Quantificação de células CD34+ do sangue do cordão umbilical de cães

Nakage, Ana Paula Massae; Santana, Áureo Evangelista; Cápua, Maria Luísa Buffo de; Godoy, Aline Vieira

doi:10.1590/s0103-84782008005000057

Cited by 3 publications

(5 citation statements)

References 42 publications

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“…Thus, this cellular type is being widely used in researches because they do not present ethical barriers due to the ease of obtaining them and still to be used in autologous transplants, less probability of immunological rejection and the need of cellular inventory, since they are apparently inexhaustible (Castro-Silva et al 2010). Nevertheless, as previous cited, MSCs have the potential to undergo to differentiation into cells of the mesodermal lineage, such as bone, fat and cartilage, and have the potential to differentiate into cells of the endodermal and ectodermal linage (Nakage et al 2009, Pinto Filho et al 2013). …”

Section: Mesenchymal Stem Cellmentioning

confidence: 77%

“…In dogs, the presence of HSCs have been observed mostly in bone marrow and umbilical cord blood, being used in cell therapy for neoplasia, hematological, metabolic, and immunological diseases (Bruno et al 2009, Nakage et al 2009, Ryu et al 2012, Bittencourt et al 2016, Stolfi et al 2016.…”

Section: Hematopoietic Stem Cellsmentioning

confidence: 99%

“…MSCs have characteristics of high renewal power and differentiation in several cell lineages, the cell lines of the mesoderm that MSCs can originate are adipocytes, osteocytes, chondrocytes, and connective stroma cells, which demonstrates their high plasticity (Pereira et al 2008, Nakage et al 2009, Castro-Silva et al 2010, Monteiro et al 2010, Tharasanit et al 2011). Thus, the MSCs have been use to recover damaged organs and tissues by introducing them after a differentiation induction into the compromised places and restoring the lost function (Narazaki & Cristante 2011).…”

Section: Mesenchymal Stem Cellmentioning

confidence: 99%

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Bone marrow stem cell applied in the canine veterinary clinics

Gomes

Oliveira²,

Pinheiro

et al. 2017

Pesq. Vet. Bras.

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Cell therapy represents an old therapeutic practice initiated with the transfusion of whole blood in different clinical situations. There is now a breakthrough in the study of multipotent stem cell therapy because of its functionality in regeneration of tissues, which promotes attention of the scientific community. Bone marrow is one of the main sources of multipotent stem cells, composed by hematopoietic stem cells responsible for the renewal of the cellular components of the blood, and mesenchymal stem cells that aid in the regeneration of tissues. These cells have a strong potential for the treatment of several diseases, due their main characteristics such as high plasticity, capacity for self-renewal and immunomodulation. Although, there are many studies that show good results with the use of cell therapy as a form of treatment for several diseases, some studies still show inconclusive or unsatisfactory results. Therefore, the objective of this study was to review the application of bone marrow stem cells in the canine model since improvements on the knowledge of the technique are necessary to enable its applicability with safety and efficacy.

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Section: Mesenchymal Stem Cellmentioning

confidence: 77%

Section: Hematopoietic Stem Cellsmentioning

confidence: 99%

Section: Mesenchymal Stem Cellmentioning

confidence: 99%

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Bone marrow stem cell applied in the canine veterinary clinics

Gomes

Oliveira²,

Pinheiro

et al. 2017

Pesq. Vet. Bras.

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“…Na prática mé-dico-veterinária essa técnica tem sido utilizada para avaliar a progressão das infecções pelos vírus da Imunodeficiência Felina e da Leucemia Felina, na resposta de transplantes de órgãos, em modelos experimentais caninos, bem como na resposta imune a infecções (Byrne et al 2000, Dias 2003, Nakage & Santana 2006, Nakage et al 2009). Anticorpos monoclonais que reconhecem linfócitos têm sido úteis no diagnóstico e avaliação da imunodeficiência, na terapia imunossupressora em transplantes de órgãos e doenças imunes, e na quimioterapia de leucemias e linfomas (Rivas et al 1996, Coleta 2009, Miotto 2009).…”

Section: Introductionunclassified

Quantificação de subpopulações linfocitárias no sangue periférico de cães sadios adultos

et al. 2017

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RESUMO: A citometria de fluxo vem se firmando como uma ferramenta útil na prática médico-veterinária, particularmente na clínica de pequenos animais. Tal conhecimento tem ensejado o estabelecimento de valores fisiológicos para diferentes subpopulações linfocitárias, indispensáveis à compreensão da dinâmica da celularidade linfóide, em diversas situações patológicas. Assim sendo, o presente ensaio teve como objetivo imunomarcar, por intermédio da técnica citométrica, as subpopulações linfocitárias CD5+CD4+, CD5+CD8+ e CD21+, em quatro diferentes raças de cães domésticos e sadios, de tal forma a agregar informações sobre o perfil imunológico das diferentes raças. Foram utilizados 40 cães adultos (2-7 anos), machos e fêmeas, das raças Beagle (G1, n=10), Golden Retriever (G2, n=10), Bulldog Inglês (G3, n=10) e sem raça definida (SRD) (G4, n=10). As colheitas de sangue foram realizadas por venipunção jugular, utilizando-se sistema de frascos a vácuo (K2-EDTA). O hemograma e o processamento das amostras para citometria de fluxo foram realizados num prazo máximo de 24 horas após a colheita do sangue. As amostras foram analisadas no citofluorômetro FACSCANTO (Becton Dickinson, San Jose, CA, USA). Utilizou-se o programa FACSDiva (Becton Dickinson, San Jose, CA, USA), para identificar e quantificar as células CD5+CD4+, CD5+CD8+ e CD21+, que forneceu o histograma e respectiva tabela com a quantidade de células detectadas pela imunofenotipagem. Os dados obtidos para contagem de linfócitos T auxiliares, T citotóxicos/supressores e linfócitos B foram tabulados e submetidos a Análise de Variância pelo teste F. O teste de Tukey a 5% de probabilidade foi utilizado para comparação das médias entre as diferentes raças de cães. Os valores médios de contagens de células CD5+CD8+ no sangue periférico do G1, G2, G3 e G4 foram de 155, 206, 544 e 503 células/μL, respectivamente. Os valores médios de contagens de células CD5+CD4+ no sangue periférico do G1, G2, G3 e G4 foram de 746, 642, 1101 e 855 células/μL, respectivamente. Os valores médios de contagens de células CD21+ no sangue periférico do G1, G2, G3 e G4 foram de 171, 299, 494 e 403 células/μL, respectivamente. Assim sendo, o número médio de células obtido para a subpopulação de linfócitos T citotóxicos foi significativamente maior (p<0,05) nos Bulldogs Ingleses e cães SRD, comparativamente aqueles encontrados nos grupos de Beagles e Golden Retrievers. Ademais, observou-se que o número médio de células obtido para a subpopulação de linfócitos B foi significavamente maior (p<0,05) nos Bulldogs Ingleses, quando comparado àquele dos Beagles.

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“…There are reports about the hematological values, including the reticulocytes counts, on which the quantity found in the UCB has presented to be higher that that from the peripheral blood of neonate and adult dogs (Nakage, 2002(Nakage, , 2003. There are also reports on the influence of the type of parturition over the hematological values (Nakage, 2005).…”

Section: Introductionmentioning

confidence: 99%

T lymphocyte subsets of the umbilical cord blood of dogs

Cápua

Santana

Nakage

et al. 2009

Arq. Bras. Med. Vet. Zootec.

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The hematological parameters red blood cells (RBC) and total white blood cells (WBC) counts, hematocrit, hemoglobin concentration, and RBC indexes (median corpuscular volume and median corpuscular hemoglobin concentration) were determined and T CD5 + lymphocytes and CD4 + and CD8 + subpopulations of the umbilical cord blood (UCB) of dogs were quantified by the cytofluorimetric technique. Nine adult Beagles, from two do five-year old, were used as control. The umbilical cord blood (UCB) was collected from 20 neonate dogs. The method for the UCB collection was adequate to obtain sufficient quantity of blood for the accomplishment of the hematological analyses and lymphocyte quantification. Cytoscopic preparations of the UCB suggested high erythropoietic activity. There was no difference for the global leukocyte and lymphocyte counts between the groups. UCB T lymphocyte counts were lower than those obtained for adult dogs. The proportion of CD4:CD8 showed a great dominance of T CD4 + cells over T CD8 + lymphocytes in UCB.

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Quantificação de células CD34+ do sangue do cordão umbilical de cães

Abstract: Stem cells promote the reconstitution of the

Cited by 3 publications

References 42 publications

Bone marrow stem cell applied in the canine veterinary clinics

Bone marrow stem cell applied in the canine veterinary clinics

Quantificação de subpopulações linfocitárias no sangue periférico de cães sadios adultos

T lymphocyte subsets of the umbilical cord blood of dogs

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