Rapid HPLC measurement of buflomedil in plasma in poisoning cases

Forfar‐Bares, Isabelle; Péhourcq, Fabienne; Jarry, Christian

doi:10.1002/bmc.189

Cited by 4 publications

(2 citation statements)

References 13 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously published methods revealed that the analysis of clonazepam in biological and pharmaceutical samples is vitally important . Thus, several methods have been reported for the determination of this drug, including liquid–liquid extraction and SPE techniques , GC , HPLC with MS and GC–MS , and electrochemiluminescence techniques .…”

Section: Introductionmentioning

confidence: 99%

Selective extraction of clonazepam from human plasma and urine samples by molecularly imprinted polymeric beads

Panahi

Mehramizi²,

Ghassemi

2014

J of Separation Science

View full text Add to dashboard Cite

A molecularly imprinted polymer (MIP) based on free-radical polymerization was prepared with 1-(N,N-biscarboxymethyl)amino-3-allylglycerol and N,N-dimethylacrylamide as functional monomers, N,N-methylene diacrylamide as the cross-linker, copper ion-clonazepam as the template and 2,2-azobis(2-methylbutyronitrile) as the initiator. The imprinted polymer was characterized by Fourier transform infrared spectroscopy, elemental analysis, thermo-gravimetric analysis, and SEM. The MIP of agglomerated microparticles with multipores was used for SPE. The imprinted polymer sorbent was selective for clonazepam. The optimum pH and sorption capacity were 5 and 0.18 mg/g at 20C, respectively. The profile of the drug uptake by the sorbent reflects good accessibility of the active sites in the imprinted polymer sorbent. The MIP-SPE was the most feasible technique for the extraction of clonazepam with a high recovery from human plasma and urine samples.

show abstract

Section: Introductionmentioning

confidence: 99%

Selective extraction of clonazepam from human plasma and urine samples by molecularly imprinted polymeric beads

Panahi

Mehramizi²,

Ghassemi

2014

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…There have been few reports for its determination in formulation and in biological fluids using gas chromatography [2][3][4], highperformance liquid chromatography [5][6][7] and reversed-phase ionpair high-performance liquid chromatography [8,9] which mainly concerned with poising or toxicological cases. Therefore, only few methods were determined of BFM in presence of its degradation products.…”

Section: Introductionmentioning

confidence: 99%

Stability Indicating Validated Chromatographic Methods for Determination of Buflomedil in Presence of its Degradation Products

Salem¹,

Moustafa²,

Hegazy³

et al. 2013

Pharmaceut Anal Acta

View full text Add to dashboard Cite

Simple, sensitive, selective and precise stability-indicating thin-layer chromatographic (TLC) andhigh-performance liquid chromatographic (HPLC) methodsfor the determination of Buflomedil (BFM) in pharmaceutical tablets were developed and validatedas per the International Conference on Harmonizationguidelines. The TLC method employs aluminum TLCplates precoated with silica gel 60F 254 as the stationary phase and Butanol: ammonia: triethylamine (8:0.5:0.5, v/v/v) as the mobile phase to give compact spots for Buflomedil (R f =0.55) and its degradation product (R f =0.05), the chromatogram was scanned at 272 nm. The HPLC method utilizes a C18 column and amobile phase consisting of methanol: water: acetonitrile: triethylamine (50:30:20:0.4, v/v, pH 6.5) at a flow rate of 0.7 mL min -1 for the separation of Buflomedil (t R =3.76) and its degradation product (t R =2.117). Quantitation was achieved with UV detection at 272 nm. The methods were validated in terms of accuracy, precision, linearity, limits of detection, and limits of quantification. Buflomedil was exposed to acid hydrolysis and analyzed by the proposed methods in presence of its degradation products. As the methods could effectively separate the drugs from their degradation products, these techniques can be employed as stability-indicating methods that have been successively applied to pharmaceutical formulations without interference from the excipients.

show abstract

Application of a high performance liquid chromatography–tandem mass spectrometry method for determination of buflomedil in human plasma for a bioequivalence study

Ren

Yang

Peng

et al. 2013

Journal of Chromatography B

View full text Add to dashboard Cite

Rapid HPLC measurement of buflomedil in plasma in poisoning cases

Cited by 4 publications

References 13 publications

Selective extraction of clonazepam from human plasma and urine samples by molecularly imprinted polymeric beads

Selective extraction of clonazepam from human plasma and urine samples by molecularly imprinted polymeric beads

Stability Indicating Validated Chromatographic Methods for Determination of Buflomedil in Presence of its Degradation Products

Application of a high performance liquid chromatography–tandem mass spectrometry method for determination of buflomedil in human plasma for a bioequivalence study

Contact Info

Product

Resources

About