Cumin (Cuminum cyminum L.), Fennel (Foeniculum vulgare L.) and Longleaf (Falcaria vulgaris Bernh) that all belong to Apiaceae family as medicinal plants are very important in many countries. Study of genetic diversity for medicinal plant is important for researches in future. One of the methods to evaluate plant genetic diversity and classification of them is the electrophoresis of seed storage proteins. This research was conducted in order to evaluate seed protein variability in different Iranian Cumin, Fennel and Longleaf accessions and grouping them based on these proteins as a biochemical marker. For this purpose, the samples were first powdered in liquid nitrogen and seed protein was extracted with extraction buffer. Then total soluble proteins were resolved on 12.5 % sodium dodecyl sulphate polyacrylamide gel electrophoresis gels. The electrophoretic protein pattern showed 38 bands that were low polymorphism among the accessions. The result of cluster analysis showed that the accessions were classified in three groups (all 29 Cumin accessions in the first group, three Fennel ecotypes in second group and three Longleaf accessions in the last one).
Freezing stress is an important abiotic stress that limiting the yield and
the spatial distribution of many important crops. This study was undertaken
to screen 136 doubled haploid (DH) lines of camelina (Camelina sativa L.)
along with four canola (Brassica napus) cultivars (Hyola 401, Lord, Roska and
Cascade) as experimental control under freezing stress conditions (-14?C for
6 h) to identify lines with high or low level of tolerance to freezing stress
for further studies. First, a protocol was developed for large scale
screening of camelina germplasm under freezing stress conditions. For this
purpose, an experiment with different freezing temperatures (-5, -10, -15 and
-20?C) was conducted to find an appropriate temperature that discriminated
best between genotypes (i.e. the LT50 temperature). The LT50 values for
camelina lines were varied between -10.2 and -17.1?C with an average of
-13.94?C for all of the camelina lines. Therefore, we selected the -14?C
exposure for 6 h as an appropriate temperature to screening of camelina
lines. The principal components of measured parameters (LT50, survival
percentage, relative conductivity and scoring) was using principal component
analysis that determine freezing-tolerant and freezing-sensitive lines. Among
136 doubled haploid lines, some lines (58, 62 and 101) had higher level of
freezing tolerance and some of them (8, 16, 32, 91 and 107) were freezing
sensitive. The selected lines in a preliminary freezing screening are useful
for further evaluations.
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