Sulforaphane Ameliorates Bladder Dysfunction through Activation of the Nrf2‐ARE Pathway in a Rat Model of Partial Bladder Outlet Obstruction

Abstract

Purpose. We evaluated the effect of sulforaphane (SFN) treatment on the function and changes of expression of Nrf2-ARE pathway in the bladder of rats with bladder outlet obstruction (BOO). Materials and Methods. A total of 18 male Sprague-Dawley rats at age of 8 weeks were divided into 3 groups (6 of each): the sham operated group, the BOO group, and the BOO+SFN group. We examined histological alterations and the changes of oxidative stress markers and the protein expression of the Nrf2-ARE pathway. Results. We found that SFN treatment could prolong micturition interval and increase bladder capacity and bladder compliance. However, the peak voiding pressure was lower than BOO group. SFN treatment can ameliorate the increase of collagen fibers induced by obstruction. SFN treatment also increased the activity of SOD, GSH-Px, and CAT compared to the other groups. The level of bladder cell apoptosis was decreased in BOO rats with SFN treatment. Moreover, SFN could reduce the ratio of Bax/Bcl-2 expression. Furthermore, SFN could activate the Nrf2 expression with elevation of its target antioxidant proteins. Conclusions. The sulforaphane-mediated decrease of oxidative stress and activation of the Nrf2-ARE pathway may ameliorate bladder dysfunction caused by bladder outlet obstruction.

Re: effects of silodosin, a selective alpha-1a adrenoceptor antagonist, on erectile function in a rat model of partial bladder outlet obstruction

“…In rat model of BOO, increased oxidative stress and activation of transcription factors in bladder wall have been reported. 6,7 Similar changes may be seen in penises of rats. This interaction results in endothelial dysfunction, because of decreased bioavailability of nitric oxide (NO), due to the reaction between NO and ROS.…”

“…It would be better if eNOS expression studied in penile tissue. In rat model of BOO, increased oxidative stress and activation of transcription factors in bladder wall have been reported . Similar changes may be seen in penises of rats.…”

Sulforaphane Treatment of Stress Urinary Incontinence Via the Nrf2-ARE Pathway in a Rat Model

“…The cystometric analysis was performed as previously described by Liu et al [26]. Continuous cystometry was performed in conscious rats in a metabolic cage without restraint.…”

The Glucosinolates, A Sulphur Glucoside Family of Mustard Phytochemicals With Diverse Biomedical Application

“…Suppression of NLRP3 inflammasome alleviating acute gouty inflammation [147] Modification of Histone H3, unpacking of chromatin, to prime defence [104] Nrf2-Inducers Counteract Neurodegeneration in Friedreich's Ataxia [148] Modulation of oxidative stress and inflammation in rats with toxic hepatitis [149] Modulation of oxidative damage in lead exposed rat hippocampus [150] Prevention of dexamethasone-induced myotube atrophy via Akt/Foxo1 [151] Induction of p53 deficient SW480 cell apoptosis by ROS MAPK signalling [152] Role of microRNAs in the chemo preventive activity of SFN [153] Up regulation of Nrf2 protection in doxorubicin-induced chronic heart failure [154] Increased Nrf2 expression protects alveolar epithelial cells against oxidative injury [155] Novel phosphonate analogs of SFN with in vitro and in vivo anticancer activity [156] Inhibition of PDGF-induced vascular SMC proliferation by targeting mTOR/p70S6kinase signalling independently of Nrf2 activation [157] Gastrointestinal protection against H. pylori and NSAID-Induced Oxidative Stress [158] Protection from cerebral ischemic/reperfusion injury via inhibition of NLRP3 inflammasome activation in rats [159] Protection against sodium valproate-induced acute liver injury [160] Enhanced SFN cardioprotection against oxidative stress by 17β-Estradiol [160] Photoprotective Effects of SFN and Hispidulin [106] Differential modulation of mitochondrial biogenesis/dynamics in normal and tumour cells [161] Nrf2 targeting by SFN: A potential therapy for cancer treatment [162,163] Improvement of neuronal mitochondrial function in brain tissue [164] Protection of pancreatic Acinar cell injury by modulating Nrf2-mediated oxidative stress and the NLRP3 inflammatory pathway [165] Improvement in chemotherapy efficacy targeting cancer stem cell-like properties [166] Protection against rotenone-induced neurotoxicity via mTOR, Nrf2, and autophagy [167] Chemoprevention of oxidative stress-associated with oral carcinogenesis [168] Amelioration of bladder dysfunction via activation of Nrf2-ARE Pathway [169] Broccoli sprout homogenate treatment for Sickle Cell Disease …”

“…Leukemia [87,124,[175][176][177][178][179] Prostate cancer [91,95,[180][181][182] non-small cell lung cancer cells [107,183,184] Pancreatic cancer [165,[185][186][187] Breast cancer [92, 93, 101-103, 105, 108, 109, 188-195] Bladder cancer [169,[196][197][198][199][200][201] Ovarian cancer [99] HepG2 Carcinoma Cells [202][203][204][205][206] Gastric cancer [207,208] Squamous cell carcinoma [209,210] Nasopharangeal cancer [211] Melanoma [212] Glioma [173,[213][214][215] Colon cancer [110,216,217] Lung cancer [218,219]...…”