Premieres Caracteristiques De La Souche Sersenk Du Virus De La Variole De La Chevre

Tantawi, H. H.; Awad, M. M.; Shony, M. O.; Alwan, Ali Hussein; Hassan, Farrukh

doi:10.1007/bf02242628

Cited by 8 publications

(3 citation statements)

References 4 publications

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“…Chloroform and ether treatments resulted in complete inactivation of GPV-LK. This high sensitivity to lipid solvents, mainly to ether, seems to be a specific characteristic for GPV-LK since several investigators indicated partial inactivation of GPV by ether (4,13,17).…”

Section: Resultsmentioning

confidence: 96%

“…Views on the degree of relatedness between I 544 TALHOUK and EL-ZEIN the two viruses have been conflicting: absence of an antigenic relationship between sheep pox virus (SPV) and goat pox virus (GPV) (3) as well as the presence of partial (14,15,16,18) and complete (2, 4, 6) antigenic cross-reactivity have been reported. Pathogenic species-specific viruses have been described (9,17). A strain of equal pathogenicity for sheep and goats was isolated by DAVIES (4) b u t the growth of both viruses in heterologous hosts is admitted by all investigators.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a Cell Culture Adapted Goat Pox Virus¹

Talhouk¹,

el-Zein²

1986

Journal of Veterinary Medicine, Series B

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Summary An attenuated goat pox virus (GPV) in sheep testicle cell (STC) culture was further adapted to sheep embryo dermis cell (SEDC) culture and was characterized in terms of its growth pattern and its resistance to physical and chemical treatments. A single growth curve in SEDC showed an eclipse period of less than 18 hrs, followed by exponential growth with its maximum at 4 days post‐infection. Thereafter, a plateau was established till 11 days post‐infection with an insignificant fall in virus titre occurring after 9 days post‐infection. In the following two weeks of incubation the total fall in titre slightly exceeded 1 log. Intracellular virus was much higher than extracellular virus during the exponential growth; it did remained higher up to 18 days post‐infection, when intra‐ and extracellular virus were equal in amount. The strain was resistant to heat: 450 and 30 minutes, respectively, were needed to inactivate the strain at 50 °C and 56 °C. Chloroform and ether caused complete inactivation of the strain. Trypsin caused gradual inactivation of the attenuated GPV, but complete inactivation was not achieved before 2 hours of treatment with the proteolytic enzyme in association with ethylenediamine‐tetraacetic acid (EDTA). Zusammenfassung Charakterisierung eines Zellkultur‐adaptierten Ziegenpockenvirus Ein in Schafhoden‐Zellkulturen (STC) attenuiertes Ziegenpockenvirus (GPV) wurde anschließend an embryonale Schafhaut‐Zellkulturen (SEDC) adaptiert und in bezug auf sein Ver‐mehrungsverhalten und seine Widerstandsfähigkeit gegenüber physikalischen und chemischen Behandlungen charakterisiert. Die Einschrittvermehrungskurve in SEDC ließ eine Eklipsephase von weniger als 18 Stunden mit anschließender exponentieller Vermehrung des Virus erkennen, die in einem Maximum am 4. Tag p. inf. gipfelte. Danach stellte sich ein Plateau ein mit einem nicht signifikantem Titerabfall am 11. Tag. In den darauffolgenden 2 Wochen Inkubation erreichte die Reduktion des Virustiters etwas mehr als 1 log 10. Während der exponentiellen Vermehrung war der Gehalt an intrazellulärem Virus viel höher als an extrazellulärem; er blieb bis zum 18. Tag p. inf. höher und war dann dem extrazellulären Gehalt gleich. Der Stamm verhielt sich wärmeresistent: zur Inaktivierung waren 450 bzw. 30 min Inkubation bei 50 bzw. 56 °C notwendig. Chloroform und Äther inaktivierten den Stamm restlos. Trypsin bewirkte eine nur graduelle Inaktivierung des attenuierten GPV. Durch eine Behandlung mit diesem proteolytischen Enzym in Verbindung mit Äthylendiamintetraessigsäure (EDTA) ließ sich eine vollständige Inaktivierung nicht unter 2 Stunden erreichen.

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Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Characterization of a Cell Culture Adapted Goat Pox Virus¹

Talhouk¹,

el-Zein²

1986

Journal of Veterinary Medicine, Series B

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“…This result agrees with Onar (1972), Sen and Uppal (1972), Sharma and Dhanda (1972) and Bhatnagar and Gupta (1974), who unable to propagate it in chick embryos. SPPV isolates differ significantly in their growth behavior in embryonated chick eggs (Adlakha et al, 1971;Tantawi and Al Falluji, 1979;Rao and Bandyopadhyay, 2000).…”

Section: Discussionmentioning

confidence: 99%

An Epidemiological Study of Sheep and Goat pox Outbreaks in the Sudan

Mahmoud¹,

Khalafalla²,

Abdellatif³

2016

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Sheep pox (SP) and goat pox (GP) outbreaks occurred in the different geographic areas of Sudan and most strikingly, were highly species specific. Two outbreaks in Gedaref State in June 2013 affected no goats and outbreak in Khartoum state in March 2015 affected no sheep despite communal herding; affected goats were vaccinated with 0240 strain. Clinically, the disease was characterized by fever, depression, and eruption of generalized pox lesions. Mortality rate ranged between 5.2% and 6.7% with a mean of 6.1%. Isolation of viruses succeed on lamb testes cell culture at passage four; the diseases were diagnosed using virus neutralization test and polymerase chain reaction. SP and GP isolates grew well in lamb testes and vero cells. In Madin-Darby bovine kidney; however, both viruses induced slight cytopathic effect (CPE) that reached 60% in 9 days. On the other hand, both isolates induced no CPE in chick embryo fibroblast cells. Virus isolation attempts failed on chorioallantoic membrane of embryonated chicken eggs.

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