Polysaccharide from <i>Caulerpa lentillifera</i>: extraction optimization with response surface methodology, structure and antioxidant activities

Tian, Hua; Liu, Haifang; Song, Weikang; Zhu, Liang; Yin, Xijie

doi:10.1080/14786419.2019.1700507

Cited by 25 publications

(19 citation statements)

References 39 publications

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“…Hot water extract from C. racemosa showed antiviral and antioxidant activities. Several polysaccharides extracted from Caulerpa have shown antioxidant activities: the C. lentillifera polysaccharide produced by ultrasonic-assisted extraction presented good radical scavenging activities against the DPPH radical [97]. The C. lentillifera polysaccharide antioxidant activity might be influenced by sulfate and uronic acid contents [97].…”

Section: Discussionmentioning

confidence: 99%

“…Several polysaccharides extracted from Caulerpa have shown antioxidant activities: the C. lentillifera polysaccharide produced by ultrasonic-assisted extraction presented good radical scavenging activities against the DPPH radical [97]. The C. lentillifera polysaccharide antioxidant activity might be influenced by sulfate and uronic acid contents [97]. Fernando et al [33] reported DPPH activity, with an IC 50 of >2 mg/mL, of a polysaccharide extracted from C. racemosa.…”

Section: Discussionmentioning

confidence: 99%

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An Analysis of the Nutritional and Health Values of Caulerpa racemosa (Forsskål) and Ulva fasciata (Delile)—Two Chlorophyta Collected from the Philippines

et al. 2020

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Polysaccharides, lipids and amino acid profiles were investigated to understand the nutritional value of Caulerpa racemosa and Ulva fasciata from the Philippines. The results revealed that both species contain high amounts of proteins (8.8–19.9% for C. racemosa and 8.0–11.1% for U. fasciata). The portions of the total amino acids that were essential amino acids (EAAs) (45.28 ± 0.12% for C. racemosa and 42.17 ± 0.12% for U. fasciata) out were comparable to FAO/WHO requirements. Leucine, valine, isoleucine, and lysine are the dominant EAAs in C. racemosa, while leucine, valine, lysine, and phenylalanine are those in U. fasciata. The fatty acid profiles are dominated by monounsaturated fatty acids and polyunsaturated fatty acids in C. racemosa (56.2%), while saturated fatty acids (72.1%) are dominant in U. fasciata. High C18/C20 polyunsaturated fatty acid ratios were recorded in both species. Mineral contents for both seaweeds were within levels considered safe for functional foods. Total pigment content of C. racemosa (140.84 mg/g dw) was almost 20 times higher than that of U. fasciata (7.54 mg/g dw). Hot water extract (HWE) from C. racemosa showed in vitro antiherpetic activity without cytotoxicity. Nutritional characteristics confirmed that C. racemosa could be potentially used as a nutritious and functional food items for human consumption.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

An Analysis of the Nutritional and Health Values of Caulerpa racemosa (Forsskål) and Ulva fasciata (Delile)—Two Chlorophyta Collected from the Philippines

et al. 2020

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“…C. lentillifera is a low-energy food with high contents of dietary fibers, and its polysaccharide content is as high as ≥40% (dry weight) ( Zhang et al, 2020 ). Sulfated polysaccharides isolated from C. lentillifera (CLSPs) have been reported to have antioxidant ( Tian et al, 2019 ), anti-diabetic ( Khairuddin et al, 2020 ), and anticancer activities ( Maeda et al, 2012 ). Moreover, a homogeneous fraction, CLGP4, with the monosaccharide composition of Xyl, Man, and Gal in a percentage ratio of 1.00:2.15:2.40 and a molecular weight of 3877.8 kDa exhibited immunostimulatory effect ( Sun et al, 2018 ; Sun et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Structural characterization and SARS-CoV-2 inhibitory activity of a sulfated polysaccharide from Caulerpa lentillifera

You

Song

Wang

et al. 2022

Carbohydrate Polymers

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Caulerpa lentillifera (Bryopsidophyceae, Chlorophyta) is an edible seaweed attracting great attention for its expansion of farming scale and increasing consumption in these years. In the present study, a sulfated polysaccharide (CLSP-2) was isolated and separated from C. lentillifera , and its chemical structure was elucidated by a series of chemical and spectroscopic methods. Among these methods, mild acid hydrolysis and photocatalytic degradation were applied to release mono- and oligo-saccharide fragments which were further identified by HPLC-MS n analysis, affording the information of the sugar sequences and the sulfate substitution in CLSP-2. Results indicated that the backbone of CLSP-2 was constructed of →6)-β-Man p -(1→ with sulfated branches at C2, which were comprised of prevalent →3)-β-Gal p 4S-(1→, →3)-β-Gal p 2,4S-(1→, and minor Xyl. In addition, the virus neutralization assay revealed that CLSP-2 could effectively protect HeLa cells against SARS-CoV-2 infection with an IC 50 of 48.48 μg/mL. Hence, the present study suggests CLSP-2 as a promising agent against SARS-CoV-2.

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“…Another study reported that the constituent monomers of C. lentillifera from the South China Sea consisted of xylose, galactose, glucose and glucuronic acid (Tian et al 2019). Mannose, galactose, glucose and xylose are the constituent monomers of sulfated polysaccharides in the extracts of C. lentillifera from Phetchaburi Province, Thailand (Chaiklahan et al 2020).…”

Section: Monosaccharide Compositionmentioning

confidence: 99%

Characterization and analysis of the antidiabetic activities of sulphated polysaccharide extract from Caulerpa lentillifera

Fajriah¹,

Rizki²,

Sinurat³

2021

PHAR

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Caulerpa lentillifera is a type of green seaweed that is cultivated in tropical and subtropical areas. The objectives of this study were to determine the characteristics of the sulfated polysaccharides from C. lentillifera and evaluate its antidiabetic activity. In the initial process of this study, samples were macerated with ethanol (1:10). Then, the maceration residue was extracted with an accumulator at 75 °C for three hours. The crude extract yield was 4.16% based on weight seaweed. Ion chromatography purification with DEAE-Sepharose resin provided a yield of 14.8% of crude extract. The monomer analysis of C. lentillifera from the crude extract and purified extract revealed that galactose monomers were dominant and glucose was a minor component. The total carbohydrate and sulfate contents of purified C. lentillifera were higher than those of crude C. lentillifera. Bioactivity tests revealed that purified polysaccharides had higher antidiabetic activity against α-glucosidase enzyme than crude ones with IC50 values of 134.81± 2.0 µg/mL. Purified sulfated polysaccharides of C. lentillifera could potentially be used as an antidiabetic medication.

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Polysaccharide from Caulerpa lentillifera: extraction optimization with response surface methodology, structure and antioxidant activities

Cited by 25 publications

References 39 publications

An Analysis of the Nutritional and Health Values of Caulerpa racemosa (Forsskål) and Ulva fasciata (Delile)—Two Chlorophyta Collected from the Philippines

An Analysis of the Nutritional and Health Values of Caulerpa racemosa (Forsskål) and Ulva fasciata (Delile)—Two Chlorophyta Collected from the Philippines

Structural characterization and SARS-CoV-2 inhibitory activity of a sulfated polysaccharide from Caulerpa lentillifera

Characterization and analysis of the antidiabetic activities of sulphated polysaccharide extract from Caulerpa lentillifera

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