Rabbit Urinary Bladder Blood Flow Changes During the Initial Stage of Partial Outlet Obstruction

Lieb, Jeremy; Chichester, Paul; Kogan, Barry A.; Das, Anupam; Leggett, Robert E.; Schröder, Annette; Levin, Robert M.

doi:10.1016/s0022-5347(05)67205-9

Cited by 37 publications

(18 citation statements)

References 38 publications

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“…Previous studies (14) demonstrated that PBOO resulted in a significant NOstimulated increase in blood flow at 1 day, which returned to control levels at 3 and 7 days postobstruction. This increase in blood flow would be expected to compensate for the ischemia mediated by the obstruction.…”

Section: Discussionmentioning

confidence: 78%

l-NAME, a nitric oxide synthase inhibitor, diminishes oxidative damage in urinary bladder partial outlet obstruction

Conners

Whitebeck

Chicester

et al. 2006

American Journal of Physiology-Renal Physiology

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Partial bladder outlet obstruction (PBOO) results in cellular damage due to ischemia and reperfusion injury. Our study seeks to establish how early this damage can occur and the role that nitric oxide may play in its pathophysiology. Surgical PBOO (1, 3, and 7 days) were performed on male New Zealand White rabbits. Half of the animals were premedicated for 3 days with N(G)-nitro-l-arginine methyl ester(l-NAME), an inhibitor of nitric oxide synthase before obstruction. Bladder weight increased with duration of PBOO but was significantly lower at 3 and 7 days in animals treated with l-NAME compared with their untreated counterparts. Contractile function decreased progressively with PBOO duration. At 1 day postobstruction, bladder contractility was significantly lower in the l-NAME rabbits than in the untreated rabbits. At 3 and 7 days, contractility of the l-NAME bladders was equal or higher than the untreated bladders. The level of hypoxia at 1 day after obstruction was significantly higher in the l-NAME-treated animals than in the untreated controls but equal at 3 and 7 days obstruction. Increased nitrotyrosine was seen by Western blot in all obstructed animals. However, the amount was significantly less in the l-NAME-treated animals at 3 and especially at 7 days. Nerve density decreased progressively after obstruction; however, it decreased to a significantly lesser degree in the l-NAME-treated bladders than in the untreated groups. These results suggest that l-NAME pretreatment enhanced ischemic damage at 1 day after obstruction but protected the bladder from nitric oxide-generated free radical damage at the later time periods by inhibiting the generation of nitrotyrosine.

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Section: Discussionmentioning

confidence: 78%

l-NAME, a nitric oxide synthase inhibitor, diminishes oxidative damage in urinary bladder partial outlet obstruction

Conners

Whitebeck

Chicester

et al. 2006

American Journal of Physiology-Renal Physiology

Self Cite

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“…In that first study, there were significant differences between muscle and mucosa. We believe this is because the mucosa has a significantly higher blood flow than the muscle, a significantly higher metabolic rate than the muscle and is significantly more sensitive to ischemia/reperfusion; and thus even mild obstruction would lead to oxidative damage to the mucosa, but not the muscle.…”

Section: Discussionmentioning

confidence: 99%

Effect of severity and duration of bladder outlet obstruction on catalase and superoxide dismutase activity

et al. 2013

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Abbreviations & AcronymsObjectives: To study the effects of partial bladder outlet obstruction on the cell's anti-oxidant defense mechanisms, superoxide dismutase and catalase, in order to elucidate how the bladder responds to oxidative stress. Methods: Four groups of eight rabbits were subjected to partial bladder outlet obstruction for 4, 8 and 12 weeks. Eight sham rabbits were used as the control group. The bladders were removed under anesthesia, and the muscle and mucosa were separated, frozen and stored at -80°C for analysis. Superoxide dismutase and catalase assays were carried out on these tissues. The groups were also categorized by severity (mild, intermediate and severe) of decompensation, as well as duration. Results: When separated by duration, catalase activity of the mucosa was significantly higher in the control and the 12-weeks obstructed rabbits. This activity was lower than the control in the 4-and 8-weeks obstructed rabbits. When separated by severity, catalase activity of the mucosa was significantly higher and severely decompensated than the muscle in the controls. When separated by duration or severity, superoxide dismutase activity of the muscle was significantly lower than controls for all obstructed rabbits. The activities of both superoxide dismutase and catalase were significantly reduced in the severely decompensated bladder smooth muscle, but not in the 12-weeks obstructed bladder smooth muscle. Conclusions: Partial bladder outlet obstruction has significant effects on the activity of both superoxide dismutase and catalase in the bladder, with variations that are dependent on the severity and duration of the obstruction.

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“…Several studies used rats (3, 4), mice (1, 10, 11), rabbits (12), guinea pigs (13) and pigs (14) to mimic BOO, whose structural and physiological changes of the bladder wall are similar to those observed in men suffering from BPH (9). …”

Section: Discussionmentioning

confidence: 99%

“…In fact, mice 1 week after obstruction already have a rapid increase in bladder mass, which worsened 3 and 5 weeks after the surgical procedure (11). Similarly, rats and rabbits have increased blood flow after 24 hours of obstruction, which could be the first stimulus for hypertrophy (12, 15). This event occurs due to stretch of the bladder wall components, leading to thickening of epithelium, muscle layer and serosa (16) and increase in synthesis and deposition of collagen (17).…”

Section: Discussionmentioning

confidence: 99%

Effects of nitric oxide inhibitors in mice with bladder outlet obstruction

et al. 2017

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Purpose To investigate the lower urinary tract changes in mice treated with L-NAME, a non-selective competitive inhibitor of nitric oxide synthase (NOS), or aminoguanidine, a competitive inhibitor of inducible nitric oxide synthase (iNOS), after 5 weeks of partial bladder outlet obstruction (BOO), in order to evaluate the role of constitutive and non-constitutive NOS in the pathogenesis of this experimental condition.Materials and Methods C57BL6 male mice were partially obstructed and randomly allocated into 6 groups: Sham, Sham + L-NAME, Sham + aminoguanidine, BOO, BOO + L-NAME and BOO + aminoguanidine. After 5 weeks, bladder weight was obtained and cystometry and tissue bath contractile studies were performed.Results BOO animals showed increase of non-voiding contractions (NVC) and bladder capacity, and also less contractile response to Carbachol and Electric Field Stimulation. Inhibition of NOS isoforms improved bladder capacity and compliance in BOO animals. L-NAME caused more NVC, prevented bladder weight gain and leaded to augmented contractile responses at muscarinic and electric stimulation. Aminoguanidine diminished NVC, but did not avoid bladder weight gain in BOO animals and did not improve contractile responses.Conclusion It can be hypothesized that chronic inhibition of three NOS isoforms in BOO animals leaded to worsening of bladder function, while selective inhibition of iNOS did not improve responses, what suggests that, in BOO animals, alterations are related to constitutive NOS.

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Rabbit Urinary Bladder Blood Flow Changes During the Initial Stage of Partial Outlet Obstruction

Cited by 37 publications

References 38 publications

l-NAME, a nitric oxide synthase inhibitor, diminishes oxidative damage in urinary bladder partial outlet obstruction

l-NAME, a nitric oxide synthase inhibitor, diminishes oxidative damage in urinary bladder partial outlet obstruction

Effect of severity and duration of bladder outlet obstruction on catalase and superoxide dismutase activity

Effects of nitric oxide inhibitors in mice with bladder outlet obstruction

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