Preparation of Microcrystalline Cellulose from Water Hyacinth Powder by Enzymatic Hydrolysis Using Cellulase of Local Isolate

Abstract

Introduction: Microcrystalline cellulose is one of the cellulose derivatives widely used in the pharmaceutical industry as an excipient in the manufacture of tablets. Unfortunately, most of these filler materials are still largely imported. On the other hand, Indonesia has huge potential to generate microcrystalline cellulose from agricultural waste or weeds such as oil palm empty fruit bunch and water hyacinth. The purpose of this study was to find a potential cellulolytic mold and raw material for preparation of microcrystalline cellulose by enzymatic hydrolysis. Method: The potential cellulolytic mold was obtained from rotted oil palm trunk, and alpha cellulose was prepared by digesting raw material powder by the alkaline condition. Cellulase enzymes are obtained through extraction from the cellulolytic mold and used to treat alpha cellulose at a concentration of 2, 6 and 10 % v/v, respectively. Then, the resulted microcrystalline cellulose was identified by SEM (Scanning Electron Microscope) and XRD (X-Ray Diffraction) and compared with reference Avicel pH 101. Results: The results showed that crude enzyme of isolated mold has better activity than

CHARACTERIZATION OF HYDROXYPROPYL CELLULOSE PRODUCED FROM Α-Cellulose BETUNG BAMBOO (DENDROCALAMUS ASPER) AND IT’S APPLICATION IN TABLET FORMULATION

“…This process causes the sample to emit secondary electrons that bounce can be detected by a detector scientor and amplified by an electrical circuit that produces an image on cathode ray tube (CRT). Image shooting is done after selecting a specific part of the sample and the desired magnification in order to obtain a good and clear image [17].…”

Studies on Microcrystalline Cellulose Obtained From Saccharum Officinarum 2: Flow and Compaction Properties

“…Microcrystalline cellulose also exhibits a high dilution potential which makes it suitable for the formulation of low dose and potent drugs. Besides wood pulp and cotton linters, literature search shows that MCC has been prepared from other sources such as Indian bamboo 3 , groundnut husk 4 , cotton stalk pulps 5 , hosiery waste 6 , corncobs 7 , Sorghum caudatum 8 , water hyacinth 9 and Saccharum officinarum officinarum commonly called sugar cane is a large, tall plant of the perennial grass species (Family: Poaceae) that grows in clumps 11 . Although many species exist, more popular are the species with a green, purple or pinkish coloured stem.…”

Characterization of Kapok Pericarpium Microcrystalline Cellulose Produced of Enzymatic Hydrolysis Using Purified Cellulase From Termite (Macrotermes Gilvus)

“…KBr 100 mg was weighed for a blank and a baseline. A Scanning was performed in 4000 cm -1 to 400 cm -1 Crystal analysis was performed using X-ray diffraction (XRD) (Rigaku Miniflex 600) [5]. wave number area [12].…”

“…In common, termites can eat all of the materials that obtained cellulose. Preparation of MCC from kapok pericarpium powder by enzymatic reaction has already performed by Suryadi et al [5]. In our previous study, the enzyme that used was still in the form of crude enzymes while in this study using the purified enzyme.…”