Production of Taxol® and baccatin III by a selected Taxus baccata callus line and its derived cell suspension culture

Cusidó, Rosa M.; Palazón, Javier; Navia-Osorio, Alberto; Mallol, Anna; Bonfill, Mercedes; Morales, C; Piñol, M. Teresa

doi:10.1016/s0168-9452(99)00093-x

Cited by 70 publications

(35 citation statements)

References 24 publications

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“…By contrast, higher levels of Lphenylalanine did not improve the accumulation of scopoletin. Similar results on the inclusion of L-phenylalanine in the medium on cell culture of different plants were reported previously (Cusidó et al 1999) (Brincat et al 2002). The addition of 0.2 Mm/L of L-phenylalanine to cell culture of Cistanche deserticola resulted in 75% higher production of phenylethanoid glycosides, compared with the cell culture without precursors (Ouyang et al 2005).…”

Section: Discussionsupporting

confidence: 75%

Enhanced Accumulation of Scopoletin in Cell Suspension Culture of Spilanthes acmella Murr. Using Precursor Feeding

Abyari

Nasr

Soorni

et al. 2016

Braz. arch. biol. technol.

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Section: Discussionsupporting

confidence: 75%

Enhanced Accumulation of Scopoletin in Cell Suspension Culture of Spilanthes acmella Murr. Using Precursor Feeding

Abyari

Nasr

Soorni

et al. 2016

Braz. arch. biol. technol.

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“…The production of high yield of secondary compounds has also been reported from callus culture, from suspension culture or by using precursor (Cusido et al 1999, Croteau et al 2000, Jordan et al 2006.…”

Section: Resultsmentioning

confidence: 95%

Assessment of Secondary Metabolites in In vitro Regenerated Plantlets of Oroxylum indicum (L.) Vent

Gokhale¹,

Bansal²

2010

Plant Tissue Cult. & Biotech.

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Flavonoids baicalein and baicalein-7-o-glucoside were estimated in in vitro raised (six months old) as well as nature grown (12 months old) plants of Oroxylum indicum (L.) Vent. Multiple shoot formation and elongation were obtained from axillary and apical bud explants of O. indicum on MS containing BAP (1 mg/l) and AgNO3 (2 mg/l). Rooting of the regenerated shoots was achieved on medium (half strength) containing IBA (1 mg/l) and AgNO3 (1 mg/l). A quantitative HPLC analysis of methanolic extract of leaf and root indicated the enhancement of baicalein-7-o glucoside in in vitro raised root and leaf of Oroxylum indicum.

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“…Taxanes were extracted from lyophilized cells and the culture medium as described by Cusidó et al (1999). Quantification of paclitaxel and baccatin III was performed according to Grothaus et al (1995) using an indirect competitive inhibition enzyme immunoassay (CIEIA).…”

Section: Paclitaxel and Baccatin III Measurementsmentioning

confidence: 99%

Effects of immobilization by entrapment in alginate and scale‐up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata

Bentebibel

Moyano

Palazón

et al. 2005

Biotech & Bioengineering

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101

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Paclitaxel and baccatin III-producing cells of Taxus baccata were immobilized within Ca(2+)-alginate beads. Under established optimum conditions for the biosynthesis of both taxanes, the yields of paclitaxel and baccatin III in shake-flask cultures of free cells increased by factors of up to 3 and 2, respectively, in the corresponding cultures of immobilized cells. Although the scale-up from shake-flask to bioreactor culture usually results in reduced productivities when both free and immobilized cells were grown in the same optimum conditions in three different bioreactor types (Stirred, Airlift, and Wave) running for 24 days in a batch mode and with the system optimized in each case, there was a considerable increase in the yields of paclitaxel and baccatin III. Among the reactors, the Stirred bioreactor was the most efficient in promoting immobilized cell production of paclitaxel, giving a content of 43.43 mg.L(-1) at 16 days of culture, equivalent to a rate of 2.71 mg.L(-1).day(-1). To our knowledge, the paclitaxel productivity obtained in this study is one of the highest reported so far by academic laboratories for Taxus species cultures in bioreactors.

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Production of Taxol® and baccatin III by a selected Taxus baccata callus line and its derived cell suspension culture

Cited by 70 publications

References 24 publications

Enhanced Accumulation of Scopoletin in Cell Suspension Culture of Spilanthes acmella Murr. Using Precursor Feeding

Enhanced Accumulation of Scopoletin in Cell Suspension Culture of Spilanthes acmella Murr. Using Precursor Feeding

Assessment of Secondary Metabolites in In vitro Regenerated Plantlets of Oroxylum indicum (L.) Vent

Effects of immobilization by entrapment in alginate and scale‐up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata

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