Application of a high performance liquid chromatography–tandem mass spectrometry method for determination of buflomedil in human plasma for a bioequivalence study

Ren, Li; Yang, Chun; Peng, Yan; Fan, Li; Li, Yinghui; Zheng, Huimin

doi:10.1016/j.jchromb.2013.07.010

Cited by 2 publications

(3 citation statements)

References 6 publications

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“…Serum samples from humans were collected to determine succinate using HPLC as previously described [ 69 ]. Mice samples were collected under CO 2 euthanasia, while human samples were collected from conscious individuals.…”

Section: Methodsmentioning

confidence: 99%

Succinate causes pathological cardiomyocyte hypertrophy through GPR91 activation

et al. 2014

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BackgroundSuccinate is an intermediate of the citric acid cycle as well as an extracellular circulating molecule, whose receptor, G protein-coupled receptor-91 (GPR91), was recently identified and characterized in several tissues, including heart. Because some pathological conditions such as ischemia increase succinate blood levels, we investigated the role of this metabolite during a heart ischemic event, using human and rodent models.ResultsWe found that succinate causes cardiac hypertrophy in a GPR91 dependent manner. GPR91 activation triggers the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), the expression of calcium/calmodulin dependent protein kinase IIδ (CaMKIIδ) and the translocation of histone deacetylase 5 (HDAC5) into the cytoplasm, which are hypertrophic-signaling events. Furthermore, we found that serum levels of succinate are increased in patients with cardiac hypertrophy associated with acute and chronic ischemic diseases.ConclusionsThese results show for the first time that succinate plays an important role in cardiomyocyte hypertrophy through GPR91 activation, and extend our understanding of how ischemia can induce hypertrophic cardiomyopathy.Electronic supplementary materialThe online version of this article (doi:10.1186/s12964-014-0078-2) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Succinate causes pathological cardiomyocyte hypertrophy through GPR91 activation

et al. 2014

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“…The precursor ions (Q1) for each of the 11 compounds were obtained from the exact mass of their protonated molecular ions [M+H] + . One to three product ions (Q3) were identified based on the MS information obtained from LC-QTOF-MS (Table 1) and various references [7][8][9][10][11][12][13][14][15][16][17]. Several distinguishable product ions that had fragmented from each precursor ion were applied to the MRM transitions and optimized for LC-MS/MS (Table 2).…”

Section: Optimization Of the Lc-esi-ms/ms Methodsmentioning

confidence: 99%

“…Therefore, the rapid screening and identification of adulterating compounds in dietary supplements is necessary for public safety. Several analytical studies on adulterated compounds have been conducted using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) [7][8][9][10][11][12][13][14][15][16][17]. However, in most previous studies, a single or small number of compounds were analyzed simultaneously in biological matrices such as blood, plasma, and urine, but not in food matrices.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection and analysis of 11 nootropic compounds in 54 samples via liquid chromatography‐electrospray ionization‐tandem mass spectrometry and quadrupole time‐of‐flight mass spectrometry

Kim,

Choi,

Shin

et al. 2023

Separation Science Plus

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Dietary supplements used for improving brain function and strengthening attention are increasingly popular. However, illegal dietary supplements containing unapproved drugs continue to be seized. Therefore, it is necessary to rapidly screen and identify adulterated compounds in dietary supplements for public safety. To develop the simultaneous analytical method, we selected 11 nootropic compounds and optimized the simultaneous liquid chromatography‐electrospray ionization‐tandem mass spectrometry and optimal conditions for liquid chromatography‐quadrupole time‐of‐flight mass spectrometry were proposed to determine the fragmentation patterns of each compound. The specificity, linearity, limit of detection, limit of quantification, method detection limit, method quantitation limit, precision, accuracy, matrix effect, and stability of this method were evaluated according to international validation guidelines. The validated methods were applied to 54 samples containing dietary supplements and seized samples. On screening 11 nootropic compounds were detected in four solid samples (tablet and powder). By comparing the product ion ratios with the standards and quantification, vinpocetine (6.52–34.2 mg/g) was found in three samples, and omberacetam (995.6 mg/g) in one sample. To re‐confirm the detected compounds, the fragmentation patterns were compared with those of the standards using liquid chromatography‐quadrupole time‐of‐flight mass spectrometry. The developed simultaneous analysis method can help maintain public health and safety.

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Application of a high performance liquid chromatography–tandem mass spectrometry method for determination of buflomedil in human plasma for a bioequivalence study

Cited by 2 publications

References 6 publications

Succinate causes pathological cardiomyocyte hypertrophy through GPR91 activation

Succinate causes pathological cardiomyocyte hypertrophy through GPR91 activation

Simultaneous detection and analysis of 11 nootropic compounds in 54 samples via liquid chromatography‐electrospray ionization‐tandem mass spectrometry and quadrupole time‐of‐flight mass spectrometry

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