Structural characterization and SARS-CoV-2 inhibitory activity of a sulfated polysaccharide from Caulerpa lentillifera

Abstract

Caulerpa lentillifera (Bryopsidophyceae, Chlorophyta) is an edible seaweed attracting great attention for its expansion of farming scale and increasing consumption in these years. In the present study, a sulfated polysaccharide (CLSP-2) was isolated and separated from C. lentillifera , and its chemical structure was elucidated by a series of chemical and spectroscopic methods. Among these methods, mild acid hydrolysis and photocatalytic degradation were applied to release mono- and oligo-saccharide fragments which were further identified by HPLC-MS n analysis, affording the information of the sugar sequences and the sulfate substitution in CLSP-2. Results indicated that the backbone of CLSP-2 was constructed of →6)-β-Man p -(1→ with sulfated branches at C2, which were comprised of prevalent →3)-β-Gal p 4S-(1→, →3)-β-Gal p 2,4S-(1→, and minor Xyl. In addition, the virus neutralization assay revealed that CLSP-2 could effectively protect HeLa cells against SARS-CoV-2 infection with an IC 50 of 48.48 μg/mL. Hence, the present study suggests CLSP-2 as a promising agent against SARS-CoV-2.

A Novel Polysaccharide from AuriculariaAuricula Alleviates Thrombosis Induced by Carrageenan in Mice

“…The corresponding C-2, C-3, C-4, C-5 and C-6 were assigned to δ 71.07, 72.97, 66.82, 69.16 and 60.66 ppm. These results were consistent with the previous report by You et al [ 24 ].…”

Dietary fibers obtained from Caulerpa lentillifera prevent high-fat diet-induced obesity in mice by regulating the gut microbiota and metabolite profiles

“…C. lentillifera (100 g) was washed with tap water to remove sediments, and neutral protease (0.3%, w/w) was added to hydrolyze proteins at 50 °C for 2 h. Then, the water-soluble dietary fiber (CLP) was extracted by our previously reported method. 3 After that, the precipitate was resuspended in distilled water (100 mL) and centrifuged at 3000 g for 10 min to remove water-soluble materials, and this step was repeated three times. Then, the precipitation was resuspended in ethanol (100 mL) and centrifuged at 3000 g for 10 min to remove alcohol-soluble impurities with three repetitions.…”

“…The analyses for monosaccharide composition, Fourier transform-infrared (FT-IR) spectroscopy, and X-ray diffraction (XRD) of the CL-IDF were carried out according to our previous method. 3 Briefly, for monosaccharide composition, the CL-IDF was hydrolyzed by 2 M trifluoroacetic acid and labeled with 1-phenyl-3-methyl-5-pyrazolone (PMP). After that, the PMP derivative was analyzed by high-performance liquid chromatography (HPLC) equipped with a C18 column (250 mm × 4.6 mm, 5 μm, Dikma, CN) and a photodiode array detector (Thermo Fisher Scientific, Switzerland).…”

“…1 Emerging studies have reported that there are various functional components in C. lentillifera, such as unsaturated fatty acids, caulerpenynes, and dietary fibers. It has been revealed that these functional components exhibit antidiabetic, 2 antiviral, 3 and immunomodulatory capabilities. 4 However, little information is available on the anti-obesity effects.…”

Advances in Research on Antiviral Activities of Sulfated Polysaccharides from Seaweeds

“…In addition, seaweed polysaccharides can inhibit viral transcription and replication through direct interference with viral replication enzymes or inhibition on other intracellular targets [ 140 ]. Marine polysaccharides, especially sulfated polysaccharides from marine algae, appear to block viral infection by inhibition of one or more of these stages, with the specific mechanisms dependent on the structure of the individual polysaccharide [ 140 , 141 , 142 , 143 , 144 ].…”