively mild, and there are sufficient medical resources to care and treat the disease.

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Highly effective and highly efficient decontamination of mercury from aqueous media remains a serious task for public health and ecosystem protection. Here we report that this task can be addressed by creating a mercury 'nano-trap' as illustrated by functionalizing a high surface area and robust porous organic polymer with a high density of strong mercury chelating groups. The resultant porous organic polymer-based mercury 'nano-trap' exhibits a recordhigh saturation mercury uptake capacity of over 1,000 mg g À 1 , and can effectively reduce the mercury(II) concentration from 10 p.p.m. to the extremely low level of smaller than 0.4 p.p.b. well below the acceptable limits in drinking water standards (2 p.p.b.), and can also efficiently remove 499.9% mercury(II) within a few minutes. Our work therefore presents a new benchmark for mercury adsorbent materials and provides a new perspective for removing mercury(II) and also other heavy metal ions from contaminated water for environmental remediation.

The cleavages at the junctions of the flavivirus nonstructural (NS) proteins NS2A/NS2B, NS2B/NS3, NS3/NS4A, and NS4B/NS5 share an amino acid sequence motif and are presumably catalyzed by a virus-encoded protease. We constructed recombinant vaccinia viruses expressing various portions of the NS region of the dengue virus type 4 polyprotein. By analyzing immune precipitates of 35S-labeled lysates of recombinant virus-infected cells, we could monitor the NS2A/NS2B, NS2B/NS3, and NS3/NS4A cleavages. A polyprotein composed of NS2A, NS2B, and the N-terminal 184 amino acids of NS3 was cleaved at the NS2A/NS2B and NS2B/NS3 junctions, whereas a similar polyprotein containing only the first 77 amino acids of NS3 was not cleaved. This finding is consistent with the proposal that the N-terminal 180 amino acids of NS3 constitute a protease domain. Polyproteins containing NS2A and NS3 with large in-frame deletions of NS2B were not cleaved at the NS2A/NS2B or NS2B/NS3 junctions. Coinfection with a recombinant expressing NS2B complemented these NS2B deletions for NS2B/NS3 cleavage and probably also for NS2A/NS2B cleavage. Thus, NS2B is also required for the NS2A/NS2B and NS2B/NS3 cleavages and can act in trans. Other experiments showed that NS2B was needed, apparently in cis, for NS3/NS4A cleavage and for a series of internal cleavages in NS3. Indirect evidence that NS3 can also act in trans was obtained. Models are discussed for a two-component protease activity requiring both NS2B and NS3.

Two distinct types of magnetoresistance oscillations are observed in two electronic Fabry-Pérot interferometers of different sizes in the integer quantum Hall regime. Measuring these oscillations as a function of magnetic field and gate voltages, we observe three signatures that distinguish the two types. The oscillations observed in a 2.0 µm 2 device are understood to arise from the Coulomb blockade mechanism, and those observed in an 18 µm 2 device from the Aharonov-Bohm mechanism. This work clarifies, provides ways to distinguish, and demonstrates control over, these distinct physical origins of resistance oscillations seen in electronic Fabry-Pérot interferometers.Mesoscopic electronics can exhibit wave-like interference effects [1, 2, 3, 4], particle-like charging effects [5], or a complex mix of both [6]. Experiments over the past two decades have investigated the competition between wave and particle properties [7], as well as regimes where they coexist [6,8,9,10]. The electronic Fabry-Pérot interferometer (FPI)-a planar two-contact quantum dot operating in the quantum Hall regime-is a system where both interference and Coulomb interactions can play important roles. This device has attracted particular interest recently due to predicted signatures of fractional [11] and non-Abelian [12,13,14] statistics. The interpretation of experiments, however, is subtle, and must account for the interplay or charging and interference effects in these coherent confined structures.Early measurements by van Wees et al.[15] demonstrated resistance oscillations as a function of magnetic field in an electronic FPI, with an interpretation given in terms of Aharonov-Bohm (AB) interference of edge states. More recently, experimental [16,17,18,19] and theoretical [20,21,22] investigations indicate that Coulomb interaction plays a critical role in these previously observed conductance oscillations-as a function of both magnetic field and electrostatic gate voltagesuggesting an interpretation in terms of field-or gatecontrolled Coulomb blockade (CB). Other recent experiments studying fractional charge and statistics in FPI's [23,24] interpret resistance oscillations as arising from AB interference while taking the gate-voltage period as indicating a change of a quantized charge.In this Letter, we report oscillations of resistance as a function of perpendicular magnetic field, B, and gate voltage in FPI's of different sizes. Oscillations in the smaller (2.0 µm 2 ) device are consistent with the interacting (CB) interpretation, while those in the larger (18 µm 2 ) device are consistent with noninteracting AB interference. Specifically, three signatures that distinguish the two types of oscillations are presented: The magnetic field period is roughly proportional to B for CB, but field-independent for AB; The gate-voltage period is field-independent for CB, but proportional to 1/B for AB; Resistance stripes in the two-dimensional plane of B and gate voltage have a positive (negative) slope in the CB (AB) regime.The devices were fabr...

In this work, we demonstrate for the first time the introduction of π-complexation into a porous aromatic framework (PAF), affording significant increase in ethylene uptake capacity, as illustrated in the context of Ag(I) ion functionalized PAF-1, PAF-1-SO3Ag. IAST calculations using single-component-isotherm data and an equimolar ethylene/ethane ratio at 296 K reveal that PAF-1-SO3Ag shows exceptionally high ethylene/ethane adsorption selectivity (Sads: 27 to 125), far surpassing benchmark zeolite and any other MOF reported in literature. The formation of π-complexation between ethylene molecules and Ag(I) ions in PAF-1-SO3Ag has been evidenced by the high isosteric heats of adsorption of C2H4 and also proved by in situ IR spectroscopy studies. Transient breakthrough experiments, supported by simulations, indicate the feasibility of PAF-1-SO3Ag for producing 99.95%+ pure C2H4 in a Pressure Swing Adsorption operation. Our work herein thus suggests a new perspective to functionalizing PAFs and other types of advanced porous materials for highly selective adsorption of ethylene over ethane.

With rapid progress in DNA synthesis and sequencing, strain engineering starts to be the rate-limiting step in synthetic biology. Here, we report a gRNA-tRNA array for CRISPR-Cas9 (GTR-CRISPR) for multiplexed engineering of Saccharomyces cerevisiae . Using reported gRNAs shown to be effective, this system enables simultaneous disruption of 8 genes with 87% efficiency. We further report an accelerated Lightning GTR-CRISPR that avoids the cloning step in Escherichia coli by directly transforming the Golden Gate reaction mix to yeast. This approach enables disruption of 6 genes in 3 days with 60% efficiency using reported gRNAs and 23% using un-optimized gRNAs. Moreover, we applied the Lightning GTR-CRISPR to simplify yeast lipid networks, resulting in a 30-fold increase in free fatty acid production in 10 days using just two-round deletions of eight previously identified genes. The GTR-CRISPR should be an invaluable addition to the toolbox of synthetic biology and automation.

Contents 1. Introduction 2. Application of MFS-MOFs in catalysis 2.1 Multi-functional catalytic sites on both organic ligands and metal clusters 2.2 Multi-functional catalytic sites in organic ligands 2.3 Multi-functional catalytic sites in framework and guest moiety separately 2.4 Combine MFS of two guest components 3. Application of MFS-MOFs in gas adsorption/separation 3.1 CO 2 adsorption. 3.1.1 CO 2 adsorption by multifunctional non-metal sites. 3.1.2 CO 2 adsorption by combination of non-metal sites and open metal sites 3.2 Hydrocarbon separation. 4. Application of MFS-MOFs in optics. 5. Application of MFS-MOFs in proton conduction.