Real-time detection of DNA hybridization on microarray using a CCD-based imaging system equipped with a rotated microlens array disk

Mogi, Takeyuki; Hatakeyama, Keiichi; Taguchi, Tomoyuki; Wake, Hitoshi; Tanaami, Takeo; Hosokawa, Masahito; Tanaka, Tsuyoshi; Matsunaga, Tadashi

doi:10.1016/j.bios.2010.07.015

Cited by 19 publications

(6 citation statements)

References 14 publications

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“…Microlens arrays (MLAs), which generally refer to two-dimensional (2D) arrays of small lenses with diameter ranging from several micrometers to nearly millimeter, have diverse applications in micro-optical systems, high-definition displays, photovoltaic devices, biochemical systems, and artificial compound eyes. − In the past several years, various methods have been explored for fabricating MLAs, including thermal reflow, droplet method, hot embossing, gray scale photolithography, and so on. However, concave or negative MLAs are not availably fabricated by those well-estabilized techniques, which have extensive applications such as compound refractive lens for focusing X-rays, diffusers, and carriers of DNA chips. , Recently, some techniques have been proposed to manufacture concave MLAs.…”

Section: Introductionmentioning

confidence: 99%

Rapid Fabrication of Large-Area Concave Microlens Arrays on PDMS by a Femtosecond Laser

Yong

Yang

et al. 2013

ACS Appl. Mater. Interfaces

128

115

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A fast and single-step process is developed for the fabrication of low-cost, high-quality, and large-area concave microlens arrays (MLAs) by the high-speed line-scanning of femtosecond laser pulses. Each concave microlens can be generated by a single laser pulse, and over 2.78 million microlenses were fabricated on a 2 × 2 cm(2) polydimethylsiloxane (PDMS) sheet within 50 min, which greatly enhances the processing efficiency compared to the classical laser direct writing method. The mechanical pressure induced by the expansion of the laser-induced plasmas as well as a long resolidifing time is the reason for the formation of smooth concave spherical microstructures. We show that uniform microlenses with different diameters and depths can be controlled by adjusting the power of laser pulses. Their high-quality optical performance is also demonstrated in this work.

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Section: Introductionmentioning

confidence: 99%

Rapid Fabrication of Large-Area Concave Microlens Arrays on PDMS by a Femtosecond Laser

Yong

Yang

et al. 2013

ACS Appl. Mater. Interfaces

128

115

View full text Add to dashboard Cite

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“…When the x, y motion table is scanned at 125 mm/s, the gap can be adjusted with an accuracy of ±1.5 lm [16,17]. Figure 4 is a schematic of the system for measuring the size of the applied fluorescent paste [18,19]. It has a laser focus displacement gauge (LT-8100, Keyence Corporation), a digital imaging microscope (VHX-700F, Keyence Corporation), and three axial positioners.…”

Section: Introductionmentioning

confidence: 99%

Fabrication process of thick fluorescent layer for flat panel displays using a new paste supply system

Tsuchiya

Miyauchi

Homma

2015

Opt Rev

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A new paste and fabrication procedure for forming stable fluorescent layers on flat panel displays formation is discussed. We have characterized the uniformity and thickness of the fluorescent paste and, using simulations, have found that the optimum fabrication conditions are with a paste sectional area of 2050 lm 2 , a dispenser pressure of 0.5 MPa, a nozzle gap of 30 lm, and nozzle inner diameter of 0.14 mm. In addition, a contact angle of 0°is the optimum surface of the glass substrate, supporting a heat-treated black matrix. The finished layer has a uniform thickness of 13.9 ± 1.1 lm, and a thickness/width aspect ratio of 0.1. The new method can form uniform 8 ± 1.5-lmthick fluorescent layers at each opening in the black matrix; it reduces contamination, and can be extended to large substrates.

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“…Hardware improvements are usually made to the internal parts of the optical system. Common materials include ground glass [ 4 ], vibrating fiber [ 5 ], and rotating micro-lens [ 6 ], which are usually complex and highly demanding for the system. So, many scholars have studied how to eliminate or suppress the speckle noise of images by using image noise reduction algorithms in software.…”

Section: Introductionmentioning

confidence: 99%

Improved Weighted Non-Local Mean Filtering Algorithm for Laser Image Speckle Suppression

et al. 2022

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Laser speckle noise caused by coherence between lasers greatly influences the produced image. In order to suppress the effect of laser speckles on images, in this paper we set up a combination of a laser-structured light module and an infrared camera to acquire laser images, and propose an improved weighted non-local mean (IW-NLM) filtering method that adopts an SSI-based adaptive h-solving method to select the optimal h in the weight function. The analysis shows that the algorithm not only denoises the laser image but also smooths pixel jumps in the image, while preserving the image details. The experimental results show that compared with the original laser image, the equivalent number of looks (ENL) index of the IW-NLM filtered image improved by 0.80%. The speckle suppression index (SSI) of local images dropped from 4.69 to 2.55%. Compared with non-local mean filtering algorithms, the algorithm proposed in this paper is an improvement and provides more accurate data support for subsequent image processing analysis.

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Real-time detection of DNA hybridization on microarray using a CCD-based imaging system equipped with a rotated microlens array disk

Cited by 19 publications

References 14 publications

Rapid Fabrication of Large-Area Concave Microlens Arrays on PDMS by a Femtosecond Laser

Rapid Fabrication of Large-Area Concave Microlens Arrays on PDMS by a Femtosecond Laser

Fabrication process of thick fluorescent layer for flat panel displays using a new paste supply system

Improved Weighted Non-Local Mean Filtering Algorithm for Laser Image Speckle Suppression

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