Purifikasi Katekin dari Ekstrak Gambir (Uncaria gambir Roxb.)

Kurniatri, Arifayu Addiena; Sulistyaningrum, Novi; Rustanti, Lina

doi:10.22435/mpk.v29i2.1108

Cited by 13 publications

(9 citation statements)

References 11 publications

(11 reference statements)

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“…During the process, ethyl acetate is added through the top of the column. The fraction results were concentrated with a rotary evaporator at a temperature of 40°C, then dried using a vacuum oven at 40°C to a fixed weight (Yunarto et al, 2017;Kurniatri et al, 2019). The obtained fraction was calculated to yield fraction, organoleptic testing, moisture content, and ash content.…”

Section: Fractionationmentioning

confidence: 99%

VALIDATION OF SPECTROPHOTOMETRY METHOD FOR DETERMINATION OF (+)-CATECHIN IN ETHYL ACETATE FRACTION OF GAMBIR EXTRACT (Uncaria gambir Roxb.)

Yunarto

Reswandaru

Sulistyowati³

et al. 2021

J. Tumbuhan Obat Indonesia

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ABSTRACT Gambir leaves (Uncaria gambir Roxb.) is a plant that has been widely used by people for traditional medicine. The main compound of gambir extract is a (+)-catechin that has been proven as anti-dental plaque, antioxidant, antibacterial, and antihyperlipidemic. This study aims to validate a method for the quantitative determination of catechin in ethyl acetate fraction of gambir extract based on spectrophotometry. The validation was conducted by measuring the linearity, accuracy, and ruggedness of the method that fulfills the requirements along with the limit of detection (LoD) and limit of quantification (LoQ) determination. Determining the precision (based on %RSD and CV), 100 ppm of catechin were made to 7 replicates while accuracy was evaluated by calculating the recovery. The ruggedness of the method is determined by analyzing it on a different day. The linearity was determined by assessing the r-value on the relation between the absorbance curve and standard series concentration. The LoD and LoQ could be calculated based on the standard curve equation. The results showed the method fulfilled the linearity requirement with R 0.9996; had precision in 1.23% RSD; had accuracy in the range of 100.80% to 101.64%; the method ruggedness was not significantly different; had 3.85 ppm of LoD and 12.84 ppm of LoQ. The validation method showed a valid result, and the method can be used for routine analysis on the measurement of catechin in ethyl acetate fraction of gambir extract. Keywords: Catechin, Uncaria gambir, Method Validation, Spectrophotometry ABSTRAK Daun gambir (Uncaria gambir Roxb.) merupakan tanaman yang telah banyak dimanfaatkan masyarakat dalam pengobatan tradisional. Senyawa utama ekstrak gambir adalah (+)-katekin yang telah terbukti sebagai anti plak gigi, antioksidan, antibakteri, dan antihiperlipidemia. Penelitian ini bertujuan untuk memvalidasi suatu metode penentuan kuantitatif katekin dalam fraksi etil asetat ekstrak gambir secara spektrofotometri. Validasi dilakukan dengan mengukur linearitas, akurasi, dan kekasaran metode yang memenuhi persyaratan beserta penetapan Limit of Detection (LoD) dan Limit of Quantification (LoQ). Penentuan presisi (berdasarkan %RSD dan CV), 100 ppm katekin dibuat 7 ulangan sedangkan akurasi dievaluasi dengan menghitung perolehan kembali. Kekasaran metode ditentukan dengan menganalisisnya pada hari yang berbeda. Linearitas ditentukan dengan mengukur nilai r pada hubungan antara kurva absorbansi dan konsentrasi seri standar. Berdasarkan persamaan kurva standar, maka LoD dan LoQ dapat dihitung. Hasil penelitian menunjukkan metode memenuhi syarat linearitas dengan R 0,9996; memiliki presisi di 1,23% RSD; memiliki akurasi pada kisaran 100,80% sampai dengan 101,64%; kekasaran metode tidak berbeda nyata; memiliki 3,85 ppm LoD; dan 12,84 ppm LoQ. Validasi menunjukkan hasil yang valid, dan metode tersebut dapat digunakan untuk analisis rutin pada pengukuran katekin dalam fraksi etil asetat ekstrak gambir. Kata kunci: Catechin, Uncaria gambir, Validasi Metode, Spektrofotometri

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Section: Fractionationmentioning

confidence: 99%

VALIDATION OF SPECTROPHOTOMETRY METHOD FOR DETERMINATION OF (+)-CATECHIN IN ETHYL ACETATE FRACTION OF GAMBIR EXTRACT (Uncaria gambir Roxb.)

Yunarto

Reswandaru

Sulistyowati³

et al. 2021

J. Tumbuhan Obat Indonesia

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“…Bajakah kalalawit is one of Indonesia's export commodities which has high economic value. Indonesia is the largest supplier of kalalawit steel in the world, namely 80%, and 90% of Indonesia's supply of kalalawit steel is produced from West Sumatra 3 .…”

confidence: 99%

Analysis of Total Flavonoid Content in the Extract of Bajakah Kalalawit Root (Uncaria gambir Roxb) Infunded Results

Alfani¹,

Febrianti²,

Amananti³

2023

IJCST

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Bajakah kalalawit is one of the plants that has been empirically used in traditional medicine. The roots of Bajakah kalalawit (Uncaria gambir Roxb) have phenol and antibacterial content and have a high enough content of flavonoid compounds so that they have pharmacological activity as an anticancer. This article examines the content of flavonoid compounds contained in the extract of Bajakah Kalalawi root and also examines the total levels of flavonoids contained in the roots of Bajakah Kalalawi. Bajakah kalalawit root extract was obtained by the infundation extraction method using a water solvent (1:10). Examination of the characteristics of the sample is carried out by macroscopic and microscopic identification. The qualitative test of flavonoids was carried out by color reaction tests in two treatments. Determination of flavonoid levels in the root extract of bajakah kalalawi was carried out by the UV-Vis spectrophotometry method. From the results of the study showed that the levels of flavonoids in the root extract of Bajakah Kalalawi in sample A were 33.2% and in sample B by 44.8%. The results showed that the roots of Bajakah Kalalawi were shown to contain flavonoid compounds.

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“…One of the native plants in Indonesia which contain a large number of catechins is Gambir (Uncaria gambirRoxb.) (Kurniatri et al, 2019). Gambir leaf extract contains catechins as a major component as well as some other components such as catechu tannin acid, quercetin, red catechu, gambir fluorescent, fats, and waxes (Munggari et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of Purified Gambier (Uncaria gambir Roxb.)

Ifora¹,

Bellatasie²,

Efelzita³

2022

Int.J.Res.Publ.Rev.

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The bacterial resistance shown by the microorganisms in the presence of antimicrobial agents has prompted the search for new materials to overcome this public health problem. Gambir (Uncaria gambir, Roxb.) leaves extract has high catechin content thereby potentially antibacterial. This research aimed to examine the effect of purified gambir extract on inhibiting growth and killing bacteria. The antibacterial activity of Purified gambir extract (Uncaria gambir Roxb.) with concentrations of 32%, 16%, and 8% was tested against S. aureus and P. aeruginosa using the agar diffusion method. Purified gambir extract at concentrations of 8%, 16%, and 32% showed antibacterial activity against Staphylococcus aureus with an average inhibition zone diameter of 19.25 mm, 23.45 mm, and 26.25 mm, respectively. Purified gambir extract at concentrations of 8%, 16%, and 32% also showed antibacterial activity against Pseudomonas aeruginosa with an average inhibition zone diameter of 11.55 mm, 15,4 mm, and 18,5 mm, respectively. In conclusion, purified gambir extract (Uncaria gambir Roxb.) has very strong antibacterial activity against Staphylococcus aureus bacteria and strong antibacterial activity against Pseudomonas aeruginosa.

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Purifikasi Katekin dari Ekstrak Gambir (Uncaria gambir Roxb.)

Cited by 13 publications

References 11 publications

VALIDATION OF SPECTROPHOTOMETRY METHOD FOR DETERMINATION OF (+)-CATECHIN IN ETHYL ACETATE FRACTION OF GAMBIR EXTRACT (Uncaria gambir Roxb.)

VALIDATION OF SPECTROPHOTOMETRY METHOD FOR DETERMINATION OF (+)-CATECHIN IN ETHYL ACETATE FRACTION OF GAMBIR EXTRACT (Uncaria gambir Roxb.)

Analysis of Total Flavonoid Content in the Extract of Bajakah Kalalawit Root (Uncaria gambir Roxb) Infunded Results

Antibacterial activity of Purified Gambier (Uncaria gambir Roxb.)

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