Potensi Fraksi Etil Asetat Ekstrak Daun Gambir (Uncaria gambir Roxb.) sebagai Antihiperlipidemia

Yunarto, Nanang; Elya, Berna; Konadi, Laurentia

doi:10.22435/jki.v5i1.4080.1-10

Cited by 12 publications

(12 citation statements)

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“…The ethyl acetate fraction layer was evaporated using a rotary evaporator until thick. The ethyl acetate fraction obtained was evaporated in a fume hood, then continued drying in a vacuum oven at 40-50 ° C until a fixed weight was obtained [9].…”

Section: Fractionationmentioning

confidence: 99%

Anti-Inflammatory Activities of Ethyl Acetate Fraction From Uncaria Gambir Leaves Through the Inhibition of Edema, COX-2 and iNOS Expression

Yunarto¹,

Intan²,

Kurniatri³

et al. 2020

Proceedings of the 4th International Symposium on Health Research (ISHR 2019)

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Inflammatory is a body defense mechanism due to respond of tissue on undermine influences, either local or the one that go into the body. Inflammatory can cause various physical dysfunctions. Uncaria gambir Roxb, a high catechin content in ethyl acetate fraction, is widely used as wound healing in Indonesian culture, therefore its mechanism of anti-inflammatory action is still unknown. The objective of our study is to investigated the activity of anti-inflammatory ethyl acetate fraction of Uncaria gambir leaves inhibition of edema, COX-2 and iNOS expression using induction method with carrageenan in rats. This study is experimental research which use 25 white Wistar strain rats as animals assay that divided into 5 groups: negative group (water), positive group (sodium diclofenac 50 mg/kg bw), dose I (5 mg/kg bw fraction), II (10 mg/kg bw fraction) and III (20 mg/kg bw fraction) groups. Each of them were given orally 30 min before carrageenan 3% induced. The paw volume was measured every day for seven day after injection carrageenan using plethysmometer. Inhibition COX-2 and iNOS expression were determined by enzyme-linked immunosorbent assay. The results showed that ethyl acetate fraction of Uncaria gambir leaves in all doses had anti-inflammatory effect on white rats through the mechanism of inflammatory inhibition by reducing edema volume. All three fraction doses can also reduce COX-2 and iNOS expression. The fraction had the highest anti-inflammatory potency by the dose of 20 mg/kg body weight. The conclusion that ethyl acetate of three doses had anti-inflammatory activities through a mechanism of edema volume reduction, COX-2 and iNOS expression.

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Section: Fractionationmentioning

confidence: 99%

Anti-Inflammatory Activities of Ethyl Acetate Fraction From Uncaria Gambir Leaves Through the Inhibition of Edema, COX-2 and iNOS Expression

Yunarto¹,

Intan²,

Kurniatri³

et al. 2020

Proceedings of the 4th International Symposium on Health Research (ISHR 2019)

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“…8,9,10 Katekin dari gambir juga dapat dikembangkan potensinya sebagai antidislipidemia. 11,12 Penelitian secara praklinik menunjukkan adanya korelasi positif antara konsumsi katekin dalam teh hijau dengan kesehatan jantung melalui banyak mekanisme yaitu antioksidasi, antihipertensi, antiinflamasi, antiproliferasi, antitrombogenik, dan menurunkan kadar lemak. 13 Mekanisme katekin dalam menghambat aktivitas enzim CoA reduktase yang berperan dalam sintesis kolesterol telah ditelusuri secara in silico dan ditemukan bahwa katekin lebih efektif dibandingkan dengan simvastatin.…”

Section: Pendahuluanunclassified

Purifikasi Katekin dari Ekstrak Gambir (Uncaria gambir Roxb.)

Kurniatri

Sulistyaningrum

Rustanti

2019

mpk

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Abstract Uncaria gambir Roxb., one of native plants of Indonesia contains high levels of catechins. Catechins are very potential to be used for medicinal raw materials because theirs effects are proven to be antibacterial, antiviral, and antidyslipidemic. Derivatization of catechin can be developed to produce drug compounds that are effective as antiviral agents for HIV. Derivatization process needs pure catechin isolate as a starting material in order to obtain maximum result so that the isolation process is one of the important stages. In this study, isolation and purification of catechin isolate from Uncaria gambir Roxb. extract was carried out, which will then be the starting material in the derivatization of catechin. Gambir extract used for catechin isolation was a quality 1 gambir extract obtained from Padang, West Sumatra. Gambier extract is characterized according to the standard method stated in Farmakope Herbal Indonesia. Isolation of catechin from gambir extract was done by percolation method using ethyl acetate solvents. Pure catechin isolate was obtained using a vacuum liquid chromatograpy method with a series of solvent hexane and ethyl acetate gradients. Catechin purification was monitored using Thin Layer Chromatography (TLC) method with eluent chloroform:ethyl acetate:formic acid (5:4:1, then identified using High Performance Liquid Chromatography (HPLC), Nuclear Magnetic Resonance (NMR) Spectroscopy, and Liquid Cromatography- Mass Spectroscopy (LC-MS). The purity of catechin isolate obtained was 99.80%± 0.132. Abstrak Uncaria gambir Roxb., salah satu tanaman asli Indonesia yang mengandung katekin dengan kadar yang tinggi. Katekin sangat potensial digunakan untuk bahan baku obat karena efeknya terbukti sebagai antibakteri, antivirus, dan antidislipidemia. Derivatisasi katekin dapat dikembangkan untuk menghasilkan senyawa obat yang efektif sebagai antivirus untuk HIV. Untuk derivatisasi ini diperlukan isolat katekin murni sebagai starting material agar diperoleh hasil yang maksimal sehingga proses purifikasi isolat merupakan salah satu tahap yang penting. Dalam penelitian ini dilakukan isolasi dan pemurnian isolat katekin dari ekstrak Uncaria gambir Roxb. yang selanjutnya akan menjadi bahan awal dalam derivatisasi katekin. Ekstrak gambir yang digunakan untuk isolasi katekin adalah ekstrak gambir kualitas 1 yang diperoleh dari Padang, Sumatera Barat. Ekstrak gambir dikarakterisasi sesuai dengan metode standar yang tertera dalam Farmakope Herbal Indonesia. Isolasi katekin dari ekstrak gambir dilakukan dengan metode perkolasi menggunakan pelarut etil asetat. Isolat katekin murni diperoleh menggunakan metode kromatografi cair vakum (KCV) dengan serangkaian gradien pelarut heksana dan etil asetat. Pemurnian katekin dimonitor menggunakan metode kromatografi lapis tipis (KLT) dengan eluen kloroform : etil asetat : asam format (5:4:1), kemudian diidentifikasi menggunakan High Performance Liquid Chromatography (HPLC), spektroskopi Nuclear Magnetic Resonance (NMR), dan Liquid Cromatography-Mass Spectroscopy (LC- MS). Kemurnian isolat katekin yang didapatkan 99,80%± 0,132.

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“…Penelitian Yunarto et al menyebutkan bahwa fraksi etil asetat ekstrak daun gambir dapat mengurangi kadar kolesterol total, trigliserida, LDL, dan meningkatkan HDL dalam plasma darah tikus pada dosis 20mg/200g BB. 9 Oleh karena itu, dilakukan penelitian untuk mengetahui mekanisme kerja katekin dalam mengatasi dislipidemia dengan menggunakan metode komputasi.…”

Section: Mekanisme Katekin Sebagai Obat Antidislipidemiaunclassified

Mekanisme Katekin Sebagai Obat Antidislipidemia (Uji In Silico)

Adelina

2018

bpk

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Indonesia is rich in medicinal plants, such as Uncaria gambir Linn which can be used as a source of medicinal raw materials. The high content of catechin in "gambir" has the potential as an antidyslipidemic drug. Pre clinic trial results showed that Gambir's leaves containing ethyl acetate fraction decreased total cholesterol, triglyceride and LDL level and also improve HDL level. The mechanism of catechin as antidyslipidemic drug can be traced using a molecular docking study which is one of the studies of the in silico study model used to filter compounds based on their mechanism of action against targeted proteins. In this study, the molecular docking of catechin was done using Molecular on Environment Software (MOE) to identify the affinity and interaction with HMG-CoA reductase and LDL enzymes that contribute to fat/cholesterol metabolism. The results of molecular docking showed that catechin interaction against HMG-CoA reductase and LDL receptor enzymes had Gibbs value of -6,5758 kcal/mol and -16,1709 kcal/mol, respectively. Potential catechin action mechanisms as antidyslipidemic used two pathways, inhibition of the HMG-CoA reductase enzyme and increase the LDL receptor. AbstrakIndonesia kaya akan tanaman obat, salah satunya Uncaria gambir Linn yang dapat digunakan sebagai sumber bahan baku obat. Kandungan senyawa katekin yang tinggi dalam gambir berpotensi sebagai antidislipidemia. Hasil uji pre-klinik menunjukkan bahwa fraksi etil asetat ekstrak daun gambir dapat mengurangi kadar kolesterol total, trigliserida, LDL, dan meningkatkan HDL. Mekanisme katekin sebagai antidislipidemia dapat ditelusuri menggunakan studi docking molekuler yang merupakan salah satu studi model studi in silico yang digunakan untuk menapis senyawa berdasarkan mekanisme kerjanya terhadap protein target. Pada penelitian ini senyawa katekin dilakukan docking secara molekuler dengan menggunakan Software Moleculer on Environtment (MOE) dengan tujuan untuk mengetahui daya afinitas dan interaksinya terhadap enzim HMG-KoA reduktase dan reseptor LDL yang berperan terhadap metabolisme kolesterol. Hasil docking molekuler menunjukkan bahwa interaksi katekin terhadap enzim HMG-KoA reduktase dan reseptor LDL memiliki nilai Gibbs masing-masing sebesar -6,5758 kacl/mol dan -16,1709 kcal/mol. Potensi mekanisme aksi katekin sebagai antidislipidemia menggunakan dua jalur yaitu penghambatan enzim HMG-KoA reduktase dan peningkatan reseptor LDL.

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Potensi Fraksi Etil Asetat Ekstrak Daun Gambir (Uncaria gambir Roxb.) sebagai Antihiperlipidemia

Cited by 12 publications

References 0 publications

Anti-Inflammatory Activities of Ethyl Acetate Fraction From Uncaria Gambir Leaves Through the Inhibition of Edema, COX-2 and iNOS Expression

Anti-Inflammatory Activities of Ethyl Acetate Fraction From Uncaria Gambir Leaves Through the Inhibition of Edema, COX-2 and iNOS Expression

Purifikasi Katekin dari Ekstrak Gambir (Uncaria gambir Roxb.)

Mekanisme Katekin Sebagai Obat Antidislipidemia (Uji In Silico)

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