T lymphocyte subsets of the umbilical cord blood of dogs

Abstract

The hematological parameters red blood cells (RBC) and total white blood cells (WBC) counts, hematocrit, hemoglobin concentration, and RBC indexes (median corpuscular volume and median corpuscular hemoglobin concentration) were determined and T CD5 + lymphocytes and CD4 + and CD8 + subpopulations of the umbilical cord blood (UCB) of dogs were quantified by the cytofluorimetric technique. Nine adult Beagles, from two do five-year old, were used as control. The umbilical cord blood (UCB) was collected from 20 neonate dogs. The method for the UCB collection was adequate to obtain sufficient quantity of blood for the accomplishment of the hematological analyses and lymphocyte quantification. Cytoscopic preparations of the UCB suggested high erythropoietic activity. There was no difference for the global leukocyte and lymphocyte counts between the groups. UCB T lymphocyte counts were lower than those obtained for adult dogs. The proportion of CD4:CD8 showed a great dominance of T CD4 + cells over T CD8 + lymphocytes in UCB.

Morphological characterization of the progenitor blood cells in canine and feline umbilical cord

“…The umbilical cord blood (UCB) is an important source of hematopoietic stem cells with great deal of interest in regenerative medicine (Capua et al,2009; Erices et al,2000; Gluckman,2000; Hows,2001; Huss,2000; Lee et al,2004; Mareschi et al,2001; Romanov et al,2003). In stem cell transplants derived from UCB, the T lymphocytes are almost totally excluded from the sample, reducing the risk for rejection (Capua et al,2009; Gluckman,2000; Hows,2001; Huss,2000). Moreover, the cord blood cells can be accessed without affecting the fetus or the mother during the birth (Huss,2000).…”

“…Differences between CD34+ cells derived from UCB and bone marrow were reported (Barker and Wagner,2003; Mareschi et al,2001). The proliferative capacity and number of colony‐forming units related to granulocytes, erythrocytes, monocytes, and megakaryocytes of CD34+ shown to be reasonably high in UCB than the peripheral blood of adult animals (Berenson et al,1991; Capua et al,2009; Mitchell et al,1997; Romanov et al,2003). Studies also reported phenotypical differences of the surfaces markers of CD34+ derived from the bone marrow and UCB (Barker and Wagner,2003; Berenson et al,1991).…”