Assessment of Secondary Metabolites in In vitro Regenerated Plantlets of Oroxylum indicum (L.) Vent

Abstract

Flavonoids baicalein and baicalein-7-o-glucoside were estimated in in vitro raised (six months old) as well as nature grown (12 months old) plants of Oroxylum indicum (L.) Vent. Multiple shoot formation and elongation were obtained from axillary and apical bud explants of O. indicum on MS containing BAP (1 mg/l) and AgNO3 (2 mg/l). Rooting of the regenerated shoots was achieved on medium (half strength) containing IBA (1 mg/l) and AgNO3 (1 mg/l). A quantitative HPLC analysis of methanolic extract of leaf and root indicated the enhancement of baicalein-7-o glucoside in in vitro raised root and leaf of Oroxylum indicum.

Effect of the ethylene inhibitors silver nitrate, silver sulfate, and cobaltchloride on micropropagation and biochemical parameters in the cherryrootstocks CAB-6P and Gisela 6

“…In the CAB-6P rootstock, 40 µM AgNO 3 increased root number to its maximum, whereas 50 µM AgNO 3 gave the longest roots. Our results are in agreement with those reported for Decalepis hamiltonii plants, where the addition of 40 µM AgNO 3 resulted in root initiation and elongation (Bais et al, 2000a), and for Oroxylum indicum (L.) Vent plants, where 1 mg L -1 AgNO 3 combined with 1 mg L -1 IBA resulted in a significant increase of root number and root length (Gokhale and Bansal, 2010). Similarly, in Gentiana lutea plants, AgNO 3 (0.5-3 mg L -1 ) increased root length (Petrova et al, 2011).…”

Stimulation of the production of hypericins in in vitro seedlings of Hypericum adenotrichum by some biotic elicitors

“…H. adenotrichum can be used for studies related to the genus Hypericum in view of the above information.The production, consumption, and international trade in medicinal plants and phytomedicine (herbal medicine) have grown and are expected to grow further in the future. To satisfy growing market demands, surveys are being conducted to unearth new plant sources of herbal remedies and medicines and at the same time develop new strategies for better yield and quality (Gokhale & Bansal, 2010). Plant tissue culture has an important role in the production…”