Enhanced Accumulation of Scopoletin in Cell Suspension Culture of Spilanthes acmella Murr. Using Precursor Feeding

Abyari, Mohammad; Nasr, Nasrin; Soorni, Jahad; Sadhu, Deokule

doi:10.1590/1678-4324-2016150533

Cited by 22 publications

(9 citation statements)

References 22 publications

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“…Enhanced trigonelline content was noticed through thin-layer chromatography, gas–liquid chromatography, and GC–MS analysis in nicotinic acid used as precursor in in vitro callus culture ( Mathur and Kamal, 2012 ). Similarly, casein hydrolysate precursor feeding in cell suspension culture at 50, 75, and 100 mg/L concentrations resulted in the highest scopoletin compound, as revealed by high-performance thin-layer chromatography analysis ( Abyari et al, 2016 ). Various explants of Simmondsia chinensis (leaves, nodes, internodes, shoot apices, and cotyledons) were used for callus induction from MS media along with various growth regulators [NAA, BAP, indole-3-acetic acid (IAA), and isopentenyl adenine (2ip)], and the final quantitative estimation of essential oils was observed at the highest amount of oil content at a 9-week-old callus culture ( Aftab et al, 2008 ).…”

Section: Resultsmentioning

confidence: 95%

Enhanced Production of β-Caryophyllene by Farnesyl Diphosphate Precursor-Treated Callus and Hairy Root Cultures of Artemisia vulgaris L.

et al. 2021

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Artemisia vulgaris L. produces a wide range of valuable secondary metabolites. The aim of the present study is to determine the effects of various concentrations of farnesyl diphosphate (FDP) on β-caryophyllene content in both callus and hairy root (HR) cultures regeneration from leaf explants of A. vulgaris L. Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4D; 4–13 μM), α-naphthaleneacetic acid (NAA; 5–16 μM), and FDP (1 and 3 μM) was used for callus induction and HR regeneration from leaf explants of A. vulgaris L. In this study, precursor-treated (2,4D 13.5 μM + FDP 3 μM) callus displayed the highest biomass fresh weight (FW)/dry weight (DW): 46/25 g, followed by NAA 10.7 μM + FDP 3 μM with FW/DW: 50/28 g. Two different Agrobacterium rhizogenes strains (A4 and R1000) were evaluated for HR induction. The biomass of HRs induced using half-strength MS + B5 vitamins with 3 μM FDP was FW/DW: 40/20 g and FW/DW: 41/19 g, respectively. To determine β-caryophyllene accumulation, we have isolated the essential oil from FDP-treated calli and HRs and quantified β-caryophyllene using gas chromatography–mass spectrometry (GC–MS). The highest production of β-caryophyllene was noticed in HR cultures induced using A4 and R1000 strains on half-strength MS medium containing 3 μM FDP, which produced 2.92 and 2.80 mg/ml β-caryophyllene, respectively. The optimized protocol can be used commercially by scaling up the production of a β-caryophyllene compound in a short span of time.

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Section: Resultsmentioning

confidence: 95%

Enhanced Production of β-Caryophyllene by Farnesyl Diphosphate Precursor-Treated Callus and Hairy Root Cultures of Artemisia vulgaris L.

et al. 2021

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“…Plant tissue culture techniques being independent of climatic and geographical conditions will provide an incessant, sustainable, economical and viable production of secondary metabolites 17 . Cell suspension culture of Spilanthe sacmella Murr used successfully to produce scopoletin 18 . It has been mentioned that Callus induction and multiplication have been extensively used in Product Development and Manufacturing Center "PDMC" in vitro production.…”

Section: Resultsmentioning

confidence: 99%

Saponins, Glycosides and Flavonoids in Cells and Tissues of Balanites Aegyptiaca Cultured on Solid and Liquid Culture Media

et al. 2020

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Objectives: The main objective of the present study is to obtain callus and cell suspension culture from Balanitesa egyptiaca sterile plantlets grown in vitro and to compare growth and the biosynthetic potential of saponins, flavonoids and glycosides by callus and cell suspension culture Balanitisa egyptiaca. Methods: Callus was induced from the mother plants on MS culture media supplemented with 2.0 mg/l BA + 2.0 mg/l 2,4-D with and without agar gelling. Total saponins, glycosides and flavonoids were estimated in both types of cultures over a period extending from 1 to 5 weeks to compare the productivity of such secondary metabolites in callus and cell suspension cultures. Results: The results obtained indicated that both calli and cell suspension cultures were able to synthesize the target active ingredients and that cell suspension culture was superior to the callus culture in the biosynthesis and accumulation processes. By the end of the incubation period, the amount of total saponins in cell suspension culture reached up 51.97±0.26 dry biomass compared to 35.02 ±0.06 mg/g in callus culture. The amount of total flavonoids in cell suspension culture reached up 10.88±0.24 dry biomass compared to 6.40±0.02 mg/g in callus culture and of total glycosides reached up 6.11±0.25 dry biomass compared to 5.06 ±0.05 mg/g in callus culture. Conclusions: The results obtained in this study may indicate the promising role that plant cell culture will play in the future in phytopharmaceutical industry. Peer Review History: Received 16 July 2020; Revised 12 August; Accepted 26 August, Available online 15 September 2020 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.5/10 Reviewer(s) detail: Name: Dr. Gehan Fawzy Abdel Raoof Kandeel Affiliation: Researcher at pharmacognosy Department, National Research Centre, Egypt. E-mail: gehankandeel9@yahoo.com Name: Ahmad Najib Affiliation: Department of Pharmacognosy-Phytochemystry Universitas Muslim Indonesia-Indonesia E-mail: ahmad.najib@umi.ac.id Comments of reviewer(s): Similar Articles: PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY OF COLOCASIA ESCULENTA (TARO) MEDICINAL PLANT LEAVES USED IN FOLK MEDICINE FOR TREATMENT OF WOUNDS AND BURNS IN HUFASH DISTRICT AL MAHWEET GOVERNORATE–YEMEN PHYTOCHEMICAL SCREENING AND THIN LAYER CHROMATOGRAPHIC OF PRUNUS DULCIS (ALMOND) MEDICINAL PLANT LEAVES USED IN FOLK MEDICINE FOR TREATMENT OF WOUNDS AND BURNS IN HUFASH DISTRICT AL MAHWEET GOVERNORATE–YEMEN

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“…Adding organic supplements is another approach to increase the yield of secondary metabolites in plant cell culture systems, as shown in antitoothache plant (Spilanthes acmella) suspension cells (Abyari et al 2016). In the present study, CH was added to stimulate secondary metabolite production in callus and suspension cells.…”

Section: Metabolite Profiles Of Callus and Suspension Cellsmentioning

confidence: 99%

Metabolite profiles of callus and cell suspension cultures of mangosteen

et al. 2018

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Callus was induced from mangosteen ( L.) young purple-red leaves on Murashige and Skoog basal medium with various combinations of plant growth regulators. Murashige and Skoog medium with 4.44 µM 6-benzylaminopurine and 4.52 µM 2,4-dichlorophenoxyacetic acid was the best for friable callus induction. This friable callus was used for the initiation of cell suspension culture. The effects of different combinations of 6-benzylaminopurine and 2,4-dichlorophenoxyacetic acid, carbon sources and inoculum sizes were tested. It was found that combination of 2.22 µM 6-benzylaminopurine + 2.26 µM 2,4-dichlorophenoxyacetic acid, glucose (30 g/l) and 1.5 g/50 ml inoculum size was the best for cell growth. Callus and cell suspension cultures were then treated either with 100 µM methyl jasmonate as an elicitor for 5 days, or 0.5 g/l casein hydrolysate as an organic supplement for 7 days. Metabolites were then extracted and profiled using liquid chromatography-time of flight mass spectrometry. Multivariate discriminant analyses revealed significant metabolite differences ( ≤ 0.05) for callus and suspension cells treated either with methyl jasmonate or casein hydrolysate. Based on MS/MS data, methyl jasmonate stimulated the production of an alkaloid (thalsimine) and fatty acid (phosphatidyl ethanolamine) in suspension cells while in callus, an alkaloid (thiacremonone) and glucosinolate (7-methylthioheptanaldoxime) was produced. Meanwhile casein hydrolysate stimulated the production of alkaloids such as 3ß,6ß-dihydroxynortropane and cis-hinokiresinol and triterpenoids such as schidigerasaponin and talinumoside in suspension cells. This study provides evidence on the potential of secondary metabolite production from in vitro culture of mangosteen.

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Enhanced Accumulation of Scopoletin in Cell Suspension Culture of Spilanthes acmella Murr. Using Precursor Feeding

Abstract: In this study, the various concentrations of casein hydrolysate (25, 50, 75, 100 mg/L) and L-phenylalanine (50, 100, 150, 200 µM/l)

Cited by 22 publications

References 22 publications

Enhanced Production of β-Caryophyllene by Farnesyl Diphosphate Precursor-Treated Callus and Hairy Root Cultures of Artemisia vulgaris L.

Enhanced Production of β-Caryophyllene by Farnesyl Diphosphate Precursor-Treated Callus and Hairy Root Cultures of Artemisia vulgaris L.

Saponins, Glycosides and Flavonoids in Cells and Tissues of Balanites Aegyptiaca Cultured on Solid and Liquid Culture Media

Metabolite profiles of callus and cell suspension cultures of mangosteen

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