VALIDATION OF SPECTROPHOTOMETRY METHOD FOR DETERMINATION OF (+)-CATECHIN IN ETHYL ACETATE FRACTION OF GAMBIR EXTRACT (Uncaria gambir Roxb.)

Abstract


 ABSTRACT
 Gambir leaves (Uncaria gambir Roxb.) is a plant that has been widely used by people for traditional medicine. The main compound of gambir extract is a (+)-catechin that has been proven as anti-dental plaque, antioxidant, antibacterial, and antihyperlipidemic. This study aims to validate a method for the quantitative determination of catechin in ethyl acetate fraction of gambir extract based on spectrophotometry. The validation was conducted by measuring the linearity, accuracy, and ruggedness of the method that fulfills the requirements along with the limit of detection (LoD) and limit of quantification (LoQ) determination. Determining the precision (based on %RSD and CV), 100 ppm of catechin were made to 7 replicates while accuracy was evaluated by calculating the recovery. The ruggedness of the method is determined by analyzing it on a different day. The linearity was determined by assessing the r-value on the relation between the absorbance curve and standard series concentration. The LoD and LoQ could be calculated based on the standard curve equation. The results showed the method fulfilled the linearity requirement with R 0.9996; had precision in 1.23% RSD; had accuracy in the range of 100.80% to 101.64%; the method ruggedness was not significantly different; had 3.85 ppm of LoD and 12.84 ppm of LoQ. The validation method showed a valid result, and the method can be used for routine analysis on the measurement of catechin in ethyl acetate fraction of gambir extract.
 Keywords: Catechin, Uncaria gambir, Method Validation, Spectrophotometry
 
 ABSTRAK
 Daun gambir (Uncaria gambir Roxb.) merupakan tanaman yang telah banyak dimanfaatkan masyarakat dalam pengobatan tradisional. Senyawa utama ekstrak gambir adalah (+)-katekin yang telah terbukti sebagai anti plak gigi, antioksidan, antibakteri, dan antihiperlipidemia. Penelitian ini bertujuan untuk memvalidasi suatu metode penentuan kuantitatif katekin dalam fraksi etil asetat ekstrak gambir secara spektrofotometri. Validasi dilakukan dengan mengukur linearitas, akurasi, dan kekasaran metode yang memenuhi persyaratan beserta penetapan Limit of Detection (LoD) dan Limit of Quantification (LoQ). Penentuan presisi (berdasarkan %RSD dan CV), 100 ppm katekin dibuat 7 ulangan sedangkan akurasi dievaluasi dengan menghitung perolehan kembali. Kekasaran metode ditentukan dengan menganalisisnya pada hari yang berbeda. Linearitas ditentukan dengan mengukur nilai r pada hubungan antara kurva absorbansi dan konsentrasi seri standar. Berdasarkan persamaan kurva standar, maka LoD dan LoQ dapat dihitung. Hasil penelitian menunjukkan metode memenuhi syarat linearitas dengan R 0,9996; memiliki presisi di 1,23% RSD; memiliki akurasi pada kisaran 100,80% sampai dengan 101,64%; kekasaran metode tidak berbeda nyata; memiliki 3,85 ppm LoD; dan 12,84 ppm LoQ. Validasi menunjukkan hasil yang valid, dan metode tersebut dapat digunakan untuk analisis rutin pada pengukuran katekin dalam fraksi etil asetat ekstrak gambir.
 Kata kunci: Catechin, Uncaria gambir, Validasi Metode, Spektrofotometri

Development and Validation of a High-Performance Liquid Chromatography-Based Method for Catechin Isolated from the Leaves of Gambir (Uncaria gambir Roxb)

“…The RSD value from the previous validation was 1.23%. 17 The HPLC method for the measurement of gambir leaf catechin isolates has a high level of precision and an acceptable level of repeatability, according to the %RSD value that satisfies the accuracy test results in this validation.…”

“…13 The previous validation study of the catechin analytical method conducted by Yunarto et al (2021) resulted in a slightly higher % recovery value when compared to the results obtained in the present study, which is in the range of 100.80-101.64%. 17 This study used HPLC, which has better sensitivity than the spectrophotometric approach, although prior studies validated the catechin analytical method using a different technique, namely spectrophotometry. This could explain why the results are different.…”

“…17 A robustness test was not conducted in a previous validation method using the spectrophotometer. 17 Hence, a robustness test was established to validate the analytical method for gambir leaf catechin isolates in this study. Robustness is the capacity of a method to remain unaffected by a variation of its input parameters.…”

Validasi Metode Analisis Penetapan Kadar Protein Ekstrak Ikan Gabus dengan Metode Lowry dan Bromocresol Green

“…Hasil dari penetapan parameter akurasi dinyatakan dengan nilai perolehan kembali (% recovery) dengan syarat keberterimaannya adalah 98-102 %. 17,18 Hasil yang diperoleh pada penetapan parameter ini memenuhi syarat ditandai dengan nilai perolehan kembali yang didapatkan masuk dalam rentang 98-102%. Hasil penetapan parameter akurasi dapat dilihat pada Tabel 3.…”

“…Linearitas dikatakan memenuhi syarat apabila r >0.990. 14,[17][18][19] Hasil uji linearitas dapat dilihat pada Tabel 6, Gambar 3, dan Gambar 4 dibawah.…”