Effect of severity and duration of bladder outlet obstruction on catalase and superoxide dismutase activity

Abstract

Abbreviations & AcronymsObjectives: To study the effects of partial bladder outlet obstruction on the cell's anti-oxidant defense mechanisms, superoxide dismutase and catalase, in order to elucidate how the bladder responds to oxidative stress. Methods: Four groups of eight rabbits were subjected to partial bladder outlet obstruction for 4, 8 and 12 weeks. Eight sham rabbits were used as the control group. The bladders were removed under anesthesia, and the muscle and mucosa were separated, frozen and stored at -80°C for analysis. Superoxide dismutase and catalase assays were carried out on these tissues. The groups were also categorized by severity (mild, intermediate and severe) of decompensation, as well as duration. Results: When separated by duration, catalase activity of the mucosa was significantly higher in the control and the 12-weeks obstructed rabbits. This activity was lower than the control in the 4-and 8-weeks obstructed rabbits. When separated by severity, catalase activity of the mucosa was significantly higher and severely decompensated than the muscle in the controls. When separated by duration or severity, superoxide dismutase activity of the muscle was significantly lower than controls for all obstructed rabbits. The activities of both superoxide dismutase and catalase were significantly reduced in the severely decompensated bladder smooth muscle, but not in the 12-weeks obstructed bladder smooth muscle. Conclusions: Partial bladder outlet obstruction has significant effects on the activity of both superoxide dismutase and catalase in the bladder, with variations that are dependent on the severity and duration of the obstruction.

Sulforaphane Ameliorates Bladder Dysfunction through Activation of the Nrf2‐ARE Pathway in a Rat Model of Partial Bladder Outlet Obstruction

“…The activities of redox status markers (total SOD, GSH-Px, and CAT) were also measured. We found that the activities of these markers were significantly decreased in the bladder of the BOO group compared to the sham group; however, SFN significantly enhanced their activities in BOO rats, which is consistent with other literature [ 18 ]. These results suggested that SFN could alleviate oxidative stress by increasing the activities of antioxidative enzymes in BOO rats.…”

“…The oxidative stress marker was conducted as previously described [ 17 , 18 ]. The content of MDA, GSH-Px, total SOD, and CAT in the bladder tissues was measured by spectrophotometry according to the manufacturer's protocols.…”

Effects of Resveratrol on Methotrexate‐Induced Testicular Damage in Rats

“…SOD is one of the major antioxidant enzymes that protect the male reproductive organs against the harmful effects of ROS [27]. Hydrogen peroxide (H 2 O 2 ) , less effective than the superoxide group, is neutralized by being converted into products with a weaker effect, such as water and oxygen, by enzymes present in tissue, such as catalase and glutathione [28]. We investigated whether the antioxidant enzymes SOD and CAT have a protective effect that eliminates free radicals forming with MTX.…”

The relevance of immune responses to partial bladder outlet obstruction and reversal

“…A previous study indicated that PBOO significantly increased a variety of oxidative stress factors, including the levels of 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) in the urine, plasma, and bladder tissue and the level of malondialdehyde (MDA) in plasma . In contrast, the activity of both superoxide dismutase and catalase in the bladder is decreased after obstruction, suggesting that PBOO compromises the antioxidative capacity of the tissue . Various animal models of PBOO indicate that PBOO is intimately associated with inflammation and oxidative stress, implying a critical role of the systemic immune response in the pathogenesis of PBOO .…”

“…4 In contrast, the activity of both superoxide dismutase and catalase in the bladder is decreased after obstruction, suggesting that PBOO compromises the antioxidative capacity of the tissue. 5 Various animal models of PBOO indicate that PBOO is intimately associated with inflammation and oxidative stress, implying a critical role of the systemic immune response in the pathogenesis of PBOO. 6,7 In addition, another study demonstrated a transient, reversible increase in the levels of circulating myeloid-derived suppressor cells (MDSCs), interleukin (IL)-10, and interferon (IFN)-γ in a rat model of PBOO.…”